DNA가 포함된 법과학 인공혈액의 혈흔형태분석 적용
Application of DNA-containing Forensic Blood Substitute in the Bloodstain Pattern Analysis
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본 저자에 의하여 개발된 인공혈액을 이용하여 혈흔형태 실험의 다양성을 제공하여 활용도를 높이고자 선행연구들의 방법론과 같이 DNA의 염기서열의 조합이나 크기 차이의 다양성을 활용하는 수단으로 합성DNA를 이용하였다. 본 연구에서는 제작된 합성DNA를 개발인공혈액에 적용, PCR을 통해 합성DNA의 크기를 확인함으로써 개발인공혈액을 식별하고자 하였다. 이에 따라 개발인공혈액 내 합성DNA 를 증폭하기 위한 조건을 확립하고, 합성DNA의 안정성 시험을 통한 검증을 실시하였으며, 설계 및 제작한 합성DNA를 개발인공혈액에 적용함으로써 기존의 물리적 특성을 부각한 개발인공혈액에 다양성을 부여할 수 있었다. 혈흔형태분석 훈련에서 다수인의 혈흔이 혼재된 상황을 가정하였을 때 DNA 정량을 통한 혈액의 비율 추정이 가능하며, 이를 통한 혈흔형태분석 적용이 가능함을 보여주었다. 이를 통해 실제인체혈액에서 DNA분석을 통해 개인식별이 가능한 것과 유사하게 식별이 가능한 개발인공혈액으로서의활용도를 높일 수 있었다
Abstract
We used a developed blood substitute and synthetic DNA to test the combination of different DNA sequences or sizes to increase the availability of the BPA. In this study, we produced synthetic DNA, applied to blood substitute, identified the size through PCR, thereby identified characteristics. Accordingly, we established conditions for amplifying synthetic DNA in blood substitute and verified the stability of synthetic DNA. We can diversify blood substitutes that emphasized the existing physical characteristics by adding the synthetic DNA. Also, we suggested that BPA will be available by estimating the proportion of blood through quantification of DNA in the mixed blood, assuming that bloodstains from multiple people are mixed in training. By doing so, we could improve the availability of developed blood substitute to the point where it can be identified similarly as in DNA analysis from human blood.
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