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운동강도의 차이가 고령 골감소증 여성의 골대사지표 및 RANKL/ RANK/OPG 시스템 사이토카인에 미치는 영향

The Effects of Exercise Intensity Difference on Bone Metabolic Markers and Cytokines of the RANKL/RANK/OPG System in Korean Osteopenia Elderly Women

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PURPOSE: Regular exercise augments the bone mineral density in postmenopausal elderly women. However, its acute effects by different intensities on bone metabolic markers and cytokines of the RANKL/RANK/OPG system have not been determined. Our aim was to investigate the effects exercise intensity difference on these markers and RANKL/RANK/OPG system in Korean osteopenia elderly women. METHODS: Twelve osteopenia elderly women (T-score -2.46±0.48; aged 68.17±3.04 years) examined these markers and RANKL/ RANK/OPG system responses to acute high (80% VO2max ; HIGH group), low (40% VO2max; LOW group) intensity with a cycle ergometer, and sedentary rest (CON group) in a randomized, cross-over repeated measures design. The concentrations of bone metabolic markers and cytokines measured before, immediately after and then recovery 60 minutes after exercise. mRNA expressions of RANKL & OPG from PBMC were also measured at same times. RESULTS: There were no significant changes in the serum CTx and RANKL, and mRNA expression of RNAKL, indicating bone resorption (NS). On the other hand, although the serum OPG showed significant change in high intensity (p=.027; group×time), the serum OC showed significant change in low intensity (p=.044; group×time), indicating bone formation, respectively. CONCLUSIONS: These results suggest that a single bout exercise might effect on bone formation alone with OPG responds to high intensity, OC responds to low intensity. Additional studies are needed on the difference in change between OC and OPG due to exercise intensity.

Abstract

PURPOSE: Regular exercise augments the bone mineral density in postmenopausal elderly women. However, its acute effects by different intensities on bone metabolic markers and cytokines of the RANKL/RANK/OPG system have not been determined. Our aim was to investigate the effects exercise intensity difference on these markers and RANKL/RANK/OPG system in Korean osteopenia elderly women. METHODS: Twelve osteopenia elderly women (T-score -2.46±0.48; aged 68.17±3.04 years) examined these markers and RANKL/ RANK/OPG system responses to acute high (80% VO2max ; HIGH group), low (40% VO2max; LOW group) intensity with a cycle ergometer, and sedentary rest (CON group) in a randomized, cross-over repeated measures design. The concentrations of bone metabolic markers and cytokines measured before, immediately after and then recovery 60 minutes after exercise. mRNA expressions of RANKL & OPG from PBMC were also measured at same times. RESULTS: There were no significant changes in the serum CTx and RANKL, and mRNA expression of RNAKL, indicating bone resorption (NS). On the other hand, although the serum OPG showed significant change in high intensity (p=.027; group×time), the serum OC showed significant change in low intensity (p=.044; group×time), indicating bone formation, respectively. CONCLUSIONS: These results suggest that a single bout exercise might effect on bone formation alone with OPG responds to high intensity, OC responds to low intensity. Additional studies are needed on the difference in change between OC and OPG due to exercise intensity.

참고문헌 (56)

  1. JungEun Kim, "The effects of exercise training and type of exercise training on changes in bone mineral denstiy in Korean postmenopausal women: a systematic review", Journal of Exercise Nutrition & Biochemistry, 20(3), pp.7~15 (2016), 한국운동영양학회
  2. Woźniak-Holecka J, "Nutritional education in the primary prevention of osteoporosis in perimenopausal and postmenopausal women", Prz Menopauzalny, 13(1), pp.56~63 (2014)
  3. Morris HS, "Piezoelectric Effect in Bone", Nature, 197(4862), pp.81~ (1963)
  4. Voss LA, "Exercise-induced loss of bone density in athletes", Journal of the American Academy of Orthopaedic Surgeons, 6(6), pp.349~357 (1998)
  5. Bilanin JE, "Lower vertebral bone density in male long distance runners", Medicine & Science in Sports & Exercise, 21(1), pp.66~70 (1989)
  6. Risser WL, "Bone hort-term step aerobics exercise on bone metabolism and functional fitness in postmenopausal women with low bone mass", Osteoporosis International (2016)
  7. Wen HJ, "Effects of short-term step aerobics exercise on bone metabolism and functional fitness in postmenopausal women with low bone mass", Osteoporos Int (2016)
  8. Chen X, "Treadmill running exercise prevents senile osteoporosis and upregulates the Wnt signaling pathway in SAMP6 mice", Oncotarget, 7(44), pp.71072~71086 (2016)
  9. Yamamoto T, "Mechanical stress induces expression of cytokines in human periodontal ligament cells", Oral disease, 12(2), pp.171~175 (2006)
  10. Xiong Y, "Identification of two groups of smoldering multiple myeloma patients who are either high or low producers of interleukin-1", Journal of Interferon & Cytokine Research, 26(2), pp.83~95 (2006)
  11. Giannini S, "Bone disease in primary hypercalciuria", Critical Reviews in Clinical Laboratory Sciences, 42(3), pp.229~248 (2005)
  12. Inanir A, "The effects of calcitriol therapy on serum interleukin-1, interleukin-6 and tumour necrosis factor-alpha concentrations in post-menopausal patients with osteoporosis", Journal of International Medical Research, 32(6), pp.570~582 (2004)
  13. Cheng J, "Molecular mechanisms of the biphasic effects of interferon-γ on osteoclastogenesis", Journal of Interferon & Cytokine Research, 32(1), pp.34~45 (2012)
  14. Kotake S, "IFN-gamma-producing human T cells directly induce osteoclastogenesis from human monocytes via the expression of RANKL", European Journal of Immunology, 35(11), pp.3353~3363 (2005)
  15. 김창선, "일회성 운동이 골감소증 고령여성의 골대사성 사이토카인 생성에미치는 영향", 운동과학, 25(4), pp.274~281 (2016), 한국운동생리학회[DOI]
  16. 김효진, "The effects of pilates exercise on lipid metabolism and inflammatory cytokines mRNA expression in female undergraduates", Journal of Exercise Nutrition & Biochemistry, 18(3), pp.267~275 (2014), 한국운동영양학회
  17. 김창선, "The effects of a single bout pilates exercise on mRNA expression of bone metabolic cytokines in osteopenia women", Journal of Exercise Nutrition & Biochemistry, 18(1), pp.69~78 (2014), 한국운동영양학회
  18. 김창선, "Expression of bone-resorbing cytokines according to aerobic exercise intensities", Journal of Exercise Nutrition & Biochemistry, 15(3), pp.131~139 (2011), 한국운동영양학회
  19. Lacey DL, "Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation", Cell Press, 93(2), pp.165~176 (1998), Cell Press
  20. Yasuda H, "Osteoclast differentiation factor is a ligand for osteoprotegerin", osteoclastogenesis-inhibitory factor and is identical to TRANCE, Proceedings of the National Academy of Science of the USA, pp.1998, 95(7)[DOI]
  21. Cenci S, "Estrogen-deficiency induces bone loss by enhancing T-cell production of TNF-α", Journal of Clinical Investigation, 106(10), pp.1229~1327 (2000)
  22. Kanematsu M, "Prostaglandin E2 induces expression of receptor activator of nuclear factorkB ligand", osteoprotegerin ligand on pre-B cells : implications for accelerated osteoclastogenesis in estrogen deficiency, 2000, pp.15(7), 1321~1329
  23. Simonet WS, "Osteoprotegerin : a novel secreted protein involved in the regulation of bone density", pp.309~319 (1997), Cell Press
  24. Power MJ, "Osteocalcin : diagnostic methods and clinical applications", Critical Reviews in Clinical Laboratory Sciences, 28(4), pp.287~335 (1991)
  25. Christenson RH, "Biochemical markers of bone metabolism : an overview", Clinical Biochemistry, 30(8), pp.573~593 (1997)
  26. Bonde M, "Measurement of bone degradation products in serum using antibodies reactive with an isomerized from of an 8 amino acid sequence of the C-telopeptide of type I collagen", Journal of Bone and Mineral Research, 12(7), pp.1028~1034 (1997)
  27. Garnero P, "Increased bone turnover in late postmenopausal women is a major determinant of osteoporosis", Journal of Bone and Mineral Research, 11(3), pp.337~349 (1996)
  28. Gołąbek K., "Association between omentin-1, bone metabolism markers, and cytokines of the RANKL", RANK, Endokrynologia Polska, pp.2015, 66(6)[DOI]
  29. Kanis JA, "The diagnosis of osteoporosis", Journal of Bone and Mineral Research, 9(8), pp.1137~1141 (1994)
  30. Pacifici R, "Bone matrix constituents stimulate interleukin-1 release from human blood mononuclear cells", Journal of Clinical Investigation, 87(1), pp.221~228 (1991)
  31. Chomczynski P, "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction", Analytical Biochemistry, 162(1), pp.156~159 (1987)
  32. Kong YY, "OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymphnode organogenesis", Nature, 397, pp.315~323 (1999)
  33. Mosheimer BA, "Syndecan-1 is involved in osteoprotegerin-induced chemotaxis in human peripheral blood monocytes", The Journal of Clinical Endocrinology & Metabolism, 90(5), pp.2964~2971 (2005)
  34. Kim NS, "Receptor activator of NF-Kappa B ligand regulates the proliferation of mammary epithelial cells via Id2", Molecular and Cellular Biology, 26(3), pp.1002~1013 (2006)
  35. Asagiri M, "The molecular understanding of osteoclast differentiation", Bone, 40(2), pp.251~264 (2007)
  36. Wada T, "RANKL-RANK signaling in osteoclastogenesis and bone disease", Trends in molecular medicine, 12(1), pp.17~25 (2006)
  37. Takayanagi H, "Osteoimmunology : shared mechanisms and crosstalk between the immune and bone systems", Nature reviews Immunology, 7(4), pp.292~304 (2007)
  38. Eghbali-Fatourechi G, "Role of RANK ligand in mediating increased bone resorption in early postmenopausal women", Journal of Clinical Investigation, 111(8), pp.1221~1230 (2003)
  39. Hofbauer LC, "Osteoprotegerin ligand and osteoprotegerin : new concepts of the pathogenesis and therapy of metabolic bone diseases", Dtsch MedWochenschr, 126(6), pp.145~150 (2001)
  40. O’Brien EA, "Osteoprotegerin is produced when prostaglandin synthesis is inhibited causing osteoclasts to detach from the surface of mouse parietal bone and attach to the endocranial membrane", Bone, 28(2), pp.208~214 (2001)
  41. Pacifici R, "Bone matrix constituents stimulate interleukin-1 release from human blood mononuclear cells", Journal of Clinical Investigation, 87(1), pp.221~228 (1991)
  42. Olivier BJ, "Increased osteoclast formation and activity by peripheral blood mononuclear cells in chronic liver disease patients with osteopenia", Hepatology, 47(1), pp.259~267 (2008)
  43. Bashir A, "Changes in RANKL", OPG, Steroids, pp.2005, 170(13)[DOI]
  44. Stuss M, "Assessment of OPG", RANK, The Journal of Clinical Endocrinology & Metabolism, pp.2013, 98(5)[DOI]
  45. Hofbauer LC, "Role of receptor activator of nuclear factor-kappaB ligand and osteoprotegerin in bone cell biology", Journal of Molecular Medicine, 79(5-6), pp.243~253 (2001)
  46. Chmielnicka M, "The influence of corticosteroid treatment on the OPG", RANK, Postepy Dermatol Alergol, pp.2015, 31(5)[DOI]
  47. Kearns AE, "Receptor activator of nuclear factor kappaB ligand and osteoprotegerin regulation of bone remodeling in health and disease", Endocrine Reviews, 29(2), pp.155~192 (2008)
  48. Silva I, "RANK", RANKL, Acta Reumatol Port, pp.2011, 36(3)[DOI]
  49. Munoz-Calvo MT, "Maintained malnutrition produces a progressive decrease in OPG", RANKL ratio and leptin levels in patients with anorexia nervosa, 2007, pp.67(4), 387~393
  50. Ostrowska Z, "RANKL", RANK, Bone, pp.2012, 50(1)[DOI]
  51. Gombos GC, "Direct effects of physical training on markers of bone metabolism and serum sclerostin concentrations in older adults with low bone mass", BMC Musculoskeletal Disorders, 17(254), pp.1~8 (2016)
  52. Vanessa D Sherk, "Acute Bone Marker Responses to Whole-Body Vibration and Resistance Exercise in Young Women", Journal of Clinical Densitometry, 16(1), pp.104~109 (2013)
  53. Mera P, "Osteocalcin Signaling in Myofibers Is Necessary and Sufficient for Optimum Adaptation to Exercise", Cell Metabolism, 23(6), pp.1078~1092 (2016)
  54. Levinger I, "The effect of acute exercise on undercarboxylated osteocalcin and insulin sensitivity in obese men", Journal of Bone and Mineral Research, 29(12), pp.2571~2576 (2014)
  55. Hotta M, "The importance of body weight history in the occurrence and recovery of osteoporosis in patients with anorexia nervosa : evaluation by dual x-ray absorptiometry and bone metabolic markers", European Journal of Endocrinology, 139(3), pp.276~283 (1998)
  56. Weinbrenner T, "Body mass index and disease duration are predictors of disturbed bone turnover in anorexia nervosa. A case-control study", European Journal of Clinical Nutrition, 57(10), pp.1262~1267 (2003)

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