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비만세포에서 連翹 추출물의 Th2 사이토카인 발현 및 신호전달 기전 억제효과

The Suppressive Effect of Th2 Cytokines Expression and the Signal Transduction Mechanism in MC/9 Mast Cells by Forsythiae Fructus Extracts

김윤희|한재경|이진화
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2014.08|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제28권 제3호|pp. 31-46|DOI http://dx.doi.org/10.7778/jpkm.2014.28.3.031

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키워드

Mast cellForsythiae FructusTh2 cytokinesDNP-IgE
주제분류한의학

초록

ObjectivesForsythiae Fructus treatment has been used for inflammatory and allergic diseases in Korean Medicine. Nevertheless, the mechanism of action and the cellular targets are not understood well. The pathogenesis of allergicdiseases are associated with Th2 cytokines such as IL-13, MIP-1α, IL-13, IL-5, GM-CSF, IL-4, TNF-α and IL-6,which are secreted by the mast cells. This study was conducted to investigate the effects of Forsythiae Fructusextracts (FF) on Th2 cytokines expression and signal transduction in MC/9 mast cells. MethodsIn the study, MC/9 mast cells were stimulated with DNP-IgE for 24 hours and then treated separately with CsA10 ㎍/㎖ and varying doses of FF for one hour. MC/9 mast cells stimulated with DNP-IgE was the control group,a treatment with CsA was the positive control group and a treatment with varying doses FF was the experimentalgroups. The mRNA levels of IL-13, IL-5, GM-CSF, IL-4, TNF-α, IL-6 were analyzed with Real-time PCR. Thelevels of IL-13, MIP-1α were measured using enzyme-linked immunosorbent assays(ELISA). NFAT, AP-1 and NF-κB p65 were examined by Western blot analysis. Results1. FF were observed to suppress the mRNA expression of IL-13, IL-5, GM-CSF, IL-4, TNF-α, IL-6 in comparisonto DNP-IgE control group. 2. FF also has inhibited the IL-13, MIP-1α production significantly in comparison to DNP-IgE control group. 3. Western blot analysis of transduction factors involving Th2 cytokines expression has revealed a prominentdecrease of the mast cell specific transduction factors including NFAT-1, NFAT-2, c-Jun, and NF-κB p65 butc-Fos. ConclusionsIn conclusion, the anti-allergenic activities of FF may be strongly related to the regulation of transcription factorsNFAT-1, NFAT-2, c-Jun, and NF-κB p65 causing inhibition of Th2 cytokines in mast cells.

Abstract

ObjectivesForsythiae Fructus treatment has been used for inflammatory and allergic diseases in Korean Medicine. Nevertheless, the mechanism of action and the cellular targets are not understood well. The pathogenesis of allergicdiseases are associated with Th2 cytokines such as IL-13, MIP-1α, IL-13, IL-5, GM-CSF, IL-4, TNF-α and IL-6,which are secreted by the mast cells. This study was conducted to investigate the effects of Forsythiae Fructusextracts (FF) on Th2 cytokines expression and signal transduction in MC/9 mast cells. MethodsIn the study, MC/9 mast cells were stimulated with DNP-IgE for 24 hours and then treated separately with CsA10 ㎍/㎖ and varying doses of FF for one hour. MC/9 mast cells stimulated with DNP-IgE was the control group,a treatment with CsA was the positive control group and a treatment with varying doses FF was the experimentalgroups. The mRNA levels of IL-13, IL-5, GM-CSF, IL-4, TNF-α, IL-6 were analyzed with Real-time PCR. Thelevels of IL-13, MIP-1α were measured using enzyme-linked immunosorbent assays(ELISA). NFAT, AP-1 and NF-κB p65 were examined by Western blot analysis. Results1. FF were observed to suppress the mRNA expression of IL-13, IL-5, GM-CSF, IL-4, TNF-α, IL-6 in comparisonto DNP-IgE control group. 2. FF also has inhibited the IL-13, MIP-1α production significantly in comparison to DNP-IgE control group. 3. Western blot analysis of transduction factors involving Th2 cytokines expression has revealed a prominentdecrease of the mast cell specific transduction factors including NFAT-1, NFAT-2, c-Jun, and NF-κB p65 butc-Fos. ConclusionsIn conclusion, the anti-allergenic activities of FF may be strongly related to the regulation of transcription factorsNFAT-1, NFAT-2, c-Jun, and NF-κB p65 causing inhibition of Th2 cytokines in mast cells

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