익모초 추출물의 항산화 효과와 자외선으로 유도된 각질형성세포 손상에 대한 보호효과
Antioxidant and Protective Effects of Leonurus sibiricus L. Extract on Ultraviolet B (UVB)-induced Damage in Human Keratinocytes
1
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
적 요본 연구에서는 익모초 추출물의 항산화 효과 및 자외선으로유도된 각질형성세포 손상에 대한 보호효과를 확인하고자 익모초 열수 추출물의 생체 내 산화적 스트레스와 관련되어 있는DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide radical, lipid peroxidation에 대한 익모초 열수추출물의 소거효과 및 환원력 평가 시험을 진행하였으며, 사람 각질형성세포주인 HaCaT 세포주에서 UVB로 유도된 apoptosis에 대한 익모초의 억제 정도를 확인하였다. 그 결과, 익모초 열수추출물에서농도 의존적으로 항산화 효과가 증가함을 확인할 수 있었으며, UVB로 유도된 apoptosis에 발현 또한 억제됨을 확인할 수 있었다. 현재 국내외적으로 천연물에 대한 항산화 활성에 대한 연구가 활발히 진행되고 있는데, 이를 바탕으로 하는 피부 보호 효과에 대한 연구는 항노화 측면에서 그 효용가치가 높다고 여겨진다. 따라서 본 실험 연구의 결과를 토대로 항산화 효과가 우수한식물종에 대해 단일 물질에 대한 분리 작업과 함께 항노화 실험뿐만 아니라 항염증이나 항암 등의 실험이 더 깊이 있게 진행된다면 새로운 천연물 유래 생리 활성 물질로서 효과적이면서도안전한 화장품 소재로의 활용 또한 가능할 것으로 기대된다
Abstract
In this study, we investigated the anti oxidative potential and protective effects of water extract of Leonurus sibiricus L. leaf (LSLW) against ultraviolet B (UVB)-induced oxidative damage in human keratinocytes (HaCaT cells). To evaluate the anti oxidative activity of LSLW, we measured DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion scavenging activities, lipid peroxidation inhibition, and reducing power of LSLW. For induction of oxidative stress in HaCaT cells, the cells were irradiated with UVB at 40 mJ/㎠. To investigate the protective effects of LSLW against UVB, we measured cell viability and apoptotic bodies using annexin V staining. LSLW showed anti oxidative activities by scavenging DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion and by reducing lipid peroxidation. In addition, LSLW showed high reducing values. The UVB-induced oxidative conditions led to cell apoptosis. However, treatment with LSLW ameliorated oxidative stress conditions, including inhibition of cell death, apoptotic body. Taken together, LSLW exhibited anti oxidative and protective effects against UVB-induced damage in HaCaT cells. Thus, LSLW could be useful for the development of cosmetics for UVB-induced skin aging. Key words - Leonurus sibiricus L. leaf, Ultraviolet B, Human keratinocytes, Anti oxidative activity, Apoptosis
참고문헌 (39)
- Afaq, F., "Photochemoprevention of ultraviolet B signaling and photocarcinogenesis", Mutat Res, 57, pp.1153~1173 (2005)
- Boalino, D. M., "Labdane diterpenes of Leonurus sibiricus", J. Nat. Prod, 67, pp.714~717 (2004)
- 부희옥, "Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants", 한국자원식물학회지, 25(3), pp.349~356 (2012), 한국자원식물학회[DOI]
- Brand-Williams W., "Use of a free radical method to evaluate antioxidant activity", Food Sci. Technol-Leb, 28(1), pp.25~30 (1995)
- Chao, Z., "Determination of stachydrine and leonurine in Herba Leonuri by ion-pair reversed-phase high-performance liquid chromatography", Academic Journal of the First Medical College of PLA, 24, pp.1223~1226 (2004)
- 조원준, "금은화 추출물의 항산화 효과를 통한 세포 보호효과 및 유전자 발현 양상", 생명과학회지, 23(8), pp.989~997 (2013), 한국생명과학회[DOI]
- Choi, C. W., "Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison", Plant Sci, 163(6), pp.1161~1158 (2002)
- 최근표, "약용식물의 Angiotensin converting enzyme 저해활성 탐색", 한국약용작물학회지, 10(5), pp.399~402 (2002), 한국약용작물학회
- Chung, S. K., "Hydroxyl radical-scavanging effects of spices and scavengers from brown mustard(Brassica nigra)", Biosci. Biotech. Biochem, 61(1), pp.118~123 (1997)
- Adriana Filip, "Photoprotective Effects of Two Natural Products on Ultraviolet B–Induced Oxidative Stress and Apoptosis in SKH-1 Mouse Skin", Journal of Medicinal Food, 14(7), pp.761~766 (2011), 한국식품영양과학회
- Fuchs, J., "Oxidative Stress: Oxidants and Antioxidants", pp.559~583 (1991), Academic Press
- Ghee, S. S., "Effects of Chrthami flos and Leonuri herba on the liver damaged with carbon tetrachloride treatment in rats", Korean J. Herbology, 16, pp.79~89 (2001)
- Godar, D. E., "Preprogrammed and programmed cell death mechanisms of apoptosis : UV-induced immediate and delayed apoptosis", Photochem Photobiol, 63, pp.825~830 (1996)
- Halliwell, B., "Oxidants and human disease : some new concepts", Fase. J, 1, pp.258~364 (1987)
- Heck, D. E., "Solar ultraviolet radiation as a trigger of cell signal transduction", Toxicol. Appl. Pharmacol, 195, pp.288~297 (2004)
- Hong, S. S., "Isolation and quantitative analysis of leonurine from Leonuri herba", Korean J. Pharmacogn, 32, pp.322~326 (2001)
- 서지형, "추출조건에 따른 익모초 물추출물의 생리활성 비교", 한국식품저장유통학회지, 20(4), pp.592~596 (2013), 한국식품저장유통학회
- 김선제, "益母草와 茺蔚子의 高脂血症 억제 효능", 대한본초학회지, 25(3), pp.73~80 (2010), 대한본초학회
- Kulms, D., "Nuclear and cell membrane effects contribute independently to the induction of apoptosis in human cells exposed to UVB radiation", Proc. Natl. Acad. Sci, 96(14), pp.7974~7979 (1999)
- Lee, S. W., "Compounds and anti-oxidative activities from the aerial parts of Leonuri herba.", Chung-Ang J. Pharm. Sci, 20, pp.53~59 (2006)
- Liu, F., "Free radical scavenging activities of mushroom olysaccharide extract", Life Sci, 60, pp.763~771 (1997)
- Malorni, W., "Both UVA and UVB induce cytoskeleton-dependent surface blebbing in epidermoid cells", Journal of Photochemistry and Photobiology B : Biology, 26(3), pp.265~270 (1994)
- Moll, I., "Effects of ultraviolet B radiation on cytoskeletal and adhesion molecules in human epidermis", Photodermatol Photoimmunol Photomed, 10, pp.26~32 (1994)
- Nagasawa, H., "Further study on the effects of motherwort(Leonurus sibiricus L. )on preneoplastic and neoplastic mammary gland growth in multiparous GR", A mice, 1992, pp.12, 141~143
- Oyaizu, M., "Studies on products of browning reaction : antioxidative activities of products of browning reaction prepared from glucosamine", Jpn. J. Nutr, 44, pp.307~315 (1986)
- Park, K. K., "Antihypertensive effects of Pini folium and Leonuri herba extract on spontaneously hypertensive rat(SHR)", J. Applied Pharmacology, 8, pp.27~31 (2000)
- Pontin, M. A., "Transcriptome changes in grapevine(Vitis vinifera L. cv. Malbec)leaves induced by ultraviolet-B radiation", BMC Plant Biol, 10, pp.224~ (2010)
- Professors of Herbology in College of Korean Medicine., "Textbook of Herbology", pp.127~583 (2002), Younglimsa Publishing Co
- Pugliese, P. T., "The skin, free radicals, and oxidative stress", Dermatol. Nurs, 7, pp.361~369 (1995)
- Satoh, M., "Studies on the constituents of Leonurus sibiricus L", Chem. Pharm. Bull, 51, pp.341~342 (2003)
- Shin, S. H., "Studies on active principles of Leonurus sibiricus", Korean J. Pharmacogn, 15, pp.104~107 (1984)
- Shindo, Y., "Ultraviolet B-induced cell death in four cutaneous cell lines exhibiting different enzymatic antioxidant defences : involvement of apoptosis", J Dermatol Sci, 17, pp.140~150 (1998)
- Shlafer, M., "A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances", Analytical Biochemistry, 137(2), pp.269~276 (1984)
- Verschooten, L., "Adaptive response of the skin to UVB damage : role of the p53 protein", Int. J. Cosmetic Sci, 28, pp.1~7 (2006)
- Yaar, M., "Photoageing : mechanism, prevention and therapy", British Journal of Dermatology, 157(5), pp.874~887 (2007)
- 양윤정, "국내 자생 자원식물들의 항산화 활성 검색", 한국자원식물학회지, 24(1), pp.1~9 (2011), 한국자원식물학회
- Zamansky, G. B., "Environmental wavelengths of ultraviolet light induce cytoskeletal damage", J. Invest. Dermatol, 89, pp.603~606 (1987)
- Zamansky, G. B., "Disruption of keratin intermediate filaments by ultraviolet radiation in cultured human keratinocytes", Photochem. Photobiol, 52, pp.903~906 (1990)
- Zamansky, G. B., "Disruption of cytoplasmic microtubules by ultraviolet radiation", Exp. Cell. Res, 195, pp.269~273 (1991)