Morphology of CD4+ T Lymphocytes Bound on Nano-Patterened Substrates for Sensing the Size of Nanoholes
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We report on direct finding of how the morphology (i.e. filopodia width) of CD4+ T lymphocytes correlates with the size of the quartz nanohohole arrays (QNHAs, 140, 200, 270, and 550 nm in diameter) via scanning electron microscopy (SEM). This research exhibits that the filopodia of CD4+ T-lymphocytes extended on the QNHA substrates were observed to increase in width by increasing the size of QNHA in diameter from 140 to 550 nm. This strong linear response (R2=0.988, n = 6) in filopodia’s width of surface-bound CD4+ Tcells with topographical structures of QNHA can be explained by contact guidance between the cells and solid-state substrates. Furthermore, this research suggests that the protruded filopodia of surface-bound T-lymphocytes can be used as a biosensor for sensing the topographical information of the nano-patterned substrates.
Abstract
We report on direct finding of how the morphology (i.e. filopodia width) of CD4+ T lymphocytes correlates with the size of the quartz nanohohole arrays (QNHAs, 140, 200, 270, and 550 nm in diameter) via scanning electron microscopy (SEM). This research exhibits that the filopodia of CD4+ T-lymphocytes extended on the QNHA substrates were observed to increase in width by increasing the size of QNHA in diameter from 140 to 550 nm. This strong linear response (R2=0.988, n = 6) in filopodia’s width of surface-bound CD4+ Tcells with topographical structures of QNHA can be explained by contact guidance between the cells and solid-state substrates. Furthermore, this research suggests that the protruded filopodia of surface-bound T-lymphocytes can be used as a biosensor for sensing the topographical information of the nano-patterned substrates.
참고문헌 (19)
- J. M. Lorcy, "Coaxial nickel", poly(p-phenylene vinylene) nanowires as luminescent building blocks manipulated magnetically, 2009, pp.20(40), 405601~
- S. Schafer, "Laser-induced charge separation in CdSe nanowires", Nano Lett, 11(7), pp.2672~2677 (2011)
- P. D. Yang, "Semiconductor nanowire: What's next?", Nano Lett, 10(5), pp.1529~1536 (2010)
- K. S. Brammer, "Hydrophobic nanopillars initiate mesenchymal stem cell aggregation and osteo-differentiation", Acta Biomater, 7(2), pp.683~690 (2011)
- W. Kim, "Interfacing silicon nanowires with mammalian cells", J. Am. Chem. Soc, 129(23), pp.7228~7229 (2007)
- Jianping Fu, "Mechanical regulation of cell function with geometrically modulated elastomeric substrates", Nat. Methods, 7(9), pp.799~ (2010)
- S. T. Kim, "Novel streptavidin-functionalized silicon nanowire arrays for CD4(+) T lymphocyte separation", Nano Lett, 10(8), pp.2877~2883 (2010)
- S. K. Lee, "Nanowire substrate-based laser scanning cytometry for quantitation of circulating tumor cells", Nano Lett, 12(6), pp.2697~2704 (2012)
- D. J. Kim, "A quartz nanopillar hemocytometer for high-yield separation and counting of CD4(+) T lymphocytes", Nanoscale, 4(7), pp.2500~2507 (2012)
- Anselme K, "The interaction of cell and bacteria with surfaces structured at the nanometre scale", Acta Biomater, 6, pp.3824~ (2010)
- Xuanhong Cheng, "Enhancing the performance of a point-of-care CD4+ T-cell counting microchip through monocyte depletion for HIV", AIDS diagnosis, 2009, pp.9(10), 1357~1364
- 박래경, "전이성 유방암 환자에서 혈청 HER-2", neu 와 CA15-3의 상관성, 2008, pp.11(1), 18~24[DOI]
- Yair Fisher, "The correlation of serum HER-2", neu and CA 15-3 in patients with plaques enhance plaque clearance in a mouse model of Alzheimer’s disease, 2010, pp.5(5), e10830~
- D. J. Kim, "Cell adhesion and migration on nanopatterned substrates and their effects on cell-capture yield", Nanotechnology, 23, pp.395102~ (2012)
- C. J. Bettinger, "Engineering substrate topography at the micro- and nanoscale to control cell function", Angew. Chem. Int. Edit, 48(30), pp.5406~5415 (2009)
- M. J. Dalby, "Topographically induced direct cell mechanotransduction", Med. Eng. Phy, 27(9), pp.730~742 (2005)
- M. J. Dalby, "Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size", Int. J. Biochem. Cell B, 36(10), pp.2005~2015 (2004)
- A. Hart, "Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography", J. Mater. Sci.-Mater. M, 18(6), pp.1211~1218 (2007)
- M. J. Dalby, "Polymer-demixed nanotopography: Control of fibroblast spreading and proliferation", Tissue Eng, 8(6), pp.1099~1108 (2002)