황금(黃芩) 추출물의 항산화 활성 및 세포독성 연구
A Study on the Antioxidant Activity and Cytotoxicity of Scutellaria baicalensis Extracts
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This research, in an attempt to determine the antioxidant activity of Scutellaria baicalensis extract, extracted 99% ethane with post-extraction retention measured at 2.05%. In addition the total polyphenol content in 1 ㎎/㎖ was analyzed to be 61.58±0.07 ㎎(GAE)/g and flavonoid content was found to be 357.17±5.48 ㎎(TAE)/g. The result of ABTS radical elimination ability measurement showed Vit C measuring at 95.8±0.80%. In extraction from Scutellaria baicalensis, the ability of Scutellaria baicalensis extract ABTS to eliminate at 10, 50, 100, 500, 1000 ㎍/㎖ concentration each showed 10.11±0.25%, 24.89±0.44%, 41.80±1.32%, 90.94±0.10%, 95.19±0.059% concentration, an interdependent increase. In measuring the results of the elimination ability of the DPPH radical, Vit C had an elimination ability of 94.9±0.08% and the gold extract for each concentration with the following increase 10.84±2.10%, 57.29±0.83%, 86.81±0.80%, 97.35±0.10% 97.35±0.14% antioxidant activity also increased confirming that the Scutellaria baicalensis extract possesses similar antioxidant capacity. In low concentration, the cell survival rate result was high, confirming cytotoxicity to be low. Based on the results of this research, the contents can be utilized as vital basic data in the development of natural functional skin-care product materials utilizing Scutellaria baicalensis extracts.
Abstract
This research, in an attempt to determine the antioxidant activity of Scutellaria baicalensis extract, extracted 99% ethane with post-extraction retention measured at 2.05%. In addition the total polyphenol content in 1 ㎎/㎖ was analyzed to be 61.58±0.07 ㎎(GAE)/g and flavonoid content was found to be 357.17±5.48 ㎎(TAE)/g. The result of ABTS radical elimination ability measurement showed Vit C measuring at 95.8±0.80%. In extraction from Scutellaria baicalensis, the ability of Scutellaria baicalensis extract ABTS to eliminate at 10, 50, 100, 500, 1000 ㎍/㎖ concentration each showed 10.11±0.25%, 24.89±0.44%, 41.80±1.32%, 90.94±0.10%, 95.19±0.059% concentration, an interdependent increase. In measuring the results of the elimination ability of the DPPH radical, Vit C had an elimination ability of 94.9±0.08% and the gold extract for each concentration with the following increase 10.84±2.10%, 57.29±0.83%, 86.81±0.80%, 97.35±0.10% 97.35±0.14% antioxidant activity also increased confirming that the Scutellaria baicalensis extract possesses similar antioxidant capacity. In low concentration, the cell survival rate result was high, confirming cytotoxicity to be low. Based on the results of this research, the contents can be utilized as vital basic data in the development of natural functional skin-care product materials utilizing Scutellaria baicalensis extracts.
참고문헌 (42)
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