억새(Miscanthus sinensis) 종자로부터 캘러스 유도를 통한 식물체 재생에 영향을 미치는 요인
Factors Affecting Plant Regeneration via Callus Induction from Seeds of Miscanthus sinensis
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Plant regeneration protocols via callus induction from seeds of Miscanthus sinensis, a promising bioenergy crop, were developed. Basal media and 2,4-D concentrations, proline concentrations, and kinds and concentrations of gelling agent were tested to find out the optimal conditions for callus induction from mature seeds. On the combination treatment of basal media,MS medium and N6 medium, and 2,4-D concentrations, callus induction rate was the highest (42.2%) in MS medium with 4.0mg/L 2,4-D. Proline improved callus formation from seeds, and high concentration (12.0 g/L) of proline was good for callus induction. In gelling agent treatment, Phytagel was better for callus formation than agar and 2.25 g/L Phytagel produced the highest (86.1%) callus induction. Calli obtained from seeds were subcultured every 4 weeks. After 3 months of callus induction,calli with dense yellow color and dome shape were selected and used for plant regeneration. The callus, cultured on hormone-free MS medium or MS medium with 1.0 mg/L BA, produced adventitious shoot. The regenerated shoots were elongated and rooted on MS medium. The plantlets were acclimatized successfully and the regenerated plants showed normal phenotypes.
Abstract
Plant regeneration protocols via callus induction from seeds of Miscanthus sinensis, a promising bioenergy crop, were developed. Basal media and 2,4-D concentrations, proline concentrations, and kinds and concentrations of gelling agent were tested to find out the optimal conditions for callus induction from mature seeds. On the combination treatment of basal media,MS medium and N6 medium, and 2,4-D concentrations, callus induction rate was the highest (42.2%) in MS medium with 4.0mg/L 2,4-D. Proline improved callus formation from seeds, and high concentration (12.0 g/L) of proline was good for callus induction. In gelling agent treatment, Phytagel was better for callus formation than agar and 2.25 g/L Phytagel produced the highest (86.1%) callus induction. Calli obtained from seeds were subcultured every 4 weeks. After 3 months of callus induction,calli with dense yellow color and dome shape were selected and used for plant regeneration. The callus, cultured on hormone-free MS medium or MS medium with 1.0 mg/L BA, produced adventitious shoot. The regenerated shoots were elongated and rooted on MS medium. The plantlets were acclimatized successfully and the regenerated plants showed normal phenotypes.
참고문헌 (26)
- Armstrong CL, "Regeneration of plants from somatic cell cultures: applications for in vitro genetic manipulation, In The Maize Handbook", pp.663~671 (1994), Springer Verlag
- Armstrong CL, "Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline", Planta, 164, pp.207~214 (1985)
- Beale CV, "Leaf photosynthesis in the C4-grass Miscanthus giganteus, growing in the cool temperate climate of southern England", J. Exp. Bot, 47, pp.267~273 (1996)
- Chris S, "Feedstocks for lignocellulosic biofuels", Science, 329, pp.790~792 (2010)
- Chu CC, "Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources", Sci. Sin, 18, pp.659~668 (1975)
- 정재훈, "수송용 바이오에너지 개발과 미래", 한국미생물·생명공학회지, 36(1), pp.1~5 (2008), 한국미생물·생명공학회
- Fitch MMM, "Long-term culture of embryogenic sugarcane callus", Plant Cell Tiss. Org. Cult, 32, pp.335~343 (1993)
- Głowacka K, "The effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species", Plant Cell Tiss. Org. Cult, 102, pp.79~86 (2010)
- Harn C, "Transition in the plant breeding technology", Kor. J. Breed. Sci, 23, pp.68~72 (1991)
- Holme IB, "Embryogenic callus formation, growth and regeneration in callus and suspension cultures of Miscanthus × ogiformis Honda‘Giganteus’ as affected by proline", Plant Cell Tiss. Org. Cult, 50, pp.203~210 (1997)
- Holme IB, "Callus induction and plant regeneration from different explant types of Miscanthus x ogiformis Honda ‘Giganteus’", Plant Cell Tiss. Org. Cult, 45, pp.43~52 (1996)
- Kim HS, "Miscanthus× giganteus plant regeneration: effect of callus types, ages and culture methods on regeneration competence", GCB Bioenergy, 2, pp.192~200 (2010)
- Lewandowski I, "Miscanthus：European experience with a novel energy crop", Biomass Bioenergy, 19, pp.209~227 (2000)
- Lewandowski I, "Nitrogen, energy and land use efficiencies of miscanthus, reed canary grass and triticale as determined by the boundary line approach", Agric. Ecosyst. Environ, 112, pp.335~346 (2006)
- Luo JP, "Callus induction and plant regeneration from hypocotyl explants of the forage legume Astragalus adsurgens", Plant Cell Rep, 17, pp.567~570 (1998)
- 문윤호, "유망 바이오에너지작물 “억새” 개발", Weed&Turfgrass Science, 30(4), pp.330~339 (2010), 한국잡초학회
- Morrish F, "Developmental morphogenesis and genetic manipulation in tissue and cell cultures of the Gramineae", Adv. Genet, 24, pp.431~499 (1987)
- Murashige T, "A revised medium for rapid growth and bio assays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- Pareddy DR, "Somatic embryogenesis and plant regeneration from immature inflorescences of several elite inbreds of maize", Plant Sci, 67, pp.211~219 (1990)
- 박충훈, "억새 성숙종자로부터 캘러스 유도 및 식물체 재분화에 있어서 식물생장호르몬의 영향", 한국초지조사료학회지, 29(4), pp.291~298 (2009), 한국초지조사료학회
- Petersen KK, "Callus induction and plant regeneration in Miscanthus x ogiformis Honda ‘Giganteus’ as influenced by benzyladenine", Plant Cell Tiss. Org. Cult, 49, pp.137~140 (1997)
- Petersen KK, "Significance of different carbon sources and sterilization methods on callus induction and plant regeneration of Miscanthus x ogiformis Honda ‘Giganteus’", Plant Cell Tiss. Org. Cult, 58, pp.189~197 (1999)
- Suprasanna P, "Embryogenic callus in maize: Genotypic and amino acid effects", Cereal Res. Commun, 22, pp.79~82 (1994)
- 이효연, "Production of Herbicide-tolerant Zoysiagrass by Agrobacterium-mediated Transformation", Molecules and Cells, 16(1), pp.19~27 (2003), 한국분자세포생물학회
- Vasil V, "Plant regeneration from friable embryogenic callus and cell suspension cultures of Zea mays L", J. Plant Physiol, 124, pp.399~408 (1986)
- Wang H, "Effect of four brands of gelling agents on callus induction from mature seeds of several turfgrass species", Acta Hort. Sin, 33, pp.437~440 (2006)