본문 바로가기
KERD
논문KCI 등재

창출 추출물의 BV2 cell 소염작용에 관한 실험적 연구

Anti-inflammatory effect of various solvent extract from Atractylodes japonica on Lipopolysaccharide-induced Inflammation in BV2 cells.

김경준|심성용|허인희
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2007.08|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제20권 제2호|pp. 36-46

1

조회

0

다운로드

10

인용

0

참고문헌

주제분류한의학

초록

Abstract

Objective : In this study, the effect of Atractylodes japonica against LPS induced inflammation in mouse microglia BV2 cells was investigated. Method : Microglia BV2 Cells viability was determined using the MTT assay. We used water, ethanol extract from Atractylodes japonica and studied on the anti-inflammatory effect of lipopolysaccharide-induced inflammation using reverse transcription polymerase chain reaction (RT-PCR), western blot, and nitric oxide detection on mouse microglia BV2 cells. Result : The MTT assay revealed that it's extract has no significant cytotoxicity in the microglia BV2 cell. Various solvent extract from Atractylodes japonica inhibited nitrite production, iNOS protein and mRNA expression levels. And also it's extracts significantly reduced lipopolysaccharide-induced COX-2 activation in RT-PCR and western blot in lipopolysaccharide-induced microglia BV2 cells Conclusion : In this study, it's extracts was shown to suppress NO production by inhibiting iNOS expression and COX-2 activity. With this effects of anti-inflammation, we suggests that, it's extracts may be a useful candidate for the development of a drug on the related inflammatory diseases in brain.

참고문헌 (34)

  1. "Assessment of a topical NSAIDs in the treatment of pain and inflammation", 33(14-C2), pp.10~13 (2004)
  2. "Induction and activation by zinc of NADPH oxidase in cultured cortical neurons and astrocytes", RC111, pp.1~5 (2000)
  3. "Protein oxidation in aging, disease, and oxidative stress", 272, pp.20313~20316 (1997)
  4. "Vascular endothelial cells synthesize nitric oxide from L-argine", 333, pp.664~666 (1998)
  5. "Microglia: a sensor for pathological events in the CNS", 19, pp.312~318 (1996)
  6. "Microglia as effector cells in brain damage and repair focus on prostanoids and nitric oxide", 54, pp.99~125 (1998)
  7. "Suppression of inducible nitric oxide synthase and cyclooxygenase-2 in downregulating nuclear factor-kappa B pathway by Garcinol", 41(3), pp.140~149 (2004)
  8. "Dual inhibition oh nitric oxide and prostaglandin prodiction contributes to the antiinflammatory properties of nitric axide synthase inhibitor", 96, pp.301~308 (1995)
  9. "加味蒼栢散이 Carrageenan유발 동물모델 관절염의 소염, 진통, 해열 및 면역에 미치는 영향", 15(1), pp.127~141 (2005)
  10. "蒼朮에 대한 文獻的 考察", 7(1), pp.583~589 (1997)
  11. "한국산 삽주의 기능성", 8(1), pp.86~91 (2001)
  12. "The effect of crude drugs using diuretic on horse kidney", -ade(sine triphosphatase), pp.138~45 (1991)
  13. "삽주(Atractylodes japonica Koidzumi ex Kitamura)의 定量的 形質에 의한 변이분석", 15(1), pp.36~42 (2002)
  14. "Effect of Methanol Extract of Rubus Coreanus Miquel and Atractylodes Japonica Koidzumi on Hepatic Toxicity and Immunomodulating Activity in Mice", 9(2), pp.68~73 (2006)
  15. "창출성분의 장관 면역 자극을 통한 골수세포 증식 활성", 115, pp.135~141 (2001)
  16. "Inducible nitric oxide synthase expression in activated rat microglial cultures is downregulated by exogenous prostaglandin E2 and by cyclooxygenase inhibitors", 19, pp.152~160 (1997)
  17. "Annual Review of Medicine" (1987)
  18. "Silymarin protects dopaminergic neurons against lipopolysaccharide-induced neurotoxicity by inhibiting microglia activation", 16, pp.2103~2112 (2002)
  19. "Evaluation of natural products on inhibition of inducible cyclooxygen(COX-2) and nitric oxide synthase(iNOS) in cultured mouse macrophage cells", 83, pp.153~159 (2002)
  20. "Protein kinase C-mediated regulation of inducible nitric oxide synthase expression in cultured microglial cells", 92, pp.170~178 (1998)
  21. "Regulation of biosynthesis of nitric oxide"
  22. "五味消毒飮이 마우스 복강 재식세포에서 NO의 분비과정에 미치는 영향", 16(2), pp.921~927 (2002)
  23. "Complex regulation of human inducible nitric oxide synthase gene transcription by Stat1 and NF-kB", 98, pp.8638~8643 (2001)
  24. "Nitric oxide up-regulates the release of inflammatory mediators by mouse macrophages", 25, pp.947~951 (1995)
  25. "Microglia as a source and target of cytokines", 40, pp.140~155 (2002)
  26. "Quercetin, but not rutin and quercetin, prevention of H2O2-induced apoptosis via anti-oxidant activity and heme oxygenase 1 gene expression in macrophages", 69, pp.1839~1851 (2005)
  27. "Distinct isoforms(COX-1 and COX-2) of cyclocxygenase: Possible physiological and therapeutic implications", 10, pp.1~15 (1996)
  28. "Expression and regulation of cyclooxygenase-2 in rat microglia", 243, pp.726~731 (1997)
  29. "Prostaglandin E(2) upregulates cyclooxygenase-2 expression in lipopolysaccharide-stimulated RAW 264.7 macrophages", 227, pp.744~748 (2000)
  30. "Basic biology and clinical application of cyclooxygenase-2 inhibitors", 43(1), pp.4~13 (2000)
  31. "Protein oxidation in aging, disease, and oxidative stress", 1997, pp.20313~20316
  32. "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", 299, pp.152~178 (1999)
  33. "Exposure to hydrogen peroxide induces cell death via apoptosis in cultured rat cortical neurons" (1994)
  34. "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", 299, pp.152~178 (1999)

관련 논문AI추천

大薊와 실리비닌의 Mouse BV2 Microglial Cells에서 Lipopolysaccharide에 의해 유발된 염증반응에 대한 신경보호 효과김동우, 박종형, 여현수, 전찬용, 최유경대한한방내과학회 · 대한한방내과학회지2007 · 제28권 제1호삽주 육성품종 뿌리의 항산화 및 항염증 효과 비교김동휘, 박춘근, 이승은, 이정훈, 장귀영, 장재기, 정진태, 정현수, 최재훈한국약용작물학회 · 한국약용작물학회지2018 · 제26권 제5호Inhibitory Effects of Jojoongikgi-tang on the secretion of PGE2 and NO and Cytokines in LPS-stimulated BV2 microglial Cells고우신, 김보경, 유선애, 이승연, 이준문대한한방소아과학회 · 대한한방소아과학회지2007 · 제21권 제2호Anti-inflammatory Effects of Gastrodia elata Extract in Lipopolysaccharide-stimulated BV-2 Microglia Cell김재규, 서영경, 이준혁, 장지연, 최나현, 최병태, 허영현한의병리학회 · 동의생리병리학회지2012 · 제26권 제5호豬苓車前子湯이 βA와 LPS로 처리된 BV2 microglial cell에 미치는 영향류창희, 이상룡, 정인철대한한방신경정신과학회 · 동의신경정신과학회지2012 · 제23권 제1호木天蓼子가 LPS로 유되된 Mouse BV2 Microglial cells의 염증반응에 미치는 영향김기태한의병리학회 · 동의생리병리학회지2022 · 제36권 제4호

이 간행물의 다른 논문 (21건)

싸리꽃 에탄올 추출물의 皮膚 生理活性 효과이성진, 김영목, 유인식, 이장천, 임규상, 문연자, 우원홍, 이성원pp. 1-9五倍散加味가 粘膜 및 皮膚疾患에 미치는 영향노석선, 홍석훈pp. 10-35加味大黃膏의 멜라닌세포 활성억제가 자외선 조사로 인한 피부 손상 완화에 미치는 영향홍승욱, 이현우pp. 47-67硫黃이 여드름 유발균과 염증에 미치는 영향이선용, 서형식pp. 68-76如意金黃散이 여드름 유발균과 염증에 미치는 영향서형식, 유진곤pp. 77-88
원문 보기