Effects of nanoscale ridge/groovepattern arrayed surface on in vitro differentiation of multi-potent pulp cells derived from human supernumerary teeth
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of thetissues that differentiate from these cells can provide insights for future regenerative therapeutics and tissue engineering strategies. The mesiodens is the most frequent form of supernumerary tooth from which DPSCs can differentiate into several lineages similar to cells from normal deciduous teeth. Recently, it has been shown that nanoscale structures can affect stem cell differentiation. In our presentstudy, we investigated the effects of a 250-nm nanoscale ridge/groove pattern array on the osteogenic and adipogenic differentiation of dental pulp cells from mesiodenscontaining human DPSCs. To this end,the expression of lineage specific markers after differentiation induction was analyzed by lineage specific staining and RT-PCR. The nanoscale pattern arrayed surface showed apositive effect on the adipogenic differentiation of DPSCs. There was no difference between nanoscale pattern arrayed surface and conventional surface groups onosteogenic differentiation. In conclusion, the nanoscale ridge/groove pattern arrayed surface can be used to enhance the adipogenic differentiation of DPSCs derived from mesiodens. This finding provides an improved understanding of the effects of topography on cell differentiation as well as the potential use of supernumerary tooth in regenerative dental medicine.
Abstract
Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of thetissues that differentiate from these cells can provide insights for future regenerative therapeutics and tissue engineering strategies. The mesiodens is the most frequent form of supernumerary tooth from which DPSCs can differentiate into several lineages similar to cells from normal deciduous teeth. Recently, it has been shown that nanoscale structures can affect stem cell differentiation. In our presentstudy, we investigated the effects of a 250-nm nanoscale ridge/groove pattern array on the osteogenic and adipogenic differentiation of dental pulp cells from mesiodenscontaining human DPSCs. To this end,the expression of lineage specific markers after differentiation induction was analyzed by lineage specific staining and RT-PCR. The nanoscale pattern arrayed surface showed apositive effect on the adipogenic differentiation of DPSCs. There was no difference between nanoscale pattern arrayed surface and conventional surface groups onosteogenic differentiation. In conclusion, the nanoscale ridge/groove pattern arrayed surface can be used to enhance the adipogenic differentiation of DPSCs derived from mesiodens. This finding provides an improved understanding of the effects of topography on cell differentiation as well as the potential use of supernumerary tooth in regenerative dental medicine.
참고문헌 (23)
- Zuk PA, "Human adipose tissue is a source of multipotent stem cells", Mol Biol Cell, 13, pp.4279~4295 (2002)
- Young HE, "Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors", Anat Rec, 264, pp.51~62 (2001)
- Su EJ, "Gene therapy vector-mediated expression of insulin-like growth factors protects cardiomyocytes from apoptosis and enhances neovascularization", Am J Physiol Heart Circ Physiol, 284, pp.H1429~H1440 (2003)
- Yen BL, "Isolation of multipotent cells from human term placenta", Stem Cells, 23, pp.3~9 (2005)
- Gronthos S, "Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo", Proc Natl Acad Sci U S A, 97, pp.13625~13630 (2000)
- Gronthos S, "Stem cell properties of human dental pulp stem cells", J Dent Res, 81, pp.531~535 (2002)
- Kerkis I, "Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers", Cells Tissues Organs, 184, pp.105~116 (2007)
- Otaki S, "Mesenchymal progenitor cells in adult human dental pulp and their ability to form bone when transplanted into immunocompromised mice", Cell Biol Int, 31, pp.1191~1197 (2007)
- Nikolic N, "Mesenchymal Stem Cell Properties of Dental Pulp Cells from Deciduous Teeth", Arch Biol Sci, 63, pp.933~942 (2011)
- Huang AHC, "Isolation and characterization of dental pulp stem cells from a supernumerary tooth", J Oral Pathol Med, 37, pp.571~574 (2008)
- Jo YY, "Isolation and characterization of postnatal stem cells from human dental tissues", Tissue Eng, 13, pp.767~773 (2007)
- Dupont S, "Role of YAP", TAZ in mechanotransduction, 2011, pp.474, 179~183
- Rossier OM, "Force generated by actomyosin contraction builds bridges between adhesive contacts", EMBO J, 29, pp.1055~1068 (2010)
- Lombardi ML, "The Interaction between Nesprins and Sun Proteins at the Nuclear Envelope Is Critical for Force Transmission between the Nucleus and Cytoskeleton", J Biol Chem, 286, pp.26743~26753 (2011)
- Doyle AD, "One-dimensional topography underlies three-dimensional fibrillar cell migration", J Cell Biol, 184, pp.481~490 (2009)
- Karaöz E, "Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells", Histochem Cell Biol, 136, pp.455~473 (2011)
- Lee MR, "Direct differentiation of human embryonic stem cells into selective neurons on nanoscale ridge", groove pattern arrays, 2010, pp.31, 4360~4366
- Niemelä S, "Adipose tissue and adipocyte differentiation: Molecular and cellular aspects and tissue engineering applications", Topics in Tissue Engineering, 4, pp.1~26 (2008)
- Nam H, "Identification of novel epithelial stem cell-like cells in human deciduous dental pulp", Biochem Biophys Res Commun, 386, pp.135~139 (2009)
- Coppe C, "Characterization of primary dental pulp cells in vitro", Pediatr Dent, 31, pp.467~471 (2009)
- Csaki C, "Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study", Histochem Cell Biol, 128, pp.507~520 (2007)
- Beresford JN, "Evidence for an Inverse Relationship between the Differentiation of Adipocytic and Osteogenic Cells in Rat Marrow Stromal Cell-Cultures", J Cell Sci, 102, pp.341~351 (1992)
- Justesen J, "Adipocyte tissue volume in bone marrow is increased with aging and in patients with osteoporosis", Biogerontology, 2, pp.165~171 (2001)