켄터키 블루그래스에 있어서 캘러스 배양 및 식물체 재분화에 미치는 요인의 영향
Factors Affecting Callus Culture and Plant Regeneration in Kentucky Bluegrass
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켄터키 블루그래스의 최적 조직배양조건을 확립하기 위하여 성숙종자로부터 배발생 캘러스 유도 및 효율적인 식물체 재분화를 위한 품종별 배양효과, 배지종류 및 배지첨가물 등의 최적조건을 조사하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 3mg/L 2,4-D와 0.1mg/L BA가 첨가된 MS배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1mg/L 2,4-D와 3mg/L BA가 첨가된 N6 배지에서 배양했을 때 57.7%의 높은 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스 유도에는 MS 배지가, 식물체 재분화에는 N6 배지가 효과적이었다. 켄터키 블루그래스의 품종에 따른 배발생 캘러스의 유도율과 재분화 능력은 큰 차이를 나타내어 Newport가 가장 높은 배양효율을 나타내었다. 본 연구를 통하여 확립된 단기간의 효율적인 재분화시스템은 분자육종을 통한 신품종 켄터키 블루그래스 개발에 유용하게 응용되어질 수 있을 것이다.
Abstract
In order to optimize tissue culture conditions of Kentucky bluegrass(Poa pratensis L.), effects of culture medium supplements, media and cultivars on embryogenic callus induction and regeneration of plants were investigated. MS medium containing 3mg/L 2,4-D and 0.1mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency(57.7%) was observed when the embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Newport’ showed to have higher regeneration frequency of 53.4%. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of Kentucky bluegrass through genetic transformation.
참고문헌 (20)
- "Rapid production of transgenic turfgrass", 157, pp.441~448 (2000)
- "An evaluation on callus induction and plant regeneration of 25 turf-type tall fescue", 55, pp.326~330 (2000)
- "Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources", pp.659~668 (1975)
- "Forage and turf grass biotechnology: principles, methods and prospects.", 21, pp.181~ (1999), Kluwer Academic Publishers,
- "Plant regeneration in Kentucky bluegrass via coleoptile tissue cultures", 15, pp.882~887 (1996)
- "Cell culture of turfgrass", pp.27~33 (1981)
- "High-frequency plant regeneration from mature seed-derived callus culture of orchardgrass", 30, pp.341~346 (2003)
- "Improving forage production systems using biotechnolgy.", (17), pp.3~ (1997)
- "A revise medium for rapid growth and bioassays with tobacco tissue cultures", pp.473~497 (1962)
- "Regeneration of green plants from embryogenic suspension culture of Kentucky bluegrass", pp.537~540 (1993)
- "Callus induction from seeds of Italian ryegrass and plant regeneration", 20, pp.25~0 (2000)
- "SAS", STAT Software for PC, 1999, pp.SAS institute,, ~
- "Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures"
- "Biotechnology in forage and turf grass improvement", 23 (1998), Heidelberg, p
- "Impro- vement of tissue culture response of seed-derived callus cultures of Kentucky bluegrass", pp.275~280 (1991), Creemers- Molenaar
- "Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass the effect of benzyladenine", 40, pp.101~103 (1995)
- "Achievements and prospectives in the breeding of forage grasses and legumes Grasslands for our world" (1993)
- "Induction and maintenance of embryogenic callus cultures of Gramineae Cell culture and somatic cell genetics of plants", 1, pp.36~42 (1984), Academic Press
- "Plants from cell suspension-derived protoplasts in Lolium species", pp.179~193 (1993)
- "plants from microprojectile bombardment of embryogenic suspension cells", pp.379~384 (1997)