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MC/9 비만세포에서 杏仁 추출물의 Th2 cytokine 발현 억제 효과 및 신호전달 기전 연구

The Suppressive Effect on Th2 Cytokines Expression and the Signal Transduction Mechanism in MC/9 Mast Cells by PRAL

김윤희|한재경|강기연
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2014.05|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제28권 제2호|pp. 23-39

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참고문헌

키워드

Th2 cytokinesPRAL (Prunus armniaca Linne Var 杏仁)MC/9 mast cellAtopic diseaseAnti allergic effect
주제분류한의학

초록

ObjectivesPRAL (Prunus armniaca Linne Var) is a herbal formula in Oriental Medicine, known for its anti-inflammatoryand anti-allergenic properties. However, its mechanism of action and the cellular targets have not yet been found enough. The purpose of this study is to investigate the effects of PRAL on Th2 cytokines expression in MC/9 mast cells. MethodsThe effect of PRAL was analyzed by ELISA, Real-time PCR, Western blot in MC/9 mast cells. mRNA levels ofGM-CSF, IL-4, IL-5, IL-6, IL-13, TNF-α were analyzed with Real-time PCR. Levels of IL-13, MIP-1α were measuredusing enzyme-linked immunosorbent assays (ELISA). NFAT, AP-1 and NF-κB p65 were examined by Western blot analysis. ResultsPRAL inhibited GM-CSF, IL-4, IL-5, IL-6, IL-13, TNF-α mRNA expression in a dose dependent manner. GM-CSF,IL-4, IL-5 mRNA expression were inhibited significantly in comparison to DNP-IgE control group at concentrationof 100 ㎍/㎖ and IL-6, IL-13, TNF-α mRNA expression were inhibited at concentration of 50 ㎍/㎖, 100 ㎍/㎖. PRAL also inhibited the IL-13, MIP-1α production significantly in comparison to DNP-IgE control group in adose dependent manner. IL-13 production was inhibited at a concentration of 200 ㎍/㎖, 400 ㎍/㎖ and MIP-1αwas inhibited at a concentration of 100 ㎍/㎖, 200 ㎍/㎖, 400 ㎍/㎖. Western blot analysis of transcription factors involving Th2 cytokines expression revealed prominent decrease of themast cell specific transcription factors including NFAT-1, c-Jun as well as NF-κB p65 but not NFAT-2 and c-Fos. ConclusionThese results indicate that PRAL has the effect of suppressing Th2 cytokines production in the MC/9 mast cells. These data represent that PRAL potentiates therapeutic activities to the allergic disease by regulating Th2 cytokinesin the MC/9 mast cells.

Abstract

ObjectivesPRAL (Prunus armniaca Linne Var) is a herbal formula in Oriental Medicine, known for its anti-inflammatoryand anti-allergenic properties. However, its mechanism of action and the cellular targets have not yet been found enough. The purpose of this study is to investigate the effects of PRAL on Th2 cytokines expression in MC/9 mast cells. MethodsThe effect of PRAL was analyzed by ELISA, Real-time PCR, Western blot in MC/9 mast cells. mRNA levels ofGM-CSF, IL-4, IL-5, IL-6, IL-13, TNF-α were analyzed with Real-time PCR. Levels of IL-13, MIP-1α were measuredusing enzyme-linked immunosorbent assays (ELISA). NFAT, AP-1 and NF-κB p65 were examined by Western blot analysis. ResultsPRAL inhibited GM-CSF, IL-4, IL-5, IL-6, IL-13, TNF-α mRNA expression in a dose dependent manner. GM-CSF,IL-4, IL-5 mRNA expression were inhibited significantly in comparison to DNP-IgE control group at concentrationof 100 ㎍/㎖ and IL-6, IL-13, TNF-α mRNA expression were inhibited at concentration of 50 ㎍/㎖, 100 ㎍/㎖. PRAL also inhibited the IL-13, MIP-1α production significantly in comparison to DNP-IgE control group in adose dependent manner. IL-13 production was inhibited at a concentration of 200 ㎍/㎖, 400 ㎍/㎖ and MIP-1αwas inhibited at a concentration of 100 ㎍/㎖, 200 ㎍/㎖, 400 ㎍/㎖. Western blot analysis of transcription factors involving Th2 cytokines expression revealed prominent decrease of themast cell specific transcription factors including NFAT-1, c-Jun as well as NF-κB p65 but not NFAT-2 and c-Fos. ConclusionThese results indicate that PRAL has the effect of suppressing Th2 cytokines production in the MC/9 mast cells. These data represent that PRAL potentiates therapeutic activities to the allergic disease by regulating Th2 cytokinesin the MC/9 mast cells.

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