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12주간 복합운동이 난소절제 쥐 골밀도, 골대사 지표 및 OPG/RANKL mRNA 발현에 미치는 영향

Effects of 12 weeks combined exercise on BMD, bone metabolism markers and OPG/RANKL mRNA expression of bone marrow cell in ovariectomized rats

김상배|윤진환|지용석|박세환
국민체육진흥공단 한국스포츠과학원 (Korea Institute of Sport Science)|2019.09|체육과학연구 (Korean Journal of Sport Science)|제30권 제3호|pp. 449-458|DOI 10.24985/kjss.2019.30.3.449

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참고문헌

키워드

골다공증Osteoporosis골밀도Bone mineral density복합운동OPGCombined exercise난소절제RANKL mRNAOvariectomized rats
주제분류체육

초록

[목적] 골다공증은 점진적인 골량 감소 및 골 미세구조의 손상으로 특징되는 대사성 골 질환이다. 특히, 폐경기성 골다공증은 폐경 후 중년 여성에게서 흔히 발생된다. 이 연구의 목적은 난소절제 쥐를 대상으로 12주간의 복합운동이 골밀도, 골대사 지표 및 OPG/RANKL mRNA 발현에 미치는 영향을 규명하고자 하였다. [방법] 총 40마리 SD계열 암컷 쥐를 통제군(CON, n=10), 난소절제군(OVX, n=10), 난소절제-저항성 운동군(OVX-REX, n=10), 난소절제-복합운동군(OVX-ARE, n=10)으로 무선배정 하였다. 복합운동은 트레드밀 운동과 사다리 등반 운동을 격일제로 12주 동안 주 4회 실시하였다. [결과] 난소절제군과 비교하여 모든 운동 처치군의 골밀도 및 골강도는 유의하게 높은 것으로 나타났다(p<0.05). 골 대사 지표에서, 혈청 CTX-1 농도는 난소절제군과 비교하여 모든 운동 처치군에서 유의한 감소(p<0.05)가 나타났으며, 혈청 osteocalcin 농도는 유의하게 증가하였다(p<0.05). 추가적으로 OPG mRNA 발현은 운동 처치군이 난소절제군과 비교하여 유의한 증가를 나타내었고, 반면, RANKL mRNA 발현 수준은 감소하는 경향을 보였으나, 그룹 간 유의한 차이는 없었다. 특히, 복합운동군이 단일 형태의 저항성 운동군과 비교하여 모든 측정 변인에서 더 긍정적인 효과를 나타내었다. [결론] 이상의 결과를 종합해 볼 때, 복합운동은 폐경기성 골다공증의 지연 및 예방을 위한 더 효율적인 운동 중재 전략으로 판단된다.

Abstract

[Purpose] Osteoporosis is a systemic metabolic bone disease characterized by gradual decrease of bone mass and damage of the bone microstructure. In particular, postmenopausal osteoporosis is the most common type in women after menopause. This study aims to investigate the effects of combined exercise training on bone mineral density (BMD) and OPG/RANKL mRNA levels in ovariectomized rats. [Method] A total of 40 Sprague-Dawley female rats were randomly divided into four groups: (1) CON (sham-operation, n=10), (2) OVX (ovariectomy, n=10), (3) OVX-REX (ovariectomy-resistance exercise, n=10), and (4) OVX-ARE (ovariectomy-combined aerobic and resistance exercise, n=10). Combined exercise training was performed on a treadmill and ladder adapted to rats in alternate days (4 days/wk, for 12 wk). [Results] Compared to the OVX group, all exercise treatments increased BMD and bone breaking force(p<0.05). In the bone turnover markers, serum C-terminal telopeptides of type-1 collagen (CTX-1) was significantly decreased in the exercise groups compared with OVX group and osteocalcin (OC) level was increased in the exercise groups (p<0.05). Additionally, in the exercise groups, expression of OPG mRNA was significantly increased compared with OVX group (p<0.05), and RANKL mRNA was slightly decreased but no significant between groups. Furthermore, OVX-ARE group showed more effects than OVX-REX group. [Conclusions] These results suggest that combined exercise may be a more effective therapeutic strategy to prevent and delay postmenopausal osteoporosis than resistance-only training.

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