H. pylori 검출을 위한 CLO 검사, Geimsa 염색 및 면역조직화학 염색의 비교 분석

Abstract

In order to detect a rational method for reducing the ratios of the false positive and the false negative appearing from the CLO test and giemsa staining used for the examination of H. pylori, we have compared with the results of CLO test and giemsa staining of which the false positive and the false negative are shown through this study. We have divided the groups as following four groups: A, B, C, and D group. And as for the respective group we have executed the immunohistochemical staining reported as the highest in terms of sensitiveness and particularity and have got to the following results through the reciprocal comparisons of H. pylori detection results. 1. A Group; CLO test positive and giemsa staining positiveAs a result, immunohistochemical staining showed a higher degree rather than giemsa staining in terms of sensitiveness. 2. B Group; CLO test negative and giemsa staining positiveAs a result, false positive of giemsa staining against the results of the negative of immunohistochemical staining was elaborated as stained in the mucus and the similar bacteria. And the false negative of CLO test that does not coincide with the results of immunohistochemical staining was elaborated as inadequacy of the option of the organic extraction. In such a way, immunohistochemical staining showed a higher degree rather than CLO test in terms of the sensitiveness and particularity. 3. C Group; CLO test positive and Giemsa staining negativeAs a result, false negative was judged as stained bacteria as the number of H. pylori is not so many or colored in blue color in the stain background in the giemsa staining. And the false positive of CLO test was elaborated as polluted from bacteria in the inside of the oral cavity at the time of organic extraction. 4. D Group; CLO test negative and giemsa staining negativeImmunohistochemical staining showed negative in all for cases, and coincided with the results of CLO test and giemsa staining. In conclusion, immunohistochemical staining was made in only H. pylori through antigen and antibody. CLO test and giemsa staining showed positive and negative in gastric mucosa. Therefore, as for the detection body of which the results of CLO test and giemsa staining does not coincide like immunohistochemical staining, to let reconfirm through the examination method of high sensitiveness and high characteristics would be of help for an exact detection of H. pylori and an earlier treatment.