이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화
Efficient Plant Regeneration from Mature Seed-derived Callus of Italian ryegrass (Lolium multiflorum Lam.)
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이탈리안 라이그래스의 최적 조직배양 조건을 확립하기 위 하여 성숙종자로부터 최적 배발생 캘러스 유도조건 및 효율 적인 식물체 재분화 체계를 확립하였다. 배발생 캘러스 유도 시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 5 mg/L 2,4-D가 첨가된 MS배지에서 배발생 캘러스가 가장 높 은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1mg/L 2,4-D와 5 mg/L BA가 첨가된 N6배지에서 배양했을 때 70% 이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스 유도에는 MS배지가, 식물체 재분 화에는 N6배지가 효과적이었다. 배발생 캘러스의 형성율은 품종 간에 큰 차이가 없었으나, 재분화 능력은 Jeanne 품종이 73%의 가장 높은 재분화율을 나타내어 품종 간에 큰 차이를 나타내었다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 이탈리안 라이그래스의 개 발에 유용하게 응용되어질 수 있을 것이다.
Abstract
As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass(Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plantregeneration were evaluated. MS medium containing 5 mg/L 2,4-D was optimal for embryogenic callus inductionfrom mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency wasobserved at above 70% when embryogenic calli induced were transferred to N6 medium supplemented with 1 mg/L2,4-D and 5 mg/L BA. Among several basic media tested, MS and N6 medium were optimal for callus inductionand plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Jeanne’showed thehighest regeneration frequency of 73%. A short tissue culture period and high-frequency regeneration systemestablished in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.
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