진균 예비 동정의 새로운 배지에 관한 연구

Abstract

The authors have tried to make a potato commeal Tween 80 medium containing caffeic acid(PDCC) more convenient for routine mycological laboratory study than Sabouraud dextrose agar. Five species were culture on PDCC at 25ºC and 37ºC. Gross and microscopic fmdings of their colonies were examined. The colonial morphology and microscopic appearance were compared with those of the same strains on caffeic acid agar(CAA). Cryptococcus neoformans Which is a medically important and whose colony showed brown to purple brown on PDCC medium was distinguishable not only from buff colored C. laurentii after one week incubation but also from Candida spp. Candida albicans also formed chlamydospores and pseudohyphae on PDCC. Trichophyton mentagrophytes showed yellow- brown pigmentation which were rapid and darker in the center with gradual fading to the periphery. The macroconidia grape-like clusters of microconidia and spiral hyphae were better on PDCC. The gross morphology of Microsporum canis, Epidermophyton floccosum were similar on PDCC, CAA but macroconidia were seen more typically on PDCC.