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In vitro and in vivo analyses of the biocompatibility of sintered TCP-HA composite screws

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Biphasic calcium phosphate (BCP), which is a β-tricalcium phosphate (TCP) and hydroxyapatite (HA) composite, is used mainly as a bone graft material. However, it has recently been used for orthopedic, oral, and maxillofacial surgeries. The use of BCP to produce an absorbable screw and plate to reconstruct fractures has been suggested. Nevertheless, further research on using BCP in the surgical field will be needed. In particular, studies on the physical properties of BCP and in vivo analysis are essential. In this study, TCP and HA were mixed at a ratio of 7:3 and heat-treated to form BCP. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and an MTT assay were conducted to evaluate the surface characteristics and physical and biological properties of each sample. In addition, BCP was used to produce a screw to be implanted into the mandible of beagles. An analysis of the physical properties of BCP confirmed the intermediate properties between TCP and HA. The cell viability was better with BCP than TCP, and significant differences were observed on days 1 and 3. Histological analysis at eight weeks after inserting the BCP screw into the mandible of beagles revealed osseointegration between the BCP screw and bone. The results showed that BCP had better mechanical properties than TCP and excellent biocompatibility, highlighting its potential clinical use. In addition, according to its processing, BCP may be used to produce screws and plates for fracture repair or as membranes and space-modeling tents for guided bone regeneration.

Abstract

Biphasic calcium phosphate (BCP), which is a β-tricalcium phosphate (TCP) and hydroxyapatite (HA) composite, is used mainly as a bone graft material. However, it has recently been used for orthopedic, oral, and maxillofacial surgeries. The use of BCP to produce an absorbable screw and plate to reconstruct fractures has been suggested. Nevertheless, further research on using BCP in the surgical field will be needed. In particular, studies on the physical properties of BCP and in vivo analysis are essential. In this study, TCP and HA were mixed at a ratio of 7:3 and heat-treated to form BCP. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and an MTT assay were conducted to evaluate the surface characteristics and physical and biological properties of each sample. In addition, BCP was used to produce a screw to be implanted into the mandible of beagles. An analysis of the physical properties of BCP confirmed the intermediate properties between TCP and HA. The cell viability was better with BCP than TCP, and significant differences were observed on days 1 and 3. Histological analysis at eight weeks after inserting the BCP screw into the mandible of beagles revealed osseointegration between the BCP screw and bone. The results showed that BCP had better mechanical properties than TCP and excellent biocompatibility, highlighting its potential clinical use. In addition, according to its processing, BCP may be used to produce screws and plates for fracture repair or as membranes and space-modeling tents for guided bone regeneration.

참고문헌 (45)

  1. Buijs GJ, "A randomized clinical trial of biodegradable and titanium fixation systems in maxillofacial surgery", J Dent Res, 91, pp.299~304 (2012)[DOI]
  2. van Bakelen NB, "Decisionmaking considerations in application of biodegradable fixation systems in maxillofacial surgery--a retrospective cohort study", J Craniomaxillofac Surg, 42, pp.417~422 (2014)[DOI]
  3. Haers PE, "Biodegradable polylactide plates and screws in orthognathic surgery : technical note", J Craniomaxillofac Surg, 26, pp.87~91 (1998)[DOI]
  4. Ray MS, "Metallic fragments on the surface of miniplates and screws before insertion", Br J Oral Maxillofac Surg, 37, pp.14~18 (1999)[DOI]
  5. Erkmen E, "Three-dimensional finite element analysis used to compare methods of fixation after sagittal split ramus osteotomy : setback surgeryposterior loading", Br J Oral Maxillofac Surg, 43, pp.97~104 (2005)[DOI]
  6. Hughes TB, "Bioabsorbable implants in the treatment of hand fractures : an update", Clin Orthop Relat Res, 445, pp.169~174 (2006)[DOI]
  7. Waris E, "Bioabsorbable fixation devices in trauma and bone surgery : current clinical standing", Expert Rev Med Devices, 1, pp.229~240 (2004)[DOI]
  8. Viljanen J, "Bone changes after experimental osteotomies fixed with absorbable self-reinforced poly-L-lactide screws or metallic screws studied by plain radiographs, quantitative computed tomography and magnetic resonance imaging", Biomaterials, 16, pp.1353~1358 (1995)[DOI]
  9. Yaszemski MJ, "Evolution of bone transplantation : molecular, cellular and tissue strategies to engineer human bone", Biomaterials, 17, pp.175~185 (1996)[DOI]
  10. Bohner M, "Calcium orthophosphates in medicine: from ceramics to calcium phosphate cements", Injury, 31(Suppl 4), pp.37~47 (2000)[DOI]
  11. Bohner M, "Resorbable biomaterials as bone graft substitutes", Mater Today, 13, pp.24~30 (2010)[DOI]
  12. Ginebra MP, "Calcium phosphate cements as bone drug delivery systems : a review", J Control Release, 113, pp.102~110 (2006)[DOI]
  13. Takahashi Y, "Osteogenic differentiation of mesenchymal stem cells in biodegradable sponges composed of gelatin and beta-tricalcium phosphate", Biomaterials, 26, pp.3587~3596 (2005)[DOI]
  14. Hing KA, "Quantification of bone ingrowth within bone-derived porous hydroxyapatite implants of varying density", J Mater Sci Mater Med, 10, pp.663~670 (1999)[DOI]
  15. Damien E, "A preliminary study on the enhancement of the osteointegration of a novel synthetic hydroxyapatite scaffold in vivo", J Biomed Mater Res A, 66, pp.241~246 (2003)[DOI]
  16. Lu J, "Relationship between bioceramics sintering and micro-particles-induced cellular damages", J Mater Sci Mater Med, 15, pp.361~365 (2004)[DOI]
  17. Daculsi G, "Biphasic calcium phosphate concept applied to artificial bone, implant coating and injectable bone substitute", Biomaterials, 19, pp.1473~1478 (1998)[DOI]
  18. Kurashina K, "Ectopic osteogenesis with biphasic ceramics of hydroxyapatite and tricalcium phosphate in rabbits", Biomaterials, 23, pp.407~412 (2002)[DOI]
  19. Arinzeh TL, "A comparative study of biphasic calcium phosphate ceramics for human mesenchymal stem-cell-induced bone formation", Biomaterials, 26, pp.3631~3638 (2005)[DOI]
  20. Fedorowicz Z, "Resorbable versus titanium plates for orthognathic surgery", Cochrane Database Syst Rev, (2), pp.CD006204~ (2007)[DOI]
  21. Frøkjaer J, "Biodegradable fixation of ankle fractures. Complications in a prospective study of 25 cases", Acta Orthop Scand, 63, pp.434~436 (1992)[DOI]
  22. Claes L, "Clinical implant materials: proceedings of the Eighth European Conference on Biomaterials", pp.260~ (1990), Elsevier
  23. Claes LE, "Clinical Materials", pp.41~46 (1992), Technik + Kommunikation VerlagsGmbH
  24. Böstman O, "Biodegradable internal fixation for malleolar fractures. A prospective randomised trial", J Bone Joint Surg Br, 69, pp.615~619 (1987)[DOI]
  25. Hirvensalo E, "Fracture fixation with biodegradable rods. Forty-one cases of severe ankle fractures", Acta Orthop Scand, 60, pp.601~606 (1989)[DOI]
  26. Böstman O, "Foreignbody reactions to fracture fixation implants of biodegradable synthetic polymers", J Bone Joint Surg Br, 72, pp.592~596 (1990)[DOI]
  27. Böstman O, "Intense granulomatous inflammatory lesions associated with absorbable internal fixation devices made of polyglycolide in ankle fractures", Clin Orthop Relat Res, 278, pp.193~199 (1992)
  28. Elgendy H, "Osteoblastlike cell(MC3T3-E1)proliferation on bioerodible polymers : an approach towards the development of a bonebioerodible polymer composite material", Biomaterials, 14, pp.263~269 (1993)[DOI]
  29. Ignjatović N, "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate", poly-dl-lactide-co-glycolide biocomposites and different phosphate materials, 2006, pp.69, 976~982
  30. Durucan C, "Calcium-deficient hydroxyapatite-PLGA composites : mechanical and microstructural investigation", J Biomed Mater Res, 51, pp.726~734 (2000)[DOI]
  31. Higashi S, "Polymer-hydroxyapatite composites for biodegradable bone fillers", Biomaterials, 7, pp.183~187 (1986)[DOI]
  32. Lee JH, "Fabrication and evaluation of porous beta-tricalcium phosphate", hydroxyapatite(60, ScientificWorld-Journal, pp.2013, 2013[DOI]
  33. Moussa M, "Medium-term function of a 3D printed TCP", HA structure as a new osteoconductive scaffold for vertical bone augmentation: a simulation by BMP-2 activation, 2015, pp.8, 2174~2190
  34. Cory AH, "Use of an aqueous soluble tetrazolium", formazan assay for cell growth assays in culture, 1991, pp.3, 207~212
  35. Le Guehennec L, "Osteoblastic cell behaviour on different titanium implant surfaces", Acta Biomater, 4, pp.535~543 (2008)[DOI]
  36. Kim JH, "The effect of nanotubular titanium surfaces on osteoblast differentiation", J Nanosci Nanotechnol, 10, pp.3581~3585 (2010)[DOI]
  37. Kim JW, "Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coliderived recombinant human bone morphogenetic protein 2 in rat calvarial defects", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 112, pp.298~306 (2011)[DOI]
  38. Strobel LA, "Induction of bone formation in biphasic calcium phosphate scaffolds by bone morphogenetic protein-2 and primary osteoblasts", J Tissue Eng Regen Med, 8, pp.176~185 (2014)[DOI]
  39. Eun-Ung Lee, "Comparative evaluation of biphasic calcium phosphate and biphasic calcium phosphate collagen composite on osteoconductive potency in rabbit calvarial defect", 생체재료학회지, 19(1), pp.50~56 (2015), 한국생체재료학회[DOI]
  40. Shin YS, "The effects of ErhBMP-2-", EGCG-coated BCP bone substitute on dehiscence around dental implants in dogs, 2014, pp.20, 281~287
  41. 손병진, "Ridge preservation using basic fibroblast growth factor-2 and collagenated biphasic calcium phosphate in beagle dogs", Journal of Periodontal & Implant Science, 47(6), pp.381~387 (2017), 대한치주과학회
  42. Cesar R, "Axial compressive strength of human vertebrae trabecular bones classified as normal, osteopenic and osteoporotic by quantitative ultrasonometry of calcaneus", Res Biomed Eng, 33, pp.91~96 (2017)[DOI]
  43. Wang PE, "Sintering behaviour and mechanical properties of hydroxyapatite and dicalcium phosphate", J Mater Sci Mater Med, 4, pp.150~158 (1993)[DOI]
  44. Ryu HS, "An improvement in sintering property of beta-tricalcium phosphate by addition of calcium pyrophosphate", Biomaterials, 23, pp.909~914 (2002)[DOI]
  45. Kellman R, "Atlas of craniomaxillofacial fixation", pp.283~312 (1995), Raven Press

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