Regulatory Effect of Gigukjihwangtanggami on Cytokine Production in Patients with Cerebral Infarction
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Abstract
The Korean genuine medicine, “Gigukjihwangtanggami (GJT)” has long been used clinically for hypertension and various cerebrovascular diseases. However, experimental study has been carried out very little. Recently cytokines involved in the regulation of inflammatory reactions and immune responses may play an important role in the pathogenesis of cerebral infarction (CI). The aim of this study is to investigate the effect of GJT on the production of pro-inflammatory cytokines and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMCs) stimulated with lipopolysaccaride (LPS) from CI patients. The amount of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, IL-8, IL-4 and IL-10 in PBMC culture supernatant was significantly increased in the LPS treated cells compared to unstimulated cells. GJT inhibited the production of TNF-α, IL-1β, IL-6 and IL-8 induced by LPS. The maximal inhibition rate of the TNF-α, IL-1β, IL-6 and IL-8 production by pretreatment of GJT (1 mg/ml) was 57.32 ± 2.5% (P<0.05), 42.02 ± 3.5% (P<0.05), 40.02 ± 2.3% (P<0.05) and 48.02 ± 3.1% (P<0.05), respectively. In the other hand, GJT increased the production of IL-4 and IL-10. The maximal increase rate of the IL-4 and IL-10 production by pretreatment of GJT (1 mg/ml) was 42.4 ± 3.3% (P<0.05) and 56.4 ± 2.9% (P<0.05), respectively. Taken together, these results indicate that GJT may have regulatory effects on the cytokine production and suggest that GJT might use clinically for the treatment of CI.
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