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쥐 비만세포 (RBL-2H3)와 OVA/alum 감작 마우스에서 치자 추출물의 알레르기 염증 억제 효과

The Effects of Gardenia jasminoides J.Ellis on Allergic Inflammation in RBL-2H3 Mast Cells and OVA/alum-sensitized Mice

김은진|김윤희|이진화|김경리
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2018.11|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제32권 제4호|pp. 71-86|DOI https://doi.org/10.7778/jpkm.2018.32.4.071

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키워드

Th2 cytokinesRBL-2H3 mast cellOVA/alum-sensitized miceBalb/c miceAllergic inflammation
주제분류한의학

초록

Objectives The purpose of this study is to investigate the effects of Gardenia jasminoides for. grandiflora extracts’ (GAJ) anti-inflammatory effect on RBL-2H3 mast cells and OVA/alum-sensitized mice. Methods In this study, IL-4 and IL-13 production was measured via ELISA analysis, and mRNA expressions of GM-CSF, IL-4, IL-5, TNF-α, IL-6 were analyzed by real-time PCR. In addition, MAPKs and NF-κB p65 transcription factors were examined using western blotting, and ELISA was used to understand IgE, IL-4, and IL-13 production in ovalbumin-allergic mice in in vitro study. Results As a result of this study, 1. GAJ were observed to suppress the mRNA expression of GM-CSF, IL-4, IL-13, IL-5, TNF-α, IL-6 in comparison to PMA 50 ng/㎖, ionomycin 0.5 μM (PI) control group. 2. GAJ also inhibited the IL-4, IL-13 production in comparison to PI control group. 3. Western blot analysis showed decrease on the expression of mast-cell-specific transcription factors, including MAKPs (ERK, JNK, p38) and NF-κB p65. 4. Orally-administered GAJ group in OVA/alum induced Balb/c mice showed decreased level of OVA-specific IgE in the serum. This group also has shown decreased the level of IL-4, IL-13 in the splenocyte culture supernatant. Conclusions Obtained results suggest that GAJ may regulate the allergic inflammation by transcription factors MAKPs (ERK, JNK, p38) and NF-κB p65 causing inhibition of Th2 cytokines in mast cells and OVA/alum-sensitized mice.

Abstract

Objectives The purpose of this study is to investigate the effects of Gardenia jasminoides for. grandiflora extracts’ (GAJ) anti-inflammatory effect on RBL-2H3 mast cells and OVA/alum-sensitized mice. Methods In this study, IL-4 and IL-13 production was measured via ELISA analysis, and mRNA expressions of GM-CSF, IL-4, IL-5, TNF-α, IL-6 were analyzed by real-time PCR. In addition, MAPKs and NF-κB p65 transcription factors were examined using western blotting, and ELISA was used to understand IgE, IL-4, and IL-13 production in ovalbumin-allergic mice in in vitro study. Results As a result of this study, 1. GAJ were observed to suppress the mRNA expression of GM-CSF, IL-4, IL-13, IL-5, TNF-α, IL-6 in comparison to PMA 50 ng/㎖, ionomycin 0.5 μM (PI) control group. 2. GAJ also inhibited the IL-4, IL-13 production in comparison to PI control group. 3. Western blot analysis showed decrease on the expression of mast-cell-specific transcription factors, including MAKPs (ERK, JNK, p38) and NF-κB p65. 4. Orally-administered GAJ group in OVA/alum induced Balb/c mice showed decreased level of OVA-specific IgE in the serum. This group also has shown decreased the level of IL-4, IL-13 in the splenocyte culture supernatant. Conclusions Obtained results suggest that GAJ may regulate the allergic inflammation by transcription factors MAKPs (ERK, JNK, p38) and NF-κB p65 causing inhibition of Th2 cytokines in mast cells and OVA/alum-sensitized mice.

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