제니스테인의 멜라닌 생성 억제 및 In vivo 미백 효과
The Effect of Genistein on Melanin Synthesis and In vivo Whitening.
2
조회
0
다운로드
26
인용
0
참고문헌
초록
The effect of genistein on melanin synthesis was studied using in vitro and in vivo model. Genistein inhibited melanin synthesis in cultured melan-a cells dose dependently. Tyrosinase activity was decreased by genistein treatment in melan-a cells, but genistein did not inhibit tyrosinase directly. Genistein did not affect the expression of tyrosinase in melan-a cells. Genistein inhibited the activity of α-glucosidase in vitro and the glycosylation of tyrosinase in melan-a cells. The resulting unsaturated glycosylation of tyrosinase makes it unstable and disturb correct transportation. To further clarify the effect of genistein on the melanogenesis, we established UVBinduced hyperpigmentation on the shaved backs of brown guinea pigs. The animals were exposed to UVB radiation once a week for three consecutive weeks. Genistein (1 and 2%) or vehicle alone as a control were then topically applied to the hyperpigmented areas daily. Genistein showed significant lightening effect on the UVB-induced hyperpigmentation in five weeks. Depigmenting effect was prominent in 2% genistein treatment with Fontana-Masson staining. In conclusion, genistein may be a useful agent for skin whitening.
Abstract
The effect of genistein on melanin synthesis was studied using in vitro and in vivo model. Genistein inhibited melanin synthesis in cultured melan-a cells dose dependently. Tyrosinase activity was decreased by genistein treatment in melan-a cells, but genistein did not inhibit tyrosinase directly. Genistein did not affect the expression of tyrosinase in melan-a cells. Genistein inhibited the activity of α-glucosidase in vitro and the glycosylation of tyrosinase in melan-a cells. The resulting unsaturated glycosylation of tyrosinase makes it unstable and disturb correct transportation. To further clarify the effect of genistein on the melanogenesis, we established UVBinduced hyperpigmentation on the shaved backs of brown guinea pigs. The animals were exposed to UVB radiation once a week for three consecutive weeks. Genistein (1 and 2%) or vehicle alone as a control were then topically applied to the hyperpigmented areas daily. Genistein showed significant lightening effect on the UVB-induced hyperpigmentation in five weeks. Depigmenting effect was prominent in 2% genistein treatment with Fontana-Masson staining. In conclusion, genistein may be a useful agent for skin whitening.
참고문헌 (35)
- Afaq, F, "Botanical antioxidants in the prevention of photocarcinogenesis and photoaging", Exp. Dermatol, 15, pp.678~684 (2006)
- Alaluf, S., "Variation in melanin content and composition in type V and VI photoexposed and photoprotected human skin: the dominant role of DHI", Pigment Cell Res, 14, pp.337~347 (2001)
- Ando, H., "Linoleic and α-linoleic acid lightens ultravioletinduced hyperpigmentation of the skin", Arch. Dermatol. Res, 290, pp.375~381 (1998)
- Angelov, A., "Molecular and biochemical characterization of alpha-glucosidase and alpha-mannosidase and their clustered genes from the thermoacidophilic archaeon Picrophilus torridus", J. Bacteriol, 188, pp.7123~7131 (2006)
- Battaini, G., "Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid", J. Biol. Inorg. Chem, 5, pp.262~268 (2000)
- Bennett, D. C., "A line of non-tumorigenic mouse melanocytes, syngeneic with the B16 melanoma and requiring a tumour promoter for growth", Int. J. Cancer, 39, pp.414~418 (1987)
- Bolognia, J. L., "Hairless pigmented guinea pigs: a new model for the study of mammalian pigmentation", Pigment Cell Res, 3, pp.150~156 (1990)
- Branza-Nichita, N., "Tyrosinase folding and copper loading in vivo: a crucial role for calnexin and alpha-glucosidase II", Biochem. Biophys. Res. Commun, 261, pp.720~725 (1999)
- Branza-Nichita, N., "Mutations at critical N-glycosylation sites reduce tyrosinase activity by altering folding and quality control", J. Biol. Chem, 275, pp.8169~8175 (2000)
- Briganti, S., "Chemical and Instrumental Approaches to Treat Hyperpigmentation", Pigment Cell Res, 16, pp.101~110 (2003)
- Chang, T. S., "Identifying 6,7,4'-trihydroxyisoflavone as a potent tyrosinase inhibitor", Biosci. Biotechnol. Biochem, 69, pp.1999~2001 (2005)
- Choi, H., "Deoxypodophyllotoxinreduces skin pigmentation of brown guinea pigs", Planta. Med, 70, pp.370~380 (2004)
- Choi, H., "Influence of N-glycan processing disruption on tyrosinase and melanin synthesis in HM3KO melanoma cells", Exp. Dermatol, 16, pp.110~117 (2007)
- Franchi, J, "Depigmenting effects of calcium D-pantetheine- S-sulfonate on human melanocytes", Pigment Cell Res, 13, pp.165~171 (2000)
- Fuller, B. B., "Alphamelanocyte- stimulating hormone regulation of tyrosinase in Cloudman S-91 mouse melanoma cell cultures", J. Biol. Chem, 262, pp.4024~4033 (1987)
- Goochee, C. F, "Bioprocess factors affecting glycoprotein oligosaccharide structure", Dev. Biol. Stand, 76, pp.95~104 (1992)
- Halaban, R. E., "Aberrant retention of tyrosinase in the endoplasmic reticulum mediates accelerated degradation of the enzyme and contributes to the dedifferentiated phenotype of amelanotic melanoma cells", Proc. Natl. Acad. Sci. USA, 94, pp.6210~6215 (1997)
- Hwang, J. S., "The inhibitory effects of 3,4,5-Trimethoxy cinnamate thymol ester(TCTE, Melasolv) on Melanogenesis", Journal of the Society of Cosmetic Scientists of Korea, 28, pp.135~149 (2002)
- Imokawa, G, "Functional analysis of tyrosinase isozymes of cultured malignant melanoma cells during the recovery period following interrupted melanogenesis induced by glycosylation inhibitors", J. Invest. Dermatol, 83, pp.196~201 (1984)
- Kim, D. S., "Ceramide inhibits cell proliferation trough Akt", PKB inactivation and decreases melanin synthesis in Mel-Ab Cells, 2001, pp.14, 110~115
- Kornfeld, R, "Assembly of asparaginelinked oligosaccharides", Annu. Rev. Biochem, 54, pp.631~664 (1985)
- Lee, D. S, "Genistein, a soy isoflavone, is a potent alpha-glucosidase inhibitor", FEBS Lett, 501, pp.84~86 (2001)
- Lee, K. H., "Antioxidantive properties of isoflavone, genistein from soybean and its application to whitening cosmetics" (2004), Seoul National University of Technology
- Maeda, K, "Arbutin: mechanism of its depigmenting action in human melanocyte culture", J. Pharmacol. Exp. Ther, 276, pp.765~769 (1996)
- Mishima, Y, "Selective aberration and pigment loss in melanosomes of malignant melanoma cells in vitro by glycosilation inhibitors: premelanosomes as glycoprotein", J. Invest. Dermatol, 81, pp.106~114 (1983)
- Petrescu, A. J., "The solution NMR structure of glucosylated N-glycans involved in the early stages of glycoprotein biosynthesis and folding", EMBO J, 16, pp.4302~4310 (1997)
- Petrescu, S. M., "Inhibition of N-glycan processing in B16 melanoma cells results in inactivation of tyrosinase but does not prevent its transport to the melanosome", J. Biol. Chem, 272, pp.15796~15803 (1997)
- Rauth, S., "Green. Inhibition of growth and induction of differentiation of metastatic melanoma cells in vitro by genistein: chemosensitivity is regulated by cellular p53", Br. J. Cancer, 75, pp.1559~1566 (1997)
- Wang, Y., "Synergetic inhibition of genistein and D-glucose on α- glucosidase", Bioorg. Med. Chem. Lett, 14, pp.2947~2950 (2004)
- Wang, Y., "Synergetic inhibition of metal ions and genistein on alpha-glucosidase", FEBS Lett, 576, pp.46~50 (2004)
- Wei, H., "Isoflavone genistein inhibits the initiation and promotion of two-stage skin carcinogenesis in mice", Carcinogenesis, 19, pp.1509~1514 (1998)
- Wei, H., "Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein", Nutr. Cancer, 20, pp.1~12 (1993)
- Wei, H., "Inhibition of ultraviolet light-induced oxidative events in the skin and internal organs of hairless mice by isoflavone genistein", Cancer Lett, 185, pp.21~29 (2002)
- Winchester, B, "Amino-sugar glycosidase inhibitors: versatile tools for glycobiologists", Glycobiology, 2, pp.199~210 (1992)
- Yan, C. H., "Effects of genistein, a soybean-derived isoflavone, on proliferation and differentiation of B16-BL6 mouse melanoma cells", J. Asian Nat. Prod. Res, 1, pp.285~299 (1999)
