황칠나무 잎(Dendropanax morbifera. Leaf) 에탄올 추출물의 화장품 소재로서 생리활성 검증
Verification of Physiological Activities of Dendropanax morbiferus Leaf Ethanol Extract as Cosmetic Ingredient
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
This study attempted to test the possibility of Dendropanax morbiferus leaf extract as a cosmetic ingredient. The extracted was combined with ethanol and amount of polyphenol, flavonoid, DPPH radical scavenging activity, nitric oxide(NO) scavenging activity, superoxide dismutase(SOD)-like activity, cytotoxicity, and antimicrobial activity were measured, and the results found the followings: The total polyphenol and flavonoid contents were 48.77±1.52 mg/g and 31.86±1.44 mg/g respectively. The DPPH radical scavenging activity increased as the Dendropanax morbiferus leaf and ethanol extract became more concentrated. The NO scavenging activity inhibited NO generation according to the concentration and was highly dose-dependent according to the measurement of the SOD-like activity. In terms of cytotoxicity, survival rates decreased as the extract concentration increased. However, they rose at 0.1 and 0.5 mg/mL, confirming cell-protection effects. According to antimicrobial activity, a clear zone was not found on 1 mg/disc of S. aureus and S. epidermidis. However, such a zone was observed on 5 mg/disc with higher concentrations, confirming antimicrobial effects. The above results confirm that Dendropanax morbiferus leaf extract with ethanol has antioxidant, anti-inflammatory, and antimicrobial effects. Therefore, it is reasonable to conclude that the extract from the leaves of Dendropanax morbiferus has great potential as an ingredient for personal care cosmetics, such as skin and hair care products.
Abstract
This study attempted to test the possibility of Dendropanax morbiferus leaf extract as a cosmetic ingredient. The extracted was combined with ethanol and amount of polyphenol, flavonoid, DPPH radical scavenging activity, nitric oxide(NO) scavenging activity, superoxide dismutase(SOD)-like activity, cytotoxicity, and antimicrobial activity were measured, and the results found the followings: The total polyphenol and flavonoid contents were 48.77±1.52 mg/g and 31.86±1.44 mg/g respectively. The DPPH radical scavenging activity increased as the Dendropanax morbiferus leaf and ethanol extract became more concentrated. The NO scavenging activity inhibited NO generation according to the concentration and was highly dose-dependent according to the measurement of the SOD-like activity. In terms of cytotoxicity, survival rates decreased as the extract concentration increased. However, they rose at 0.1 and 0.5 mg/mL, confirming cell-protection effects. According to antimicrobial activity, a clear zone was not found on 1 mg/disc of S. aureus and S. epidermidis. However, such a zone was observed on 5 mg/disc with higher concentrations, confirming antimicrobial effects. The above results confirm that Dendropanax morbiferus leaf extract with ethanol has antioxidant, anti-inflammatory, and antimicrobial effects. Therefore, it is reasonable to conclude that the extract from the leaves of Dendropanax morbiferus has great potential as an ingredient for personal care cosmetics, such as skin and hair care products.
참고문헌 (38)
- 화장품 중유해물질 분석법 연구, "최보경", 의료제품연구부 화장품연구팀, 식품의약품안전청, ~ (2011)
- "화학첨가물 NO... 노케미족·노푸족늘어나" (2017)[URL]
- 배준태, "생물전환기술을 이용한 감초추출물의 주름개선 화장품 소재 개발", 박사, pp.2020
- 임정묵, "화장품 소재 개발을 위한 대두 추출물의 생리활성 평가", 대한미용학회지, 16(1), pp.11~22 (2020), 대한미용학회[DOI]
- 이소현, "건강기능성 식품 및 화장품 소재개발을 위한 어수리 잎 추출물의 기능성 탐색", 석사, pp.2018
- 전경미, "녹나무와 동백 씨 추출물 유래기능성화장품 소재개발", 박사, pp.2016
- 정갑섭, "소리쟁이 에탄올 추출물의 항산화 활성 및 뿌리의 중금속 흡착능에 관한 연구", 한국산학기술학회논문지, 13(2), pp.934~940 (2012), 한국산학기술학회
- 한종수, "황칠(黃漆)", 동방논집, 3(1), pp.1~14 (2010)
- 이시진, "본초강목, 3권", pp.150~155, 중국문광도서
- 김형량, "황칠나무 잎 및 종실의화학적 특성", 한국농화학회지, 43(1), pp.63~66 (2000)
- 박수아, "황칠나무 잎 추출물의 세포 항산화 활성과 미백활성 측정", 한국미생물·생명공학회지, 41(4), pp.407~415 (2013), 한국미생물·생명공학회[DOI]
- 김종민, "High Glucose로 유도된 산화 스트레스에 대한 황칠나무 잎 추출물의 뇌신경세포 보호 효과", 한국식품영양과학회지, 45(7), pp.938~947 (2016), 한국식품영양과학회
- 이서호, "황칠나무 잎의 면역활성증진기능 탐색", 한국약용작물학회지, 10(2), pp.109~115 (2002), 한국약용작물학회
- M. A. Amerine, "Method for analysis of musts and wine", pp.176~180 (1980), Wiley & Sons
- M. I. Moreno, "Comparison of the free radical-scavenging activity of propolis from several region of Argentina", Journal of Enthropharmacology, 71(1-2), pp.109~123 (2000)
- M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature, 181(4617), pp.1199~1200 (1958)
- S. Marklund, "Involvement of the superoxide anion radical in the au toxidation of pyrogallol and a convenient assay for superoxide dismutase", European Journal Biochemistry, 47(3), pp.469~543 (1974)
- L, Marcocci, "The nitric oxidescavenging properties of Ginkgo biloba extract EGb 761", Biochemical and Biophysical Research Communications, 201(2), pp.748~755 (1994)
- 신경민, "Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과", 생약학회지, 34(3), pp.223~227 (2003), 한국생약학회
- C. H. Collins, "Collins and Lyne's microbiological methods", pp.178~205 (1995), Butterworth-Heinemann Ltd
- 김소은, "화장품 소재로서 세발나물 추출물의 생리활성 연구", 한국인체미용예술학회지, 20(2), pp.59~71 (2019), 한국인체미용예술학회[DOI]
- 허수현, "아카시아 잎 추출물의 생리 활성 및 고분자 미셀과 세포투과 펩티드를 적용한 피부흡수증진 효과", 문화기술의 융합, 5(3), pp.271~282 (2019), 국제문화기술진흥원
- 김인혜, "모링가 잎 (Moringa oleifera, Leaf) 에탄올 추출물의 화장품 소재 특성", KSBB Journal, 33(1), pp.34~40 (2018), 한국생물공학회[DOI]
- 김경철, "굴거리 열매 추출물의 폴리페놀 함량과 항산화 활성", 공학기술논문지, 12(4), pp.149~155 (2019), 공학기술연구원[DOI]
- 장지연, "아사이베리 에탄올 추출물과 열수 추출물의 생리활성 검증", 한국인체미용예술학회지, 16(1), pp.97~109 (2015), 한국인체미용예술학회
- 황석준, "돌미나리 추출물의 함유성분 분석과 항산화 활성", 한국식품과학회지, 45(2), pp.227~234 (2013), 한국식품과학회[DOI]
- 신희준, "참죽나무 잎 추출물의 생리활성", KSBB Journal, 23(2), pp.164~168 (2008), 한국생물공학회
- 곽희진, "양파 메탄올 추출물의 생리활성 및 항산화효과", 한국식품영양과학회지, 29(2), pp.349~355 (2000)
- D. M. Bryan, "Iron-superoxide dismutase expression in transgenic alfalfa increase winter survival without a detectable increase in photosynthetic oxidative stress tolerance", Plant Physiology, 122(4), pp.1427~1437 (2000)
- 모정희, "노근 에탄올과 열수 추출물의 항산화 활성비교", 한국미용학회지, 19(5), pp.809~814 (2013), 한국미용학회
- 이양숙, "금은화, 백선피, 연교 추출물의 항노화, 항산화 및 항염증 활성", 대한미용학회지, 16(1), pp.1~10 (2020), 대한미용학회[DOI]
- 장지연, "퍼머넌트 웨이브 모발에 황칠나무 잎 추출물 트리트먼트 후 자외선 조사에따른 모발 보호 효과", 박사, pp.2015
- 김영훈, "신이화(辛夷花, Magnoliae Flos)추출물의 화장품약리활성 및 항염증효과에 관한 연구", 생명과학회지, 22(6), pp.730~735 (2012), 한국생명과학회[DOI]
- H. T. Chung, "Nitric oxide as a bioregulator of apoptosis", Biochemical and Biophysical Research Communications, 282(5), pp.1075~1084 (2001)
- 유단희, "패션프루트 껍질의 생리활성에 대한 연구", 한국인체미용예술학회지, 21(2), pp.143~154 (2020), 한국인체미용예술학회[DOI]
- 이경인, "비파 잎 추출물의 항산화 및 항균활성", 한국식품영양과학회지, 38(3), pp.267~273 (2009), 한국식품영양과학회
- 이수경, "황칠나무 잎 에탄올 추출물의항균 및 항산화활성", 석사, pp.2017
- 손호용, "피부 여드름 치료제 개발을 위한 천연물의 항균활성 검색", 한국미생물·생명공학회지, 34(3), pp.265~272 (2006), 한국미생물·생명공학회