Inhibitory Effects of Jojoongikgi-tang on the secretion of PGE2 and NO and Cytokines in LPS-stimulated BV2 microglial Cells
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Abstract
Inhibitory Effects of Jojoongikgi-tang on the Secretion of PGE2 and NO and Cytokines in LPS-stimulated BV2 Microglial Cells Lee Jun Moon, Lyu Sun Ae, Lee Seung Yeon*, Kim Bo Kyung**, Ko Woo Shin*** Department of Pediatrics, College of Oriental Medicine, Dong-Eui University *Department of Pediatrics, College of Oriental Medicine, Dong-Eui University **Clinical Research Center of Oriental Medicine, Dong-Eui University Department of Neuropsychiatry, College of Oriental Medicine, Dong-Eui University ***Department of Oriental Medical Ophthalmology & Otolaryngology &Dermatology, College of Oriental Medicine, Dong-Eui University Objectives This experiment is about Jojoongikgi-tang(JIT) exerts anti-inflammatory effects in BV2 microglial cells, and the effect of JIT on Nitric oxide(NO) production in lipopolysaccharide(LPS)-stimulated BV2 microglial cells were also demonstrated. Methods To investigate the anti-inflammatory effects of JIT, NO production, expression level of iNOS mRNA, PGE2 synthesis, expression COX-2 mRNA, cell viability, TNF-α mRNA expression were examined. Results The expression level of inducible nitric oxide synthase(iNOS) was decreased by JIT, and the production of Prostaglandin E2(PGE2) and the expression of Cox-2 mRNA also were inhibited by JIT. Proinflammatory mediators, such as TNF-α, IL-1β, IL-12, were inhibited by JIT in a dose-dependent manner. Conclusions JIT have anti-inflammatory effects in BV2 microglial cells and could be used in inflammatory disease.
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