Rh계 항체 검출을 위한 여러 가지 검사법의 평가

Abstract

Rh system antibodies are frequently implicated in hemolytic disease of the newbom and acute or delayed hemolytic transfusion reaction. To prevent these diseases, irregular antibodies must be screened and identified. Detection of Rh system antibodies is usually done by conventional serological methods. Recently, flow cytomeσy has been introduced in immunohematology to detect antigens and antibodies. We evaluated the possibility of clinical usage to detect Rh system antibodies by flow cytometry. We examined 6 specimens of Rh antibodies (1 anti-C+e, 2 anti-D, 2 anti-E, 1 anti-E+c) that were quantified of Rh antibodies by conventional serological methods (antiglobulin test, PEG test, gel card test, papain treated gel card test) and flow cytometer, and compared the results. The papain treated gel card test showed the highest sensitivity method to detect Rh antibodies. The result of flow cytometer was well correlated with conventional serological methods especially gel card test, except in 3 samples (shown high titers). The results of flow cytometer were objective and reliable for detection and quantification of Rh antibodies. Discrepancies in the results of this study suggest that seem to be influenced by antibodies of high concentration in serum samples.