비예기 항체 선별 검사의 예민도에 영향을 미치는 인자 연구

Abstract

Unexpected antibody Screening is essential for transfusion practice to prevent hemolytic transfusion reaction. So it is very important to maintain high sensitivity in unexpected antibody screening test. We analyzed the detection limits of anti - D antibody in various test and sample condition to estimate the influence of factors on the sensitivity of unexpected antibody screening test. Regardless of washing procedure, the diluted anti - D antibody with normal saline were detected with same sensitivity by microplate method. In the detection of the diluted anti-D antibody in serum by microplate method, omittion of washing procedure made the sensitivity low. With column agglutination method, the detection sensitivity of the diluted anti-D antibody in serum was higher than that in saline. In conclusion, the neutralization of anti - human globulin have major influence on the sensitivity of antibody detection by microplate method. But the factor having major influence on the sensitivity of column agglutination method is the other one than the neutralization of anti-human globulin.