객담에서 분리한 Aspergillus 속의 RAPD를 이용한 분자생물학적 동정의 유용성
Availability of Identification by RAPD of Aspergillus species from Sputum
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
.
Abstract
On the basis of morphological characteristics, of total 128 strains of from sputum of tuberculos inpatient were identified as A. fumigatus (61 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characteristics. The strains were grouped by randomly amplified polymorphic DNA (RAPD) techniques. The representative strains from each group were sequenced with partial β-tubulin gene and compared with those of reference strains in the Aspergillus and were identified by the sequence. The identification was confirmed by morphology examination. As the results, they are reidentified as A. fumigatus (58), A. niger (11), A. tubingensis (26), A. flavus (27), A. sydowii (3), A. nidulans (1), A. clavatus (1) and Neosatorya fennelliae (1). This is the first report of A. tubuingensis in clinical field in Korea.
참고문헌 (15)
- Balajee SA, "Aspergillus species identification in the clinical setting", Stud Mycol, 59, pp.39~46 (2007)
- Hong SB, "Polyphasic taxonomy of Aspergillus fumigatus and related species", Mycologia, 97(6), pp.1316~1329 (2005)
- Klich MA, "Identification of common Aspergillus species" (2002), Central bureau voor Schimmel cultures
- Kozakiewicz Z, "Aspergillus species on stored products", Mycological paper, 161, pp.1~25 (1989)
- Kumar S, "MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment", Brief Bioinform, 5, pp.150~163 (2004)
- Peterson SW, "Phylogenetic analysis of Aspergillus species using DNA sequences from four loci", Mycologia, 100, pp.205~226 (2008)
- Samson RA, "Aspergillus", pp.1~18 (1994), Plenum Press
- Samson RA, "Introduction to food and air-borne fungi", pp.64~97 (2004), Central bureau voor Schimmel cultures
- Samson RA, "Diagnostic tools to identify black aspergilli", Stud Mycol, 59, pp.129~145 (2007)
- Varga J, "Aspergillus brasiliensis sp. nov., a biseriate black Aspergillus species with world-wide distribution", Int J Syst Evol Microbiol, 57(Pt 8), pp.1925~1932 (2007)
- 김영권, "임상진균학", pp.44~45 (2008), 고려의학
- 장윤석, "호흡기 아스페르길루스증 -알레르기성 기관지폐 아스페르길루스증을 중심으로", 대한의진균학회지, 7(2), pp.63~68 (2002)
- 정유선, "피부 아스페르길루스증", 대한의진균학회지, 7(1), pp.6~13 (2002)
- 신종희, "임상검체에서 분리된 Aspergillus species에 대한 Random Amplified Polymorphic DNA 분석", 대한임상미생물학회지, 4, pp.33~39 (2001)
- 신종희, "한국인의 임상분리 아스페르길루스 균주에 대한 보리코나졸의 생체외 감수성", 대한의진균학회지, 9(3), pp.166~173 (2004), 대한의진균학회