Effects of the Aqueous Extract of Rehmanniae Radix Preparata onLipopolysaccharide-induced Expressions of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in Mouse BV2 Microglial Cells
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Rehmanniae radix preparata is the root of Rehmanniae glutinosa Liboschitz var. purpurea Makino which has been classified into Scrophulariaceae. Rehmanniae radix preparata has been used for the treatment of diabetes, for the relief of the pain, and for the anti-oxidative action. In this study, the effect of the aqueous extract of Rehmanniae radix preparata on lipopolysaccharide-induced inflammation was investigated by using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot, prostaglandin E2 immunoassay, and nitric oxide (NO) detection in mouse BV2 microglial cells. In the present results, the aqueous extract of Rehmanniae radix preparata suppressed prostaglandin E2 (PGE2) synthesis and nitric oxide production by inhibiting the lipopolysaccharide-stimulated expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) mRNA and protein in mouse BV2 cells. These results show that Rehmanniae radix preparata exerts anti-inflammatory effect probably by suppressing of COX-2 and iNOS expressions.
Abstract
Rehmanniae radix preparata is the root of Rehmanniae glutinosa Liboschitz var. purpurea Makino which has been classified into Scrophulariaceae. Rehmanniae radix preparata has been used for the treatment of diabetes, for the relief of the pain, and for the anti-oxidative action. In this study, the effect of the aqueous extract of Rehmanniae radix preparata on lipopolysaccharide-induced inflammation was investigated by using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot, prostaglandin E2 immunoassay, and nitric oxide (NO) detection in mouse BV2 microglial cells. In the present results, the aqueous extract of Rehmanniae radix preparata suppressed prostaglandin E2 (PGE2) synthesis and nitric oxide production by inhibiting the lipopolysaccharide-stimulated expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) mRNA and protein in mouse BV2 cells. These results show that Rehmanniae radix preparata exerts anti-inflammatory effect probably by suppressing of COX-2 and iNOS expressions.
참고문헌 (19)
- Gonzalez-Scarano, F.,, "Microglia as mediators of inflammatory and degenerative diseases", gonzalez-scara, pp.219~240 (1999)
- Min, K.J.,, "Protein kinase A mediates microglial activation induced by plasminogen and gangliosides", pp.461~467 (2004)
- Kim, W.K.,, "TGF-α represses activation and resultant death of microglia via inhibition of phosphatidylinositol 3-kinase activity", j immu(l 172), pp.7015~7023 (2004)
- Kubes, P.,, "Nitric oxide and intestinal inflammation", pp.150~158 (2000)
- Mitchell, J.A.,, "Cyclooxygenase-2: regulation and relevance in inflammation.", pp.1535~2 1542 (1995)
- Crofford, L.J.,, "Basic biology and clinical application of specific cyclooxygenase-2 inhibitors", pp.4~13 (2000)
- Dawson, "A novel neuronal messenger molecule in brain", pp.297~311 (1992)
- Hattori, "Purification of nitric oxide synthase from rat macrophages", pp.12544~12547 (1991)
- Jeong, "Comparative studies on the antidiabetic activities of Rehmannia Radices", pp.22~31 (1990)
- Jo, S.I, "Anti oxidative effects of RADIX REHMANNIAE PREPARATA on toxic agent induced kidney cell injury", 18, pp.119~126 (2003)
- Kim, H.M.,, "Rehmannia glutinosa inhibits tumor necrosis factor-alpha and interleukin-1 secretion from mouse astrocytes", pp.171~176 (1999)
- McGeer, P.L.,, "The inflammatory response system of brain implications for therapy of Alzheimer and other neurodegenerative diseases" (1995)
- Watkins, L.R.,, "The pain of being sick implications of immune-to-brain communication for understanding pain", pp.29~57 (2000)
- Mitchell, J.A.,, "regulation and relevance in inflammation", pp.1535~2 1542 (1995)
- Hinz, B.,, "upregulates cyclooxygenase-2 expression in lipopolysaccharide- stimulated RAW 264", pp.7 744~748 (2000)
- Vane, "Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation", pp.2046~2050 (1994)
- Szabo,C, "Alternations in nitric oxide production in various forms of circulatory shock", 3, pp.2~32 (1995)
- Hyun, J.W.,, "The cytotoxic activity of sterol derivatives from Pulsatilla chinensis Regal", pp.145~150 (1996)
- Hyun, J.W.,, "The cytotoxic activity of sterol derivatives from Pulsatilla chinensis Regal", pp.145~150 (1996)