미성숙 화기를 이용한 ‘우람’ 억새 식물체 재분화
Plant Regeneration of M. sacchariflorus cv. Wooram Using Immature Inflorescence
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초록
본 연구는 한국형 바이오에너지작물로 선발된 물억새인‘우람’의 미성숙 화기를 이용한 캘러스 유도 및 식물체 재분화 조건을 확립하고자 하였다. 캘러스 유도율은 MS배지에2,4-D 3 mg L-1 첨가에서 93.3%로 높게 나타났으며, 2,4-D 3 mg L-1과 BA 0.1 mg L-1 처리에서 86.7%로 높게 나타났다. 식물체 재분화율은 BA가 첨가된 배지에서 유도된 캘러스를 이용하였을 경우 BA가 첨가되지 않은 배지에서 유도된 캘러스보다 높게 나타나는 것을 확인하였다. 재분화를 위한 배지 조성은 BA 5 mg L-1과 NAA 0.1 mg L-1 호르몬 조합에서 재분화율이 86.7%로 가장 효과적이었다. 재분화된 소식물체는 캘러스로부터 분리하여 MS배지에서 발근을 유도하였을때 배양 4주 이후 뿌리가 3 cm 이상 신장하였다. 기내에서 발근이 된 소식물체의 순화를 위해 상토에 이식하고 온실에서재배하였을 때, 5주 후 지상부가 20 cm 이상으로 신장하여성공적으로 활착되었다.
Abstract
This study was carried out to establish the optimal conditions for callus induction and plant regeneration using immature inflorescence of M. sacchariflorus cv. ‘Wooram’, a bioenergy crop selected in Korea. Callus induction rate was the highest (93.3%) in MS medium containing 3 mg L-1 2,4-D, and 86.7% in MS medium containing 3 mg L-1 2,4-D combined with 0.1 mg L-1 BA. Plant regeneration rate was high when the calli derived from the medium containing BA was used, as compared with those derived from the BA-minus medium. The results showed that the medium conditions containing 5 mg L-1 BA combined with 0.1 mg L-1 NAA was the most effective in plant regeneration of which the rate reached 86.7%. The regenerated shoots were separated from the calli and roots over 3 cm were developed from the shoots after 4 week culture on basal MS medium without supplementation. The plantlets were then transferred to soil and cultured in greenhouse. After 5 weeks, the plants with the height of at least 20 cm were successfully acclimatized.
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