익모초 에탄올 추출물의 향장소재 연구
A study on Cosmetic Applications of Ethanol Extracts of Leonurus sibiricus L.
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We conducted this study to investigate antioxidant and cytotoxicity of ethanol extracts of Leonurus sibiricus L. Also, we examined the cytoprotected effect of ethanol extracts of Leonurus sibiricus L. about antihemolytic of Sprague Dawley rat red blood cell. After extracting Leonurus sibiricus L. with ethanol. We treated samples by concentration and conducted DPPH radical scavenging activity and SOD-like activity. About human skin fibroblast HS68 cell, through MTT assay, we assessed toxicity of samples. Also, to assess effect of antihemolytic, we observed effect of extracts which we got after inducing oxidation of Sprague Dawley rat red blood cell using H2O2. The result showed that extracts of Leonurus sibiricus L. was effective as concentration of DPPH radical scavenging activity and SOD-like activity increased. After we checked cytotoxicity of Leonurus sibiricus L. using human fibroblast HS68 cell, it showed life rate more than 90 percent at 5~100 μg/ml and we made sure of its safety for skin. In the experiment for assessing of anti-hemolytic, we could observe the group that treated with extracts showed the higher rate of anti-hemolytic than control group that treated with H2O2 only. This result showed by eliminating ROS, extracts of Leonurus sibiricus L. could protect cell membrane and work as antioxidants. These results were that extracts of Leonurus sibiricus L. can eliminate active oxygen specie, have no cytotoxicity in fibroblast cell and show actual effectiveness of cell protection about anti-hemolytic activity in red blood cell, so they show possibility as functional natural product.
Abstract
We conducted this study to investigate antioxidant and cytotoxicity of ethanol extracts of Leonurus sibiricus L. Also, we examined the cytoprotected effect of ethanol extracts of Leonurus sibiricus L. about antihemolytic of Sprague Dawley rat red blood cell. After extracting Leonurus sibiricus L. with ethanol. We treated samples by concentration and conducted DPPH radical scavenging activity and SOD-like activity. About human skin fibroblast HS68 cell, through MTT assay, we assessed toxicity of samples. Also, to assess effect of antihemolytic, we observed effect of extracts which we got after inducing oxidation of Sprague Dawley rat red blood cell using H2O2. The result showed that extracts of Leonurus sibiricus L. was effective as concentration of DPPH radical scavenging activity and SOD-like activity increased. After we checked cytotoxicity of Leonurus sibiricus L. using human fibroblast HS68 cell, it showed life rate more than 90 percent at 5~100 μg/ml and we made sure of its safety for skin. In the experiment for assessing of anti-hemolytic, we could observe the group that treated with extracts showed the higher rate of anti-hemolytic than control group that treated with H2O2 only. This result showed by eliminating ROS, extracts of Leonurus sibiricus L. could protect cell membrane and work as antioxidants. These results were that extracts of Leonurus sibiricus L. can eliminate active oxygen specie, have no cytotoxicity in fibroblast cell and show actual effectiveness of cell protection about anti-hemolytic activity in red blood cell, so they show possibility as functional natural product.
참고문헌 (40)
- 김기호, "웰빙산업에서의 천연 물 연구 동향", 대한화장품학회지, 30, pp.329~343 (2004)
- 김미혜, "다류소재 식물류 중의 항산화물질 함량 분석", 한국식품과학회지, 31, pp.273~279 (1999)
- 김선제, "益母草와 茺蔚子의 高脂血症 억제 효능", 대한본초학회지, 25(3), pp.73~80 (2010), 대한본초학회
- 김영훈, "천연복합물(상황버섯, 감초, 병풀)의 항산화 및 세포독성에 관한 연구", 한국미용학회지, 17(3), pp.441~446 (2011), 한국미용학회
- 김인덕, "한방원료의 초임계 추출을 이용한 항노화 및 주름개선 효과", KSBB Journal, 23(6), pp.529~534 (2008), 한국생물공학회
- 김지영, "익모초 추출물의 항산화 작용에 관한 연구", 대한화장품학회지, 31, pp.189~196 (2005)
- 김학순, "익모초 추출액이 간장해에 미치는 영향", 한국위생과학회지, 5, pp.93~100 (1999)
- 박수남, "O2으로 유도된 사람 적혈구의 광용혈에 있어서 대두의 아이소플라본인 제니스테인의 보호작용", 한국식품과학회지, 35(3), pp.510~518 (2003), 한국식품과학회
- 배윤호, "속단, 익모초 및 향부자의 독성평가를 위한 성분분석 및 안정성 시험", 생약학회지, 43(1), pp.79~84 (2012), 한국생약학회
- 석호문, "도정부산물로부터 분리한 보리 폴리페놀 추출물의 항상화 효과", 한국식품과학회지, 34(5), pp.889~892 (2002), 한국식품과학회
- 신순희, "익모초 의 약효성분에 관한 연구", 생약학회지, 15, pp.104~107 (1984)
- 이숙영, "동백나무 잎과 꽃 추출물의 항미생물 활성 및 항산화 효과", 한국약용작물학회지, 13(3), pp.93~100 (2005), 한국약용작물학회
- 이희경, "엉겅퀴 추출물의 항상화성, 항돌연변이원성 및 항암활성 효과", 한국약용작물학회지, 11(1), pp.58~61 (2003), 한국약용작물학회
- 장태수, "복분자 에탄올 추출물의 항산화 및 항용혈 효능", 오일 및 응용과학 학회지, 31(1), pp.130~135 (2014), 한국유화학회[DOI]
- 조연경, "익모초추출물을 이용한 주름개선 기능성화장품 원료 개발", 석사, 향장미용학, pp.2011
- 차배천, "오배자의 항산화 활성성분 및자유라디컬 소거효과", 생약학회지, 121, pp.185~189 (2000)
- 홍성수, "익모초로부터 Leonurine의 분리 및 함량분석", 생약학회지, 32, pp.322~326 (2001)
- Agarwal R, "Involvement of singlet oxygen in chloroaluminum phthalocyanine tetrasulfonate - mediated photoenhancement of lipid peroxidation in rat epidermal microsomes", J. Cancer Letters, 56, pp.125~129 (1990)
- Aoshima H, "Aging of whiskey increases1,1-diphenyl-2-picrylhydrazl radical scavenging activity", J. Agric. Food Chem, 52, pp.5240~5244 (2004)
- Cai XH, "A new labdane diterpene from Leonurus heterophyllus", J. Asian Nat. Prod. Res, 8, pp.599~603 (2006)
- Devasagayam TP, "Free radicals and antioxidants in human health: current status and future prospects", J. Assoc. Physicians India, 52, pp.794~804 (2004)
- Edgington SM., "As we live and breathe: free radicals and aging. Correlative evidence from a number of fields suggests they may be key", J. BioTec, 12, pp.37~40 (1994)
- Foote CS., "Photosensitized oxygenations and the role of singlet oxygen", Acc. Chem. Res, 1, pp.104~110 (1968)
- Grasbon T, "Cu Zn superoxide dismutase transgenic retinal transplants", Graefes Arch. Clin. Exp. Ophthalmol, 237, pp.336~341 (1999)
- Giang PM, "Matsunami K Otsuka H. New bisspirolabdane-typediterpenoids from Leonurus heterophyllus", Chem. Pharm. Bull, 53, pp.1475~1479 (2005)
- Harman D., "A theory based on free radical and radiation chemistry", J. Gerontol, 11, pp.298~307 (1956)
- Hatano T., "Constituents of natural medicines with scavenging effects on active oxygen species-tannins and related polyphenols", Nat. Med, 49, pp.357~363 (1995)
- Kishi Y, "Synthesis of Leonurine", Tetrahedron Lett, 9, pp.637~640 (1968)
- Kitahara K, "A remarkable antioxidant effect of natural phenol derivatives on the antioxidation of γ-irradiated methyl linoleate", Chem. Pharm. Bull, 40, pp.2208~2209 (1992)
- Li Y, "Hepatoprotective glycosides from Leonurus japonicus Houtt", Carbohydr. Res, 348, pp.42~44 (2012)
- Liang H, "Protective effects of alkaloid extract from Leonurus heterophyllus on cerebral ischemia reperfusion injury by middle cerebral ischemic injury (MCAO) in rats", Phytomedicine, 18, pp.811~818 (2011)
- Magdalena LC, "Reactive oxygen species, cellular redox system, and apoptosis", Free Radic. Biol. Med, 48, pp.749~762 (2010)
- Mckersie BD, "Iron superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidativestress tolerance", Plant Physiol, 122, pp.1427~1437 (2000)
- Mnonopi N, "The cardioprotective effects of marrubiin, a diterpenoid found in Leonotis leonurus extracts", J. Ethnopharmacol, 138, pp.67~75 (2011)
- Rice-Evans C, "Sickle cell membranes and oxidative damage", J. Biochem, 237, pp.265~269 (1986)
- Sadrzadeh SM, "Hallaway PE, Eaton JW. Hemoglobin. biologic fenton reagent", J. Biol. Chem, 259, pp.143~154 (1984)
- Sikander M, "Cytoprotective activity of a transchalcone against hydrogen peroxide induced toxity in hepatocellular carcinoma(HepG2) cells", Asian Pac. J. Cancer Prev, 12, pp.2513~2516 (2011)
- Sugiura S, "Structure and synthesis of leonurine", Tetrahedron Lett, 25, pp.5155~5161 (1969)
- Valko M, "Free radicals and antioxidants in normal physiological functions and human disease", Int. J. Biochem. Cell Biol, 39, pp.44~84 (2007)
- Vives Corrons JL, "Increased susceptibility of microcytic red blood cells to in vitro oxidative stress", Eur. J .Haematol, 55, pp.327~331 (1995)