Cyanoacrylate를 이용한 잠재지문 현출 이후 유전자 감정을 위한 채취 용액의 선정
Selection Condition of Swabbing Solution for Genetic Analysis after Latent Fingerprint Identification using Cyanoacrylate Fuming
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
본 연구에서는 CA 훈증하여 현출된 지문으로부터 유전자를 효과적으로 채취하기 위하여 순간접착제를 용이하게 제거할 수 있는 용매를 채취용액으로 사용하였으며 DNA 회수율 및 STR 분석 성공률을 비교 분석하여 새로운 채취 방법으로서의 활용 가능성을 검토하고자 하였다. 실제 지문과 땀 유사용액 및 유전자를 혼합한 시료를 CA 훈증하고, 선정된 채취용액으로 DNA 검출과 STR 분석을 시행하였다. 현재 적용하는 증류수나 생리식염수와 더불어 본 연구에서 사용된 채취용액들도 CA 훈증 이후 연속검출을 위한 지문 잔류 DNA 회수에 용이한 채취용매로 사용이 가능할 것으로 판단되었다. 그리고 적용한 채취용액 중, 가장 좋은 채취용액으로 선정된 용매는 acetonitrile과 dichloromethane 이었으며 극미량의 DNA도 성공적으로 회수 가능하였고 STR분석 결과도 우수한 결과를 보여주었다.
Abstract
In this study, main compositions of commercialized cyanoacrylate adhesive removers were evaluated as swabbing solutions to recover trace DNA samples from CA fumed fingerprints. The efficiency of DNA recovery were determined by comparing DNA concentration and STR success rate. After fingerprints and gene samples in sweat enhanced by CA fuming method, samples were collected with swabbing solutions and DNA detection and STR analysis were performed. The results indicate that organic solvents could be used as swabbing solution for genetic analysis after latent fingerprint identification using CA fuming, along with distilled water and saline solution which are widely available and routinely used in sampling. Acetonitrile and dichloromethane showed the best performance in trace DNA recovery and STR analysis.
참고문헌 (23)
- 정지혜, "잠재지문 현출시약이 인공 지문 용액 내 DNA 분석에 미치는 영향", 석사
- 조서린, "수제 접촉각 측정기와 영상 분석 프로그램을 이용한 표면에너지의 측정", 대한화학회지, 57(4), pp.432~438 (2013), 대한화학회[DOI]
- Al‐Ghamdi, S. S., "Toluene and p-xylene induced LLCPK1 apoptosis", Drug and chemical toxicology, 27(4), pp.425~432 (2004)
- Andersen, M. B, "Unconsciousness after ingestion of nail varnish", Ugeskrift for laeger, 154(44), pp.3064~ (1992)
- Dadmun, M. D., "Cultivating methods to enhance the quality of aged fingerprints developed by cyanoacrylate fuming", Bibliogov USA, ~ (2009)
- Duvvi, S.K, "Superglue (cyanoacrylate) in the nose", Otolaryngology-Head and Neck Surgery, 133(5), pp.803~804 (2005)
- Edwards, H. G, "Anomalies in polycyanoacrylate formation studied by Raman spectroscopy : Implications for the forensic enhancement of latent fingerprints for spectral analysis", Vibrational spectroscopy, 41(2), pp.155~159 (2006)
- Higgins Jr, T. F, "Coma and respiratory arrest after exposure to butyrolactone", The Journal of emergency medicine, 14(4), pp.435~437 (1996)
- Moschos, M., "Clinical experience with cyanoacrylate tissue adhesive", Documenta ophthalmologica, 93(3), pp.237~245 (1997)
- Pang, B. C. M, "Double swab technique for collecting touched evidence", Legal Medicine, 9(4), pp.181~184 (2007)
- Pariselli, F., "Effects of toluene and benzene air mixtures on human lung cells(A549)", Experimental and Toxicologic Pathology, 61(4), pp.381~386 (2009)
- Picton-Robinson, I., "Danger of instant adhesives", British medical journal, 2(6086), pp.581~ (1977)
- Shantha, K. L., "Developments and applications of cyanoacrylate adhesives", Journal of adhesion science and technology, 3(1), pp.237~260 (1989)
- Sinelnikov, A, "Materials and methods that allow fingerprint analysis and DNA profiling from the same latent evidence", Forensic Science International: Genetics Supplement Series, 6, pp.e40~e42 (2017)
- Singh, M. P, "Genotoxicity and apoptosis in Drosophila melanogaster exposed to benzene, toluene and xylene: Attenuation by quercetin and curcumin", Toxicology and applied pharmacology, 253(1), pp.14~30 (2011)
- Thamnurak, C., "DNA typing from fluorescent powder dusted latent fingerprints", Forensic Science International: Genetics Supplement Series, 3(1), pp.e524~e525 (2011)
- Tikka, T., "Deliberate self-harming application of superglue in the nose:case report and literature review", The Journal of laryngology &otology,, 129(1), pp.98~ (2015)
- Van Oorschot, RA., "Forensic trace DNA: a review", Investigative Genetics, 1(1), pp.14~ (2010)
- Velthuis, S., "Studies toward the development of a positive control test for the cyanoacrylate fuming technique using artificial sweat", Journal of Forensic Identification, 61(1), pp.16~ (2011)
- Wickenheiser, RA., "Trace DNA: a review, discussion of theory and application of the transfer of trace quantities of DNA through skin contact", Journal of Forensic Science, 47(3), pp.442~450 (2002)
- "엑시아 R2, 자이안 순간접착제 제거제, Good Off Super Glue Remover, Everbuild Stick 2 Superglue Remover, Pacer Technology Super Glue Remover, Damn Good™ SuperGlue Cleaner", ~
- "전자부품용 순간접착제 리무버 조성물 등록특허 10-0645633 등록일자 2006년11월6일", Composition for removing cyanoacrylate adhesives from surfaces Appl. No. 293,872 April 26, 1983, ~ (1983)
- Gamma-butyrolactone(GBL) Pre-Review Report, "Expert Committee on Drug Dependence Thirty-fifth Meeting", WHO, ~ (2012)