위점막에서 헤마톡실린 염색시간을 연장한 메이어 헤마톡실린-에오신염색에 의한 Helicobacter pylori-like organism 검출률과 특수 염색법들과의 비교

Abstract

Helicobacter pylori-like organism(HLO) is more easily visualized with special stainings than with hematoxylin-eosin, but this is time-consumed, expensive to use the routine diagnostic workup. We performed to verify whether Mayer′s hematoxylin-eosin staining method withprolonged hematoxylin time compares favorably with other well established specia1 stainings in the detection of HLO in gastic biopsy. Fifty gastric biopsy specimens(48 from antrum, two from body) , routinely formaiin fixed and paraffin wax embedded, from gastritis patients were stained with modified Harris′ hematoxylin-eosin(m-Harris HE) and modified Mayer′s hematoxylineosin(m-Mayer HE) with prolonged hematoxylin time( 10min) , Warthin-Starry, Genta, AgNOR and EI-Zimaity staining. The detection rate for HLO was examined by divide into three groups(mild, moderate, severe) according to the degree of inflammation in the biopsy specimens. Density of HLO was scored from 0 to 5 by Genta′s classification. Using Warthin-Starry staining as a standard, positivity for HLO was 52% (26⁄50). Relative sensitivity for Warthin-Starry staining was 100% with m-Mayer HE, 96% with Genta, 96% with EI-Zimaity, 92% with AgNOR, and 80% with m-Harris HE staining. Relative sensitivity of HLO density for Warthin-starry stainings was lower at low (grade 1) density(Genta, 96%; EI-Zimaity. 96%; AgNOR, 33%, routine Harris HE, 0%) than at high(grade 2 to 5) density except case with m-Mayer HE(100%). Detection rate and density of HLO also appeared a significantly high according toincrease the degree of inflammation.The m-Mayer HE staining has a sensitivity comparable to thoes of Warthin-Starry and Genta staining and is simple, inexpensive compared with the other staining methods in detecting HLO. Therefore, it is vety useful and practical for identifying HLO in routine gastric biopsy specimens.