한방 입욕제 조성물이 MC/9 mast cell에서의 Th2 cytokine 발현에 미치는 영향
Effects of Korean Herbal Bathing Extracts Composition on Th2 Cytokine Production in MC/9 Mast Cells
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives The purpose of this study is to investigate the effects of korean herbal bathing extracts composition 1 and composition 2 on Th2 cytokine production in MC/9 mast cells. Methods The effects of composition 1, 2 was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. Results Composition 1, 2 inhibited the IL-5, IL-13 production signifcantly(p<.001) in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Composition 1, 2 inhibited the IL-4, IL-5, IL-13 mRNA expression signifcantly in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Composition 1 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 200 ㎍/㎖. Composition 2 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Conclusions These results indicate that composition 1, 2 has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.
Abstract
Objectives The purpose of this study is to investigate the effects of korean herbal bathing extracts composition 1 and composition 2 on Th2 cytokine production in MC/9 mast cells. Methods The effects of composition 1, 2 was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. Results Composition 1, 2 inhibited the IL-5, IL-13 production signifcantly(p<.001) in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Composition 1, 2 inhibited the IL-4, IL-5, IL-13 mRNA expression signifcantly in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Composition 1 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 200 ㎍/㎖. Composition 2 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ㎍/mL, 200 ㎍/mL. Conclusions These results indicate that composition 1, 2 has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.
참고문헌 (25)
- Committee of publishing textbook Dermatology, "Dermatology 5th Ed", pp.170~171 (2008), Ryo Moon Gak
- Park YL, "Pathogenesis of Atopic Dermatitis", Korean Journal of Investigative Dermatology, 14(3), pp.67~72 (2007)
- Sandstrom MH, "Prognosis and prognostic factors in adult patient with atopic dermatitis : a long-term follow-up qeustionnaire study", Br J Dermatol, 150, pp.103~110 (2004)
- Robert Sidbury MD, "Old, New, And Emerging Therapies for Atopic Dermatitis", Dermatologic Clinics, 18(1), pp.1~11 (2000)
- Schreiber SL, "The mechanism of action of cyclosporin A and FK506", Immunol Today, 13(4), pp.136~142 (1992)
- Oh PI, "A study on the development of a tub system used by Wiwanua latifolia", 석사, pp.2007
- 한승헌, "한방 입욕제 처방이 피부 병변 모델에 미치는 영향", 한국한의학연구원논문집, 16(3), pp.107~113 (2010), 한국한의학연구원
- 이정필, "한방입욕제가 운동선수의 피로회복 및 생리학적 변인에 미치는 효과", 한국 스포츠 리서치, 16(5), pp.831~840 (2005), 한국스포츠리서치
- Lee BK, "Effective methods of fatigue recovery on the active old aged man", Sports Science, 6(1), pp.73~84 (1997)
- 김수화, "피부의 노화와 주름 억제, 미백효과를 가지는 전통 한약재의 탐색", 동의생리병리학회지, 22(3), pp.691~698 (2008), 대한동의병리학회
- National federation of department of sasang constitutional medicine, colleges of oriental medicine, "Sasang constitutional medicine. 2nd Ed", pp.289~291 (2004), Jipmoondang
- Gu BH, "Chinese medical traumatology in clinical practice", pp.280~282 (1985), Shanghaikexuejishuchubanshe
- Committee of publishing textbook Korean Medicine, "Herbalogy", pp.177~717 (2005), Younglim
- Heo J, "New translation of Donguibogam", pp.1949~2002 (2007), Bubin publishing
- Munetaka Ishiyama, "A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability", Talanta, 44(7), pp.1299~1305 (1997)
- Akdis A, "Immune regulation in atopic dermatitis", Curr Opin Immunol, 12, pp.641~646 (2002)
- Wollenberg A, "Atopic dermatitis : pathogenetic mechanism", Clin Exp Dermatol, 25, pp.530~534 (2000)
- Won JS, "New Compliation of Four constitutional Medicine. 1st", pp.114~ (2002), Jungdam
- Leung DYM, "Pathogenesis of atopic dermatitis", J Allergy Clin Immunol, 104, pp.99~108 (1999)
- Aversa G, Punnonen, "An IL-4 mutant protein inhibits both IL-4 or IL-3-induced human IgG4 and IgE synthesis and B cell proliferation : support for common component shared by IL-4 and IL-13 receptors", J Exp Med, 178, pp.2213~2218 (1993)
- Toshiaki Kawakami, "Regulation of mast-cell and basophil function and survival by IgE", Nature Reviews Immunology, 2, pp.773~786 (2002)
- Taketomi Y, "Lipid networks in mast cell biology", Yakugaku Zasshi, 131(1), pp.73~84 (2001)
- 최희정, "목욕행위와 건강상태와의 관계: 도시지역에 거주하는 여성을 대상으로", Journal of Korean Academy of Nursing, 32(4), pp.519~528 (2002), 한국간호과학회
- 김정수, "알레르기성 접촉 피부염 및 외과적 창상 모델에서 한방 입욕제 원료 처방의 효과", 동의생리병리학회지, 24(3), pp.484~489 (2010), 대한동의병리학회
- 정보경, "한방 기능성 입욕제 SP1과 SP2의 항산화, 항노화, 미백효과에 관한 연구", 동의생리병리학회지, 24(5), pp.837~842 (2010), 대한동의병리학회