Nano bioactive HAP-nano bioresorbable β-TCP-PEG composite scaffolds and their biochemical activity for implant applications
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Recent efforts towards the treatment of bone defects and diseases focus on the development of bone scaffolds. Bioceramicsprovide strength, osteoconductivity and also imparts flexibility and resorbability. In this study, the biodegradable compositeswere fabricated using bioactive nano Hydroxyapatite (n-HAP) and bioresorbable nano β-Tricalcium phosphate (n-β-TCP)taken in 1:1 proportion. The nano composite scaffolds were synthesized using PEG (Poly Ethylene Glycol) by wet precipitationmethod. XRD (X-ray diffraction) confirms the presence of crystalline structure of n-HAP and n-β-TCP within the lattice. FESEM (Field Emission Scanning Electron Microscopy) and EDS (Energy Dispersion X-ray Spectroscopy) confirms the microporous nature and the phase purity of the composite. Further, biochemical studies were carried out using MG-63 Osteoblastcell line to evaluate their sustainability after implantation. The viability of the cells and proliferation rate is evaluated using3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) with different concentration and different incubationperiod. The ALP (Alkaline Phosphotase) test reveals that the composite favours bone regeneration through apatite layerformation. Further studies were carried out to explore the DPPH (1,1-diphenyl-2-picrylhydrazyl) activity on the compositesand results reveals that the composite have an ability to trap the free radicals in the biological surroundings. The antimicrobialstudies indicate that the composites shows no major inhibitory effects towards the most common bone affecting bacteriaStaphylococcus aureus. The studies indicate that the concentration range of the composite is ideal for bone growth and can beused as substituents in the scaffold synthesis for normal and cancerous patients.
Abstract
Recent efforts towards the treatment of bone defects and diseases focus on the development of bone scaffolds. Bioceramicsprovide strength, osteoconductivity and also imparts flexibility and resorbability. In this study, the biodegradable compositeswere fabricated using bioactive nano Hydroxyapatite (n-HAP) and bioresorbable nano β-Tricalcium phosphate (n-β-TCP)taken in 1:1 proportion. The nano composite scaffolds were synthesized using PEG (Poly Ethylene Glycol) by wet precipitationmethod. XRD (X-ray diffraction) confirms the presence of crystalline structure of n-HAP and n-β-TCP within the lattice. FESEM (Field Emission Scanning Electron Microscopy) and EDS (Energy Dispersion X-ray Spectroscopy) confirms the microporous nature and the phase purity of the composite. Further, biochemical studies were carried out using MG-63 Osteoblastcell line to evaluate their sustainability after implantation. The viability of the cells and proliferation rate is evaluated using3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) with different concentration and different incubationperiod. The ALP (Alkaline Phosphotase) test reveals that the composite favours bone regeneration through apatite layerformation. Further studies were carried out to explore the DPPH (1,1-diphenyl-2-picrylhydrazyl) activity on the compositesand results reveals that the composite have an ability to trap the free radicals in the biological surroundings. The antimicrobialstudies indicate that the composites shows no major inhibitory effects towards the most common bone affecting bacteriaStaphylococcus aureus. The studies indicate that the concentration range of the composite is ideal for bone growth and can beused as substituents in the scaffold synthesis for normal and cancerous patients.
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