잠재지문 현출시약들이 인공 지문용액 내 DNA 분자의 STR 분석에 미치는 영향
Influence on STR Analysis of DNA Molecule in Artificial Fingerprint Solution using Latent Fingerprint Reagents
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참고문헌
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초록
본 연구에서는 동일한 조건의 지문을 얻기 위해 아미노산 용액에 지질성분과 DNA를 혼합한 ‘DNA 인공지문 용액’을 개발하고, 10가지 대표적인 잠재지문 현출시약이 STR 분석에 미치는 영향을 확인하였다. DNA 인공지문 용액에 잠재지문 현출시약을 처리한 후 1일, 5일, 10일이 경과함에 따라 추출된 DNA를 분석하였다. 시간 경과에 따라 질산은, 흑색분말, SPR, 수단블랙에서는 일부 대립유전자가 검출되지 않았고, DNA 양이 감소하는 경향을 보였다. 10가지 잠재지문 현출시약 중 닌히드린과 형광분말이 STR 분석에 가장 적게 영향을 미쳤으며, 질산은과 SPR이 STR 분석에 가장 크게 영향을 미쳤다. DNA 인공지문 용액은 동일한 조건의 잠재지문을 제조할 수 있기 때문에, 잠재지문 현출시약이 STR 분석에 미치는 영향에 대한 연구의 재현성과 신뢰성을 높이는데 효과적으로 사용될 수 있을 것으로 판단된다.
Abstract
In this study, we developed ‘DNA artificial fingerprint solution (DAFS)’, which contains lipid components and DNA in amino acid solution in order to obtain fingerprints under the same conditions. Furthermore, the effects of ten representative latent fingerprint reagents using the DAFS on STR analysis were observed. We treated the latent fingerprint reagents in the DAFS, and performed DNA analysis during 1, 5, and 10 days. As time passed, the results showed that some alleles were not detected in silver nitrate, black powder, SPR, and sudan black, and DNA quantity decreased. While ninhydrin and fluorescent powder had the least influence on STR analysis, silver nitrate and SPR had the greatest influence on STR analysis. In conclusion, the fingerprint samples under the same conditions prepared using the DAFS could increase the reproducibility and reliability for the study.
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