상백피 추출물의 생물전환과 티로시나제 저해 효과의 비교
Inhibition of Tyrosinase by Bio-conversioned Morus alba Extract
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Skin lightening is one of the most important effect of functional cosmetics, especially for the asian women. For this reason, many kinds of inhibitors of melanin synthesis by melanocyte have been developed since a decade ago. Although many kinds of melanogenesis inhibitors such as arbutin, ascorbic acid, extract of Licorice root or Morus alba and niacinamide were recommended as the standard component of the skin whitening cosmetics by Korean Food and Drug Administration, they are not always satisfactory to the many users. Mulberroside A, a main component of extract of Morus alba balk, can be converted into an aglycan named oxyresveratrol having a strong antioxidant and anti-melanogenesis effect using β-glucosidase. However, the glucose hydrolysis process using a soluable β-glucosidase is not suitable to convert Mulberrosie A into oxyresveratrol in a large scale procss because of the low reaction rate and high price. In this work, we developed a bioconversion process of oxyresveratrol production from Morus alba extract using enzyme named Accellerase®, which is an immobilized enzyme on the solid particle, and compared the tyrosinase inhibition effect of the extracts of before and after the bioconversion process. It was found that the inhibition effect of Morus alba extract to the mushroom tyrosinase was greatly increased by the bioconversion process and this effect was synergized when the extract was used with arbutin together.
Abstract
Skin lightening is one of the most important effect of functional cosmetics, especially for the asian women. For this reason, many kinds of inhibitors of melanin synthesis by melanocyte have been developed since a decade ago. Although many kinds of melanogenesis inhibitors such as arbutin, ascorbic acid, extract of Licorice root or Morus alba and niacinamide were recommended as the standard component of the skin whitening cosmetics by Korean Food and Drug Administration, they are not always satisfactory to the many users. Mulberroside A, a main component of extract of Morus alba balk, can be converted into an aglycan named oxyresveratrol having a strong antioxidant and anti-melanogenesis effect using β-glucosidase. However, the glucose hydrolysis process using a soluable β-glucosidase is not suitable to convert Mulberrosie A into oxyresveratrol in a large scale procss because of the low reaction rate and high price. In this work, we developed a bioconversion process of oxyresveratrol production from Morus alba extract using enzyme named Accellerase®, which is an immobilized enzyme on the solid particle, and compared the tyrosinase inhibition effect of the extracts of before and after the bioconversion process. It was found that the inhibition effect of Morus alba extract to the mushroom tyrosinase was greatly increased by the bioconversion process and this effect was synergized when the extract was used with arbutin together.
참고문헌 (20)
- 강정란, "민들레분획물이 미백활성에 미치는 효과에 대한 연구", 대한피부미용학회지, 7(4), pp.199~206 (2009), 대한피부미용학회
- 김동현, "스피루리나 발효에 의한 항산화력 증진 및 항노화 효과", 석사, 화학공학, pp.2009
- 김미진, "상백피 추출물로부터 생물전환에 의한 Oxyresveratrol의 생산", 석사, 생명화공, pp.2011
- 김현수, "유산균의 항산화 효과", 한국축산식품학회지, 23(2), pp.186~192 (2003), 한국축산식품학회
- 박대중, "三白草 에탄올추출물의 항산화능 및 피부 미백작용 연구", 대한본초학회지, 23(2), pp.193~202 (2008), 대한본초학회
- 박서영, "Whitening Effects of Mori Ramulus, Mori Cortex Radicis and Mori Folium Herbal-acupuncture Solution after Fermentation and Heating", 대한침구의학회지, 26(1), pp.91~98 (2009), 대한침구의학회
- 박수남, "식물유래 성분의 미백화장품(기능성화장품)에의 응용", 논문집, 52, pp.205~220 (2001)
- 박효정, "효소처리한 홍삼의 기능성화장품 적용에 대한 연구", 박사, 식품영양, pp.2010
- 배유경, "오매 추출물의 항산화 활성 및 화장품소재 가능성에 관한 연구", 박사, 미생물공학과, pp.2012
- 양서진, "연교 추출물의 항산화 활성 및 화장품 소재로서의 연구", 박사, 미생물공학과, pp.2012
- 오수정, "함초와 발효 함초 추출물의 화장품 소재로서 생리활성 비교연구", 대한피부미용학회지, 9(4), pp.1~8 (2011), 대한피부미용학회
- 유민정, "SK-MEL-2에서 상백피 추출물의 Tyrosinase 활성 억제 및 Melanin 생성 억제 효과", 대한피부미용학회지, 9(4), pp.1~12 (2011), 대한피부미용학회
- 최은영, "발효 상백피 추출물이 첨가된 화장품이 여성 피부에 미치는 영향", 박사, 미생물공학, pp.2011
- 한영숙, "천연식물추출물의 항산화력과 미백작용의 상관관계연구", 대한피부미용학회지, 1(1), pp.11~22 (2003), 대한피부미용학회
- 한정선, "솔잎 발효액의 항산화 활성 및 멜라닌 생성 억제 효과", 대한피부미용학회지, 10(3), pp.619~624 (2012), 대한피부미용학회
- Curto EV, "Inhibitors of mammalian melanocytes tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors", Biochem. Pharmacol, 57, pp.663~672 (1999)
- Grove GL, "Skin replica analysis of photodamaged skin after therapy with tretinoin emollient cream", J. Am. Acad. Dermatol, 25, pp.231~237 (1991)
- Lee CH, "investigation of melanin biosynthesis inhibition substance", Kor Soc. Appl. Microbiol. Biotechnol, 9, pp.32~32 (1996)
- Maeda K, "In vivo effectiveness of several whitening cosmetic components in human melanocytes", J. Soc. Cosmet. Chem, 56, pp.397~401 (2001)
- Pawelek JM, "The biosynthesis of mammalian melanin", Am. Sci, 70, pp.91~126 (1982)