밀몽화 추출물의 멜라닌 생합성 억제 효과 및 항산화 활성 효과
Effect of Buddleja officinalis MAXIM. on Melanin Biosynthesis and Anti-Oxidative Activity
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Melanin synthesis is related to the presence of tyrosinase. Therefore, tyrosinase inhibitors are useful skin-whitening agent. In this study Buddleia officinalis (B.O.) extract was used for searching the anti-oxidative and skin whitening effects. As a result, B.O. extracts showed over 50 % of free radical scavenging activity on DPPH at a concentration of 100 ㎍/㎖ compare to control. B.O. extracts showed 44 % inhibitory effect on tyrosinase activity in the mushroom tyrosinase of 100㎍/㎖. And EA fraction inhibited more than 70% of tyrosinase activity at the same concentration. Also, B.O. extracts showed effectiveness in inhibiting L-DOPA auto-oxidation. Melanin biosynthesis was estimated from B16 mouse melanoma cell, which treated with 10 nM of α-MSH and with or without B.O. extracts. B.O. extracts inhibited (> 40 %) melanin synthesis at 100㎍/㎖, but it did not affect on cell viability. This indicated B.O. extracts inhibited tyrosinase activity and melanin biosynthesis.
Abstract
Melanin synthesis is related to the presence of tyrosinase. Therefore, tyrosinase inhibitors are useful skin-whitening agent. In this study Buddleia officinalis (B.O.) extract was used for searching the anti-oxidative and skin whitening effects. As a result, B.O. extracts showed over 50 % of free radical scavenging activity on DPPH at a concentration of 100 ㎍/㎖ compare to control. B.O. extracts showed 44 % inhibitory effect on tyrosinase activity in the mushroom tyrosinase of 100㎍/㎖. And EA fraction inhibited more than 70% of tyrosinase activity at the same concentration. Also, B.O. extracts showed effectiveness in inhibiting L-DOPA auto-oxidation. Melanin biosynthesis was estimated from B16 mouse melanoma cell, which treated with 10 nM of α-MSH and with or without B.O. extracts. B.O. extracts inhibited (> 40 %) melanin synthesis at 100㎍/㎖, but it did not affect on cell viability. This indicated B.O. extracts inhibited tyrosinase activity and melanin biosynthesis.
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