Simple Formation of Poly(sodium 4-styrenesulfonate) Pattern on the Hydrophobic Substrate for the Control of Cell Adhesion via a Selective Ion Irradiation
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, the simple preparation of poly(sodium 4-styrenesulfonate) (PSS)-patternedsubstrate via a selective ion irradiation was investigated to manipulate cell adhesion. PSS thin filmsspin-coated onto the hydrophobic polystyrene (PS) was patterned through masked 150 keV protonirradiation followed by developing with deionized water. The characteristics of the resulting PSSpatternedsurfaces were investigated by using microscope, surface profiler, FT-IR, XPS, and contactangle analyzer. These analytical results revealed that the resolved 100 μm PSS patterns wereformed on the hydrophobic PS surface above the fluence of 1×1015 ions cm-2 and the chemicalstructure, composition, and wettability of the PSS patterns were dependant on a fluence. Moreover,the results of the in-vitro cell culture and proliferation assay exhibited that H1299 cells preferentiallyadhered and proliferated onto the more hydrophilic PSS part of the PSS-patterned PSand the well-aligned cell patterns was formed on the PSS-patterned PS particularly at the fluenceof 1×1015 ions cm-2.
Abstract
In this study, the simple preparation of poly(sodium 4-styrenesulfonate) (PSS)-patternedsubstrate via a selective ion irradiation was investigated to manipulate cell adhesion. PSS thin filmsspin-coated onto the hydrophobic polystyrene (PS) was patterned through masked 150 keV protonirradiation followed by developing with deionized water. The characteristics of the resulting PSSpatternedsurfaces were investigated by using microscope, surface profiler, FT-IR, XPS, and contactangle analyzer. These analytical results revealed that the resolved 100 μm PSS patterns wereformed on the hydrophobic PS surface above the fluence of 1×1015 ions cm-2 and the chemicalstructure, composition, and wettability of the PSS patterns were dependant on a fluence. Moreover,the results of the in-vitro cell culture and proliferation assay exhibited that H1299 cells preferentiallyadhered and proliferated onto the more hydrophilic PSS part of the PSS-patterned PSand the well-aligned cell patterns was formed on the PSS-patterned PS particularly at the fluenceof 1×1015 ions cm-2.
참고문헌 (23)
- Mi-Young Ahn, "Cell patterning on a poly(N-vinyl pyrrolidone)-patterned polystyrene substrate by using ion implantation", Journal of Industrial and Engineering Chemistry, 16(1), pp.87~90 (2010), 한국공업화학회
- Arnold M, "Cell interactions with hierarchically structured nano-patterned adhesive surfaces", Soft Matte, 5, pp.72~77 (2009)
- Chiang CC, "Optical tweezers based active microrheology of sodium polystyrene sulfonate (NaPSS)", Opt. Express, 19, pp.8847~8854 (2011)
- Chien HW, "Spatial control of cellular adhesion using photo-crosslinked micropatterned polyelectrolyte multilayer films", Biomaterials, 30, pp.2209~2218 (2009)
- Dalby MJ, "The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography", J. Biomed. Mater. Res. A, 84A, pp.973~979 (2008)
- Dong H, "State-of-the-art overview : ion beam surface modification of polymers towards improving tribological properties", Surf. Coat. Technol, 111, pp.29~40 (1999)
- Garg S, "Development and characterization of bioadhesive vaginal films of sodium polystyrene sulfonate(PSS), a novel contraceptive antimicrobial agent", Pharm. Res, 22, pp.584~595 (2005)
- Gong YH, "Photoresponsive smart template for reversible cell micropatterning", J. Mater. Chem. B, 1, pp.2013~2017 (2013)
- Guillotin B, "Cell patterning technologies for organotypic tissue fabrication", Trends Biotechnol, 29, pp.183~190 (2011)
- Hasebe T, "Tailoring surface topographies of polymers by using ion beam : Recent advances and the potential applications in biomedical and tissue engineering", Nucl. Instrum. Meth. B, 282, pp.134~136 (2012)
- Hwang IT, "Micropatterning of mammalian cells on indium tin oxide substrates using ion implantation", J. Biomed. Nanotechnol, 9, pp.819~824 (2013)
- Hwang IT, "Simple and biocompatible micropatterning of multiple cell types on a polymer substrate by using ion implantation", Langmuir, 26, pp.18437~18441 (2010)
- Jung CH, "Patterning of cells on a PVC film surface functionalized by ion irradiation", Polym. Adv. Technol, 21, pp.135~138 (2010)
- Kondyurin A, "Argon plasma immersion in implantation of polystyrene films", Nucl. Instrum. Meth. B, 266, pp.1074~1084 (2008)
- Nakanishi J, "Recent advances in cell micropatterning techniques for bioanalytical and biomedical sciences", Anal. Sci, 24, pp.67~72 (2008)
- Park JW, "Dual ion beam irradiation of polymeric materials for the modification of optical properties with improved adhesion", Nucl. Instrum. Meth. B, 281, pp.51~55 (2012)
- Robertus J, "Dynamic control over cell adhesive properties using molecular-based surface engineering strategies", Chem. Soc. Rev, 39, pp.354~378 (2010)
- Sommani P, "Line-width of ion beam-modified polystyrene by negative carbon ions for fine adhesion pattern of mesenchymal stem cells", Surf. Coat. Technol, 206, pp.897~899 (2011)
- Suzuki Y, "Surface modification of polystyrene for improving wettability by ion implantation", Nucl. Instrum. Meth. B., 80, pp.81 (1993), 1067~1071
- Thery M, "Micropatterning as a tool to decipher cell morphogenesis and functions", J. Cell Sci, 123, pp.4201~4213 (2010)
- Versaevel M, "Spatial coordination between cell and nuclear shape within micropatterned endothelial cells", Nat. Commun, 3, pp.671~ (2012)
- Wang YY, "Introducing RGD peptides on PHBV films through PEG-containing cross-linkers to improve the biocompatibility", Biomacromolecules, 12, pp.551~559 (2011)
- Yang JC, "NMR and FT-IR studies of sulfonated styrene-based homopolymers and copolymers", Polymer, 43, pp.5125~5132 (2002)