진동운동이 난소절제 쥐의 OPG, RANKL, BMD에 미치는 영향
Effects of WBV Exercise on OPG, RANKL and BMD in Ovariectomized Rats
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초록
신체활동은 골다공증의 예방과 치료를 위해서 권장되고 있다. 이 연구의 목적은 골 소실을 유발시킨 쥐를 대상으로 규칙적인 전신진동운동(45Hz, 0.3g, 5일/주, 12주)을 적용시킨 후, 대퇴골의 골 기질 세포의 단백질 수준에서의 OPG와 RANKL의 발현수준과 대퇴골의 골밀도 측정을 통하여 진동운동이 골 재형성에 미치는 영향을 살펴보고자 하였다. 실험동물은 총 3집단(sham-c, ovx-c, ovx-e)으로 나누었다. 실험을 통해 얻어진 결과는 SPSS 11.0을 이용하여 평균과 표준오차를 산출하고, 집단에 따른 변인의 차이는 one-way ANOVA를 이용하였으며, 유의수준은 p<.05로 설정하였다. 본 연구의 결과는 첫째, 전신진동운동은 난소절제 집단의 OPG 발현을 유의하게 증가시켰다(p<.01). 둘째, 전신진동운동은 난소절제 집단의 RANKL 발현에 유의한 차이가 없었다. 셋째, 전신진동운동은 난소절제 집단의 BMD를 유의하게 증가시켰다(p<.001). 넷째, 전신진동운동은 난소절제 집단의 대퇴골의 골 면적에 유의한 차이가 없었다. 이상과 같은 결과는 진동형태의 물리적 자극이 골다공증 환자의 골 소실을 억제하고, 골 형성 자극을 기대할 수 있는 운동이 될 수 있다고 사료된다.
Abstract
Physical exercise is recommended for the prevention and treatment of osteoporosis. The present study evaluated the influence of WBV(whole body vibration) exercise on the bone remodeling in ovariectomized rats. The aim of this study was to investigate possibility for activation of bone formation and decrease of bone loss while WBV exercise(frequency: 45Hz, vertical acceleration: 0.3g, 5days/wk, for 12wks) was performed. The experimental animals have been divided into 3 groups (sham-c, ovx-c; ovx-e). OPG and RANKL concentration were evaluated from bone marrow stromal cell. DXA measurement of BMD and bone area were from femur bone. All values were mean±SE. To compare the 3 groups statistically, one-way ANOVA was used. Post hoc was Scheffe and statistical significant value was p<.05. Vibration exercise group showed significantly higher OPG concentration(p<.01), BMD(p<.001) than non vibration exercise group in ovariectomized rats. But there were not difference of RANKL concentration and bone area values compared to control group. These present results indicate that WBV exercise is effective method for activating bone formation and preventing early post-ovariectomy bone loss.
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