Effects of powder particle size on mechanostructure and cell viability of the synthesised bioceramic bone grafts
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Hydroxyapatite (HA) based biografts were syntesized at 0.68, 1.2 and 2.4 μm powder particle size by sol gel method. The effectof powder particle sizes on morphology, mechanical properties and in vitro cell viability of the biografts were analysed. Eachcomposition at different particle sizes (0.68, 1.2 and 2.4 μm) were sintered at 1180 oC and quantitative phase analysis wascharacterized by FTIR, XRD and SEM-EDX. Mechanical properties were determined by axial compression and Vickershardness tests. The highest compresion strengths and hardness values were obtained in samples having the particle size of 0.68μm and such values were increased with decreased particle sizes. Furthermore, from in vitro Cell viability tests it wasdetermined that no toxicity was detected at 0,1 and 0,3 μM concentrations at lower powder particle sizes. However, cellviability was significantly reduced in the syntesized samples having 2.4 μm particle size at 0,3 μM concentration.
Abstract
Hydroxyapatite (HA) based biografts were syntesized at 0.68, 1.2 and 2.4 μm powder particle size by sol gel method. The effectof powder particle sizes on morphology, mechanical properties and in vitro cell viability of the biografts were analysed. Eachcomposition at different particle sizes (0.68, 1.2 and 2.4 μm) were sintered at 1180 oC and quantitative phase analysis wascharacterized by FTIR, XRD and SEM-EDX. Mechanical properties were determined by axial compression and Vickershardness tests. The highest compresion strengths and hardness values were obtained in samples having the particle size of 0.68μm and such values were increased with decreased particle sizes. Furthermore, from in vitro Cell viability tests it wasdetermined that no toxicity was detected at 0,1 and 0,3 μM concentrations at lower powder particle sizes. However, cellviability was significantly reduced in the syntesized samples having 2.4 μm particle size at 0,3 μM concentration.
참고문헌 (25)
- Bezzi G, "A novel sol-gel technique for hydroxyapatite preparation", Materials Chemistry and Physics, 78, pp.816~824 (2003)[DOI]
- LeGeroz RZ, "Biodegradation and bioresorption of calcium phosphate ceramics", Clin Mater, 14, pp.65~88 (1993)[DOI]
- Hench LL, "An introduction of bioceramics", pp.1~ (1993)
- Tancred DC, "A quantitative study of the sintering and mechanical properties of hydroxyapatite", phosphate glass composites, 1998, pp.19, 1735~1743
- Layrotte P, "Sol-gel Synthesis of Amorphous Calcium Phosphate and Sintering into Microporous Hydrozyapatite Bioceramics", J Am Ceram Soc, 81, pp.1421~ (1998)
- Li P, "Apatite formation induced by silica gel in a simulated body fluid", J Am Ceram Soc, 75, pp.2094~2097 (1992)[DOI]
- Kim W, "Mechanochemical synthesis of hydroxyapatite from Ca(OH)2-P2O5 and CaOCa(OH)2-P2O5 mixtures", J Mater Sci, 35, pp.5401~5405 (2000)[DOI]
- Tas AC, "Combustion Synthesis of Calcium Phosphate Bioceramic Powders", J Eur Ceram Soc, 20, pp.2389~2394 (2000)[DOI]
- Lopez-Macipe A, "Wet chemical synthesis of hydroxyapatite particles from nonstoichiometric solutions", Journal of Materials Synthesis and Processing, 6, pp.21~26 (1998)[DOI]
- Huang LY, "A study of the process and kinetics of electrochemical deposition and the hydrothermal synthesis of hydroxyapatite coatings", J Mater Sci Mater Med, 11, pp.667~ (2000)
- Weng WJ, "Alkoxide route for preparing hydroxyapatite and its coatings", Biomaterials, 19, pp.125~131 (1998)[DOI]
- Yoshmura M, "Hydrothermal synthesis of biocompatible whiskers", J Mater Sci, 29, pp.3399~3402 (1994)[DOI]
- Lim GK, "Nanosized hydroxyapatite powders from microemulsions and emulsions stabilized by a biodegradable surfactant", J Mater Chem, 9, pp.1635~1639 (1999)[DOI]
- Demirel M, "pH-Effect on Sinterability, Mechanical Properties and Cytotoxicity of Hydroxyapatite-Based Bone Grafts", Applied Mech and Mater, 319, pp.134~139 (2013)[DOI]
- Kim IS, "Sol-gel synthesis and characterization of nanostructured hydroxyapatite powder", Materials Science and Engineering B, 111, pp.232~236 (2004)[DOI]
- Liu DM, "Water-based sol-gel synthesis of hydroxyapatite: process development", Biomaterials, 22, pp.1721~1730 (2001)[DOI]
- Afshar A, "Some Important Factors in the Wet Precipitation Process of Hydroxyapatite", Materials & Design, 24, pp.197~202 (2003)[DOI]
- Jillavenkatesa A, "Sol-gel processing of hydroxyapatite", J Mater Sci, 33, pp.4111~4119 (1998)[DOI]
- Park J, "Biomaterials: An Introduction", pp.235~ (1992), Plenum Press
- Lopes MA, "Glass-reinforced hydroxyapatite: a comprehensive study of the effect of glass composition on the crystallography of the composite", J Biomed Mater Res, 39, pp.244~251 (1998)[DOI]
- Knowles JC, "Ceramic Industry Division Annual Convention", pp.20~23 (1993)
- Sylvester PW, "Fatty acid modulation of epidermal growth factor-induced mouse mammary epithelial cell proliferation in vitro", Exp Cell Res, 214, pp.145~153 (1994)[DOI]
- Legeros RZ, "Calcium phosphates in oral biology and medicine", Monograph in Oral Sci, 15, pp.1~201 (1991)
- Fan D, "Consequence of heattreatment on properties of dentin", J Dent Res, 74, pp.525~ (1995)
- Metsger D, "Mechanical properties of sintered hydroxyapatite and tricalcium phosphate ceramic", J Mater Sci Mater Med, 10, pp.9~17 (1999)