교맥의 RBL-2H3 비만세포 탈과립과 cytokine 생산 억제 효과
Inhibitory effect of Fagopyrum esculentum on degranulation and production of cytokine in RBL-2H3 cells
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Objectives : Fagopyrum esculentum(FE) has been used for removal of inflammation of internal organs and treatment of sore and ulcer by heat toxin in Korean herbal medicines. In this study, To investigated the protective effect of FE on allergic response, we determined whether FE inhibits allergic response. Methods : The effect of FE was analyzed by ELISA, RT-PCR and Western blot in RBL-2H3 cells. We investigated cell viability, β-hexosaminidase, as a marker of degranulation, cytokne, and intracellular ROS and MAPK and NF-κB signaling. Results : We found that FE suppressed β-hexosaminidase release, the production of IL-4 and TNF-α and intracellular ROS level in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. FE also significantly inhibited cytokine mRNA expressions, such as IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-13, TNF-α and GM-CSF in RBL-2H3. In addition, PF suppressed the phospholyation of ERK1/2, JNK1/2, p38 and IκBα and NF-κB signal transduction pathway. Conclusions : Our results indicate that FE protects against allergic response and exerts an anti-inflammatory effect through the inhibition of degranulation and production of cytokines and ROS via the suppression MAPK and NF-κB of signal transduction. Abbrevations : FE, Fagopyrum esculentum; RBL-2H3, rat basophilic leukemia cell line; ROS, reactive oxygen species; MAPK, Mitogen-activated protein kinase; NFκB, nuclear factor κB; TNFα, Tumor necrosis factor alpha;GM-CSF, Granulocyte macrophage colony-stimulating factor; ERK, extracellular-signal-regulated kinase; JNK, c-Jun NH2-terminal kinase; p38, p38 MAP kinase; IκBα, inhibitory-kappa B alpha
Abstract
Objectives : Fagopyrum esculentum(FE) has been used for removal of inflammation of internal organs and treatment of sore and ulcer by heat toxin in Korean herbal medicines. In this study, To investigated the protective effect of FE on allergic response, we determined whether FE inhibits allergic response. Methods : The effect of FE was analyzed by ELISA, RT-PCR and Western blot in RBL-2H3 cells. We investigated cell viability, β-hexosaminidase, as a marker of degranulation, cytokne, and intracellular ROS and MAPK and NF-κB signaling. Results : We found that FE suppressed β-hexosaminidase release, the production of IL-4 and TNF-α and intracellular ROS level in RBL-2H3 by the anti-DNP IgE plus DNP-HSA stimulation. FE also significantly inhibited cytokine mRNA expressions, such as IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-13, TNF-α and GM-CSF in RBL-2H3. In addition, PF suppressed the phospholyation of ERK1/2, JNK1/2, p38 and IκBα and NF-κB signal transduction pathway. Conclusions : Our results indicate that FE protects against allergic response and exerts an anti-inflammatory effect through the inhibition of degranulation and production of cytokines and ROS via the suppression MAPK and NF-κB of signal transduction. Abbrevations : FE, Fagopyrum esculentum; RBL-2H3, rat basophilic leukemia cell line; ROS, reactive oxygen species; MAPK, Mitogen-activated protein kinase; NFκB, nuclear factor κB; TNFα, Tumor necrosis factor alpha;GM-CSF, Granulocyte macrophage colony-stimulating factor; ERK, extracellular-signal-regulated kinase; JNK, c-Jun NH2-terminal kinase; p38, p38 MAP kinase; IκBα, inhibitory-kappa B alpha
참고문헌 (29)
- Beasley R, "International patterns of the prevalence of pediatric asthma", The ISAAC program. Pediatr. Clin. North Am, 50, pp.539~553 (2003)
- Jaakkola JJK, "Asthma, Wheezing, and Allergies in Russian Schoolchildren in Relation to New Surface Materials in the Home", Am J Public Health, 94(4), pp.560~562 (2004)
- Amin K, "The role of mast cells in allergic inflammation", Respiratory Medicine, 106(1), pp.9~14 (2012)
- Brooks AC, "Reactive oxygen species generation and histamine release by activated mast cells : modulation by nitric oxide synthase inhibition", Br J Pharmacol, 128(3), pp.585~590 (1999)
- Matsui T, "Diphenyleneiodonium Prevents Reactive Oxygen Species Generation, Tyrosine Phosphorylation, and Histamine Release in RBL-2H3 Mast Cells", Biochemical and Biophysical Research Communications, 276(2), pp.742~748 (2000)
- Gilfillan AM, "Integrated signalling pathways for mast-cell activation", Nature Reviews Immunology, 6(3), pp.218~230 (2006)
- Klemm S, "Inflammatory signal transduction from the FcεRI to NF-κB", Immunobiology, 211(10), pp.815~820 (2006)
- Hofmann AM, "New Roles for Mast Cells in Modulating Allergic Reactions and Immunity Against Pathogens", Curr Opin Immunol, 21(6), pp.679~686 (2009)
- Li SQ, "Advances in the development of functional foods from buckwheat", Crit Rev Food Sci Nutr, 41(6), pp.451~464 (2001)
- Heo J, "Dongeuibogam. 1st ed", pp.1808~1719 (2007), Beobin munhwasa
- Jugyakdaesajeon pyeonchanwiwonhoe, "Jugyakdaesajeon", pp.392~393 (2006), Jeongdam
- Matsuda H, "Anti-allergic principles from Thai zedoary : structural requirements of curcuminoids for inhibition of degranulation and effect on the release of TNF-α and IL-4 in RBL-2H3 cells", Bioorganic & Medicinal Chemistry, 12(22), pp.5891~5898 (2004)
- Nakano N, "Effects of arachidonic acid analogs on FcepsilonRImediated activation of mast cells", Biochim. Biophys. Acta, 1738(1-3), pp.19~28 (2005)
- Marquardt DL, "Dependence of mast cell IgE-mediated cytokine production on nuclear factor-κB activity", Journal of Allergy and Clinical Immunology, 105(3), pp.500~505 (2000)
- Granberg M, "Effects of the cannabimimetic fatty acid derivatives 2-arachidonoylglycerol, anandamide, palmitoylethanolamide and methanandamide upon IgE-dependent antigen-induced ß-hexosaminidase, serotonin and TNFa release from rat RBL-2H3 basophilic leukaemia cells", Naunyn-Schmiedeberg’s Archives of Pharmacology, 364(1), pp.66~73 (2001)
- Bischoff SC, "Role of mast cells in allergic and non-allergic immune responses : comparison of human and murine data", Nature Reviews Immunology, 7(2), pp.93~104 (2007)
- Metcalfe DD, "Mast cells", Physiol Rev, 77(4), pp.1033~1079 (1997)
- Hines C, "The diverse effects of mast cell mediators", Clinical Reviews in Allergy and Immunology, 22(2), pp.149~160 (2002)
- Grünig G, "Requirement for IL-13 Independently of IL-4 in Experimental Asthma", Science, 282(5397), pp.2261~2263 (1998)
- Gordon JR, "Mast cells as a source of both preformed and immunologically inducible TNF-α", cachectin, 1990, pp.346(6281), 274~276
- Suzuki Y, "FcεRI Signaling of Mast Cells Activates Intracellular Production of Hydrogen Peroxide : Role in the Regulation of Calcium Signals", J Immunol, 171(11), pp.6119~6127 (2003)
- Swindle EJ, "Rodent and Human Mast Cells Produce Functionally Significant Intracellular Reactive Oxygen Species but Not Nitric Oxide", J. Biol. Chem, 279(47), pp.48751~48759 (2004)
- Hur SJ, "Effect of buckwheat extract on the antioxidant activity of lipid in mouse brain and its structural change during in vitro human digestion", J. Agric. Food Chem, 59(19), pp.10699~10704 (2011)
- Zhang C, "Mitogen-activated Protein(MAP) Kinase Regulates Production of Tumor Necrosis Factor-α and Release of Arachidonic Acid in Mast Cells Indication of Communication between p38 and p42 Map Kinases", J. Biol. Chem
- Hirasawa N, "Involvement of a phosphatidylinositol 3-kinasep38 mitogen activated protein kinase pathway in antigen-induced IL-4 production in mast cells", Biochimica et Biophysica Acta(BBA)-Bioenergetics, 1456(1), pp.45~55 (2000)
- Li Q, "NF-κB regulation in the immune system", Nature Reviews Immunology, 2(10), pp.725~734 (2002)
- Azzolina A, "Substance P induces TNF-α and IL-6 production through NFκB in peritoneal mast cells", Biochimica et Biophysica Acta(BBA) - Molecular Cell Research, 1643(1-3), pp.75~83 (2003)
- Karin M., "NF-κB and cancer: Mechanisms and targets", Molecular Carcinogenesis, 45(6), pp.355~361 (2006)
- Karin M, "NF-κB : linking inflammation and immunity to cancer development and progression", Nature Reviews Immunology, 5(10), pp.749~759 (2005)