Chondrogenic Differentiation of Human Mesenchymal Stem Cells on a Patterned Polymer Surface
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Mesenchymal stem cells (MSCs) are an attractive tool in tissue engineering as they have the required potential to treat injured articular cartilage. UV-exposed DTOPV (S-triazine bridged p-phenylene vinylene) is a biocompatible and fluorescent polymer with a hydrophilic surface. Previous studies have demonstrated that the surface wettability and hydrophilicity play critical roles in regulating cell adhesion and proliferation. The objective of this study was to improve the potential of in vitro MSC differentiation into Chondrocytes using DTOPV. MSCs were cultured on two different substrates: (1) tissue culture polystyrene (TCPS) as a reference and (2) UV-exposed and patterned DTOPV films. Chondrogenesis of MSCs was induced for two weeks on TCPS and DTOPV in the presence of an induction medium containing transforming growth factor (TGF)-3. Interestingly, the MSCs on TCPS adhered and spread, while those on DTOPV tended to form aggregates within several days. The cells cultured on DTOPV for two weeks had a round morphology, with stronger Safranine O staining of the extracellular matrix than that of the cells cultured on TCPS. Also, Type II collagen gene was significantly expressed in cells induced on DTOPV. These results indicate that chondrogenic differentiation of MSCs proceeds more rapidly on DTOPV than on TCPS. Therefore, in cartilage tissue engineering, DTOPV could be used to induce effective chondrogenic differentiation of MSCs.
Abstract
Mesenchymal stem cells (MSCs) are an attractive tool in tissue engineering as they have the required potential to treat injured articular cartilage. UV-exposed DTOPV (S-triazine bridged p-phenylene vinylene) is a biocompatible and fluorescent polymer with a hydrophilic surface. Previous studies have demonstrated that the surface wettability and hydrophilicity play critical roles in regulating cell adhesion and proliferation. The objective of this study was to improve the potential of in vitro MSC differentiation into Chondrocytes using DTOPV. MSCs were cultured on two different substrates: (1) tissue culture polystyrene (TCPS) as a reference and (2) UV-exposed and patterned DTOPV films. Chondrogenesis of MSCs was induced for two weeks on TCPS and DTOPV in the presence of an induction medium containing transforming growth factor (TGF)-3. Interestingly, the MSCs on TCPS adhered and spread, while those on DTOPV tended to form aggregates within several days. The cells cultured on DTOPV for two weeks had a round morphology, with stronger Safranine O staining of the extracellular matrix than that of the cells cultured on TCPS. Also, Type II collagen gene was significantly expressed in cells induced on DTOPV. These results indicate that chondrogenic differentiation of MSCs proceeds more rapidly on DTOPV than on TCPS. Therefore, in cartilage tissue engineering, DTOPV could be used to induce effective chondrogenic differentiation of MSCs.
참고문헌 (17)
- Caplan Al, "The mesengenic process", Clin Plast Surg, 21, pp.429~435 (1994)
- Cho H, "Highly fluorescent and photochromic diarylethene oligomer bridged by p-phenylenevinylene", Macromolecules, 35, pp.8684~8687 (2002)
- 조혜경, "인간 지방조직에서 분리된 줄기세포의 표면항원 및 다분화능 확인", 대한임상검사과학회지, 40(2), pp.106~112 (2008), 대한임상검사과학회
- Djouad F, "Engineered mesenchymal stem cells for cartilage repair", Regen Med, 1, pp.529~537 (2006)
- Friedenstein AJ, "Osteogenesis in transplants of bone marrow cells", J Embryol Exp Morphol, 16, pp.381~390 (1966)
- Jiang Y, "Pluripotency of mesenchymal stem cells derived from adult marrow", Nature, 418, pp.41~49 (2002)
- Jiao YP, "Surface modification of polyester biomaterials for tissue engineering", Biomed Mater, 2, pp.R24~R37 (2007)
- 김한수, "Fast and Efficient Isolation of Mouse Bone Marrow-Derived Mesenchymal Stem Cells by Using a Biocompatible Polymer", 조직공학과 재생의학, 7(4), pp.443~451 (2010), 한국조직공학과 재생의학회
- Michel G, "The cell wall polysaccharide metabolism of the brown alga Ectocarpus siliculosus. Insights into the evolution of extracellular matrix polysaccharides in Eukaryotes", New Phytol, 188, pp.82~97 (2010)
- Mitchell SA, "Improved cellular adhesion to acetone plasma modified polystyrene surfaces", J Colloid Interface Sci, 1, pp.122~129 (2005)
- Shin H, "Biomimetic materials for tissue engineering", Biomaterials, 24, pp.4353~4364 (2003)
- Stevens MM, "Exploring and engineering the cell surface interface", Science, 310, pp.1135~1138 (2005)
- Wang W, "3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells", Biomaterials, 30, pp.2705~2715 (2009)
- Yoo J, "Multistate fluorescence switching of s-triazine-bridged p-phenylene vinylene polymers", Appl Phys Lett, 91, pp.241107-1~241107-3 (2007)
- You J, "A fluorescent polymer for patterning of mesenchymal stem cells", Macromolecules, 42, pp.3326~3332 (2009)
- You J, "Impact of nanotopography, heparin hydrogel microstructures, and encapsulated fibroblasts on phenotype of primary hepatocytes", ACS Appl Mater Interfaces, 7(23), pp.12299~12308 (2015)
- You J, "Multilayered heparin hydrogel microwells for cultivation of primary hepatocytes", Adv Healthc Mater, 3, pp.126~132 (2014)