적혈구 불규칙항체 선별검사의 평가

Abstract

The objective of this study was to detection irregular antibodies among 53 sera by the use of the conventional tube test, low ionic strength solution anti -human globulin(LISS-AHG) test, polyethylene glycol anti-humanglobulin(PEG-AHG) test, gel lowionic strength solution(GEL-LISS) test, and gel papain(Gel-PAP) test. these tests were used to compare their sensitivities and reactivities for the detections. The sera were collected from three hospitals and two blood centers and all were identified to have alloantibodies. Out of 27 clinical1y significant cases, the Gel-PAP test was 100% sensitive in detecting Rh antibodies, the PEG-AHG test was sensitive in detecting Duffy, Kidd, Ss antibodies but not Rh antibodies, and the Gel-LISS test was determined to be sensitive for Rh, Duffy, Kidd, Ss antibodies. The reactivities of these tests for the detection of clinically significant antibodies were well correlated with those of sensitivities. The other 26 sera contained clinically insignificant antibodies(anti-Lea, -Leb, -M, -Pl) and these were detected completely by the tube test. In our experience, Gel-LISS was more sensitive than other antibody screening tests indetecting clinical1v significant antibodies. but we recommend combining methods, for exarnple either the PEG-AHG and Enxyme method together, or the Gel-LISS and the Gel-P AP for more reliable the detection of clinically significant antibodies in sera.