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Inhibitory Effects of Hwanggigunjung-tang on the secretion of PGE2, NO and Cytokines in LPS-stimulated BV2 microglial Cells

이승연|홍상훈|유선애|고우신|윤화정|신상호|류지효|임영우
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2007.08|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제20권 제2호|pp. 116-131

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초록

黃芪健中湯은 補裨益氣 및 祛濕熱하는 效能으로 身體의 虛弱을 개선하며 炎症을 유발할 수 있는 外邪의 침입을 방어하여 인체의 免疫機能을 강화하는 처방으로 면역관련 세포중 하나인 microglial cell를 대상으로 면역세포에 黃芪健中湯이 어떠한 효능을 발휘하는 지를 살펴보았다. 물로 煎湯한 黃芪健中湯을 여과한 후 실험한 결과 BV2 microglial cells에서 LPS로 자극하여 염증을 유발하는 주요 물질인 NO의 분비를 세포독성 없이 억제하였다. 또한NO를 생성하는 효소인 iNOS의 합성도 감소되었다. 그리고 염증관련 인자중 하나인 PGE2의 생성도 억제되었고 PGE2를 생성하는 효소인 Cox-2의 발현도 감소시켰다. 그리고 기타 염증전구 물질들인 TNF-α, IL-1β, IL-12도 黃芪健中湯의 용량이 증가함에 따라 의존적으로 감소함을 알 수 있었다. 이러한 결과를 보아 황기건중탕은 면역관련세포인 microglial cell에서 염증관련 인자들의 분비 및 생성을 억제를 통하여 면역관련 상태를 개선시키는 것으로 사료된다.

참고문헌 (63)

  1. "DongEuiBoGam", pp.260~731 (1999), Seoul:BupIn Publishes Co
  2. "A Literature study of atopic dermatitis for children", 14(2), pp.176~182 (2000)
  3. "Biology of nitric oxide signaling", 28, pp.37~1875 (2000)
  4. "Interleukin-1", 8, pp.253~625 (1997)
  5. "Recent progress in the tumor necrosis factor-alpha fiele", 111, pp.199~209 (1996)
  6. "The effects of Hwangkikunjungtang and GamiHwangkikunjungtang on the recovery of exercise induced fatigue", 18(2), pp.155~166 (1997)
  7. "Inhibitory Effects on the Allergic Reaction of Hwanggigunjung-Tang", 17(5), pp.1293~1298 (2003)
  8. "Inhibition of inducible nitric oxide synthase expression by baicalein in endotoxin", cytokine-stimulated microglia, 2004, pp.67, 957~965
  9. "Determination of nitrite and nitrate by the Griess reaction", pp.491~497 (1996), John Wiley, Sons Ltd
  10. "iNOS-mediated nitric oxide production and its regulation", 75, pp.639~653 (2004)
  11. "Nitric oxide synthases: structure, function and inhibition", 357, pp.593~615 (2001)
  12. "Inflammatory markers of lower respiratory tract infection in elderly people", pp.299~302 (1994)
  13. "Expression and regulation of cyclooxygenase-2 in rat microglia", 243, pp.726~731 (1997)
  14. "Synergistic inhibitory effects of ascorbic acid and acetylsalicylic acid on prostaglandin E2 release in primary rat microglia", 86, pp.173~178 (2003)
  15. "Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon g and bacterial lipopolysaccharide", pp.1779~84 (1993)
  16. "Induction of iNOS expression in skeletal muscle by IL-1β and NF-kB activation: an in vitro and in vivo study", 54, pp.95~104 (2002)
  17. "Proinflammatory and immunoregulatory functions of interleukin-12", 16, pp.365~396 (1998)
  18. "Clinical Application of Oriental Medicine" (1982)
  19. "Molecular mechanisms of endotoxin activity", 164, pp.383~389 (1995)
  20. "Nitric oxide and macrophage function", 15, pp.323~350 (1997)
  21. "(-)-Epigallocatechin-3-gallate blocks the induction of nitric oxide synthase by down-regulating lipopolysaccharide-induced activity of transcription factor nuclear factor-kappaB", 52, pp.465~472 (1997)
  22. "Macrophage oxidation of L-arginine to nitrite and nitrate: Nitrite oxide is an intermediate", 27, pp.8706~8711 (1998)
  23. "Purification of nitric oxide synthase from rat macrophages", pp.12544~7 (1991)
  24. "Altered pain perception and inflammatory response in mice lacking prostacyclin receptor", 388, pp.678~682 (1997)
  25. "Interactions between respiratory epithelial cells and cytokines relationships to lung inflammation", pp.128~45 (1994)
  26. "Regulation of fibroblast cyclooxygenase synthesis by interleukin-1", 263, pp.3022~3028 (1998)
  27. "Cyclooxygenase in biology and disease", 12, pp.1063~1073 (1998)
  28. "Cyclooxygenases : structural, cellular, and molecular biology", 69, pp.145~182 (2000)
  29. "Interleukin-1b induces prostaglandin G", H synthase-2 (cyclooxygenase-2) in primary murine astrocyte cultures, 1996, pp.66, 2532~2540
  30. "Induction of prostanoid biosynthesis by bacterial lipopolysaccharide and isoproterenol in rat microglial cultures", 65, pp.2690~2698 (1995)
  31. "Inflammation and Alzheimer's disease", 21, pp.383~421 (2000)
  32. "Wide therapeutic time window for nimesulide neuroprotection in a model of transient focal cerebral ischemia in the rat", 1007, pp.98~108 (2004)
  33. -2 and inducible nitric oxide synthase in mouse BV2 microglial cells, , 2005, 449~454
  34. "Taming TNF: strategies to restrain this proinflammatory cytokine", 18, pp.487~492 (1997)
  35. "Synergism between tumor necrosis factor-alpha and interferon-gamma on macrophage activation for the killing of intracellular Trypanosoma cruzi through a nitric oxide-dependent mechanism", fres immu(m l), pp.301~7 (1992)
  36. "Tumor necrosis factor-alpha regulates in vivonitric oxide synthesis and induces liver injury during endotoxemia", pp.1055~60 (1994)
  37. "Regulation of interleukin-12 production in antigen-presenting cells", 79, pp.55~92 (2001)
  38. "The role of neuroinflammation and neuroimmune activation in persistent pain", 90, pp.16~ (2001)
  39. "Proinflammatory cytokines and glial cells: their role in neuropathic pain, in Cytokines and Pain (Watkins LR and Maier SF)", pp.159~181 (1999), Birkhauser Verlag, Basel
  40. "Isoforms of Jun kinase are differentially expressed and activated in human monocyte", macrophage (THP-1) cells, 2001, pp.166, 5646~5653
  41. "NF-kB and Rel proteins: evolutionarily conserved mediators of immune responses", 16, pp.225~260 (1998)
  42. "Nitric oxide and superoxide in inflammation and immune regulation", 55, pp.469~487 (2003)
  43. "Catalase induced expression of inflammatory mediators via activation of NF-nB, PI3K", AKT, p70S6K, and JNKs in BV2 microglia, 2005, pp.17, 625~633
  44. "The p38 MAP kinase pathway as a therapeutic target in inflammatory disease", 4, pp.372~377 (2004)
  45. "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases", 298, pp.1911~1912 (2002)
  46. "The NF-B and I-B proteins: new discoveries and insights", pp.649~681 (1996)
  47. "Curcumin Suppresses Lipopolysaccharide-Induced Cyclooxygenase -2 Expressionby Inhibiting Activator Protein 1 and Nuclear Factor B Bindings in BV2 Microglial Cells", 94, pp.325~328 (2004)
  48. "The I kappa B kinase (IKK) and NF-kappa B: key elements of proinflammatory signalling", 12, pp.85~98 (2000)
  49. B activity, , 2000, 621~663
  50. "The radioprotective effects of bu-zhong-yi-qi-tang a prescription of traditional Chinese medicine", 30(1), pp.127~137 (2002)
  51. "Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation", 81, pp.807~869 (2001)
  52. "The effects of Radix Puerariae decoction on levels of antibody and interleukin-2 in mice", 25(10), pp.731~732 (2002)
  53. "a mediator of lipopolysaccharide effects", immu(biol), pp.233~56 (1993)
  54. "Macrophages and microglia in the nervous system", pp.273~7 (1988)
  55. "Anti-hyperalgesic and morphine sparing actions of propentofylline following nerve injury in rats: mechanistic implications of spinal glia and proinflammatory cytokines", 104(3), pp.655~664 (2003)
  56. "Inhibition of Microglial Activation Attenuates the Development but Not Existing Hypersensitivity in a Rat Model of Neuropathy", 306, pp.624~630 (2003)
  57. "Assays of cell growth and cytotoxicity", pp.180~ (1998), New York:Oxford University press
  58. "Vitamin E (a-tocopherol) attenuates cyclo-oxygenase 2 transcription and synthesis in immortalized murine BV2 microglia", 370, pp.459~467 (2003)
  59. "Evidence for the involvement of spinal cord glia in subcutaneous formalin induced hyperalgesia in the rat", 71, pp.225~235 (1997)
  60. "Spinal cord glia: new players in pain", 93, pp.201~205 (2001)
  61. "Role of transcription factor NF-B", Rel in induction of nitric oxide synthase, 1994;269, 4705~8
  62. "Lipopolysaccharide induction of the tumor necrosis factor-alpha promoter in human monocytic cells. Regulation by Egr-1, c-Jun, and NF-kappaB transcription factors", 272, pp.17795~17801 (1997)
  63. "Hideaki MATSUDA, and Michinori KUBO. Antipruritic and Antiinflammatory Effects of Aqueous Extract from Si-Wu-Tang", 25(9), pp.1175~1178 (2002)

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