바이오에너지용 수수 품종의 재분화율 증진을 위한 배지와 생장조절제 효과
Effect of Plant Growth Regulators and Media on Regeneration of Sorghum bicolor (L.) Moench
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초록
본 연구는 수수의 성숙 종자로부터 캘러스유기 및 재분화 효율 조사하기위하여 수행되었다. 배발생 캘러스 형성을 살펴보기 위해 생장조절제 효과를 조사하였다. 수수 성숙 종자로부터 캘러스 유기에 적합한 생장조절제 종류와 농도는 2 ㎎/L 2,4-D인 것으로 나타났다. 배발생 캘러스의 고효율 빈도는 B5 배지에 2 ㎎/L 2,4-D을 첨가하였을 때 가장 양호한 것으로 나타났다. 배발생 캘러스로부터 식물체 재분화는 BAP와 IBA를 넣은 MS 배지가 가장 적절한 것으로 나타났다. BAP 농도가 높을수록 신초재분화가 활발하였다. 따라서, 본 연구 결과로부터 수수 기내배양시 신초와 발근 형성에 효과적인 생장조절제 조합은 0.5 mg l^(-1) BAP와 0.25 mg l^(-1) IBA인 것으로 나타났다.
Abstract
This study was carried out to optimize the embryogenic callus induction and plant regeneration from mature seeds of Sorghum bicolor. The effect of growth regulators was investigated on formation of embryogenic callus. The highest frequency of embryogenic callus was observed when the mature seeds were cultured on B5 medium supplemented with 2 ㎎/L 2,4-Dichlorophenoxyacetic acid (2,4-D). The highest frequency of plant regeneration from embryogenic callus was observed on MS medium with 0.5 mg l-1 6-benzyl amino purine (BAP) and 0.25 mg l-1 indole-3-butyric acid (IBA) to optimize the shoot regeneration. High concentration of BAP (1 mg l-1) supplemented with IBA (0.25 mg l-1) was effective combination for shoot multiplication. MS medium supplemented with 1 mg l-1 IBA was found to be the most effective for inducing roots. Normal rooted plantlets were transferred to the greenhouse for hardening with over 90% survival rate. Hence, this reproducible protocol could be useful for mass propagation and genetic transformation of S. bicolor.
참고문헌 (27)
- Arlene, H., "Rapid and reproducible Agrobacterium-mediated transformation of sorghum", Plant Cell Rep, 25, pp.284~291 (2006)
- Bai, Z. L., "A study on the callus induction and plant regeneration of different sorghum explants", Acta Agriculturae Boreali Sinica, 10, pp.60~63 (1995)
- P. Baskaran, "An Efficient Plant Regeneration System for Sorghum bicolor-a Valuable Major Cereal Crop", Journal of Plant Biotechnology, 7(4), pp.247~257 (2005), 한국식물생명공학회
- Bhat, S., "Callus induction and plant regeneration from immature embryos of maintainer line (B) of kharif sorghum", J. Maharashtra Agricult. University, 20, pp.159~ (1995)
- Cai, T., "Plant regeneration from embryogenic callus initiated from immature inflorescence of several hightannin sorghums", Plant Cell Tiss. Org. Cult, 20, pp.101~110 (1990)
- 조남기, "제주 재래수수의 재식밀도에 따른 생육, 수량 및 사료가치에 미치는 영향.", 한국초지학회지, 24(3), pp.225~230 (2004), 한국초지조사료학회
- Chu, C. C., "Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources", Scientia Sinic, 18, pp.659~668 (1975)
- Elkonin, L. A., "Influence of nitrogen sources on induction and growth of embryogenic callus in sorghum", Inter. Sorghum and Millets Newsletter, 37, pp.68~69 (1996)
- Gamborg, O. L., "Nutrient requirements of suspension cultures of soybean root cells", Exp. Cell Res, 50, pp.151~158 (1968)
- Gupta, S., "Strategies for overcoming genotypic limitations of in vitro regeneration and determination of genetic components of variability of plant regeneration traits in sorghum", Plant Cell Tiss. Org. Cult, 86, pp.379~388 (2006)
- Hagio, T., "Adventitious shoot regeneration from immature embryos of sorghum", Plant Cell Tiss. Org. Cult, 68, pp.65~72 (2002)
- Han, F. G., "Screening for salt tolerant lines through in vitro culture under salt stress conditions and studies on their different characters", Acta Agron. Sinica, 23, pp.491~495 (1997)
- Jogeswar, G., "High Frequency somatic embryogenesis and regeneration in different genotypes of Sorghum bicolor (L.) Moench from immature inflorescence explants", In Vitro Cell. Develop. Biol. Plant, 43, pp.159~166 (2007)
- Kaeppler, H. F., "Evaluation of 41 elite and exotic inbred sorghum genotypes for high quality callus production", Plant Cell Tiss. Org. Cult, 48, pp.71~75 (1997)
- Maheswari, M., "Efficient plant regeneration from shoot apices of sorghum", Plant Biol, 50, pp.741~744 (2006)
- Mamun, M. A., "In vitro micropropagation of some important sugarcane varieties of Bangladesh", Asian J. Plant Sci, 3, pp.666~669 (2004)
- Murashige, T., "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- Nahadi, S., "In vitro regeneration of Sorghum bicolor lines from shoot apexes", Inter. Sorghum and Millets Newsletter, 36, pp.88~90 (1995)
- Nguyen, Tuong-Van, "Agrobacterium-mediated transformation of sorghum (Sorghum bicolor (L.) Moench) using an improved in vitro regeneration system", Plant Cell Tiss. Org. Cult, 91, pp.155~164 (2007)
- Oldach, K. H., "Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennistum Glaucum (L.)R", Br.J. and Sorghum bicolor (L.) Moench. Plant Cell Rep, 20, pp.416~421 (2001)
- O’ennedy, M. M., "Harnessing sorghum and millet biotechnology for food and health", J. Cereal Sci, 44, pp.224~235 (2006)
- Patil, V. M., "Plant regeneration from leaf sheath cultures of some rabi sorghum cultivars", South African J. Bot, 64, pp.217~219 (1998)
- Rao, K. V., "Somatic embryogenesis from immature glume calli of Zea mays L", Indian J. Exp. Biol, 28, pp.531~533 (1990)
- Shyamala, D., "Efficient regeneration of sorghum, Sorghum bicolor (L.) Moench, from shoot-tip explant", Indian J. Exp. Biol, 41, pp.1482~1486 (2003)
- Vasil, V., "Somatic embryogenesis and plant regeneration from tissue cultures of Pennisetum americanum, and P. americanum x P. purpureum hybrid", Ameri. J. Bot, 68, pp.864~872 (1981)
- Yohannes, T., "Optimization of transformation conditions and production of transgenic sorghum (Sorghum bicolor) via microparticle bombardment", Plant Cell Tiss. Org. Cult, 75, pp.1~18 (2003)
- Zhao, Z., "Agrobacterium-mediated sorghum transformation.Plant Mol.Biol.44:789-798", Plant Mol. Biol, 44, pp.789~798 (2000)