In vitro and in vivo Biological Responses of Proton Irradiation from MC-50 Cyclotron
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, we investigated the biological damage and stress responses induced byion beam (proton beam) irradiation as a basis for the development of protective measures againstspace radiation. We examined the biological effects of proton beam produced by MC-50 cyclotronat KIRAMS on the cultured cells and mice. The proton beam energy used in this study was 34.9MeV and the absorption dose rate for cells and mice were 0.509 Gy sec-1 and 0.65 Gy sec-1, respectively. The cell survival rates measured by plating efficiency showed the different sensitivity anddose-relationship between CHO cells and Balb/3T3 cells. HGPRT gene mutation frequency in Balb/3T3 was 15×10-6 Gy-1, which was similar to the reported value of X-ray. When stress signalingproteins were examined in Balb/3T3 cells, IκB-α decreased markedly whereas p53, phospho-p53,and Rb increased after proton beam irradiation, which implied that the stress signaling pathwayswere activated by proton beam irradiation. In addition, cellular senescence was induced in IMR-90 cells. In the experiments with C57BL/6 mouse, the immune cells (white blood cells, lymphocytes)in the peripheral blood were greatly reduced following proton beam irradiation whereas red bloodcells and platelets showed relatively little change. These results can be utilized as basic data forstudying the biological effects of proton beam using MC-50 cyclotron with respect to proton therapyresearch as well as space radiation research.
Abstract
In this study, we investigated the biological damage and stress responses induced byion beam (proton beam) irradiation as a basis for the development of protective measures againstspace radiation. We examined the biological effects of proton beam produced by MC-50 cyclotronat KIRAMS on the cultured cells and mice. The proton beam energy used in this study was 34.9MeV and the absorption dose rate for cells and mice were 0.509 Gy sec-1 and 0.65 Gy sec-1, respectively. The cell survival rates measured by plating efficiency showed the different sensitivity anddose-relationship between CHO cells and Balb/3T3 cells. HGPRT gene mutation frequency in Balb/3T3 was 15×10-6 Gy-1, which was similar to the reported value of X-ray. When stress signalingproteins were examined in Balb/3T3 cells, IκB-α decreased markedly whereas p53, phospho-p53,and Rb increased after proton beam irradiation, which implied that the stress signaling pathwayswere activated by proton beam irradiation. In addition, cellular senescence was induced in IMR-90 cells. In the experiments with C57BL/6 mouse, the immune cells (white blood cells, lymphocytes)in the peripheral blood were greatly reduced following proton beam irradiation whereas red bloodcells and platelets showed relatively little change. These results can be utilized as basic data forstudying the biological effects of proton beam using MC-50 cyclotron with respect to proton therapyresearch as well as space radiation research.
참고문헌 (16)
- Belli M, "Molecular targets in cellular response to ionizing radiation and implications in space radiation protection", J. Radiat. Res, 43, pp.S13~S19 (2002)
- Cherubini R, "Minisatellite and Hprt mutations in V79 cells irradiated with helium ions and gamma rays", Int. J. Radiat. Biol, 78(9), pp.791~797 (2002)
- Das SK, "Fucoxanthin induces cell cycle arrest at G0", G1 phase in human colon carcinoma cells through up-regulation of p21WAF1, Biochim. Biophys. Acta, pp.2005, 1726(3)[DOI]
- Diamond AM, "The inhibition of radiation-induced mutagenesis by the combined effects of selenium and the aminothiol WR-1065", Mut. Res, 356, pp.147~154 (1996)
- Duli´c V, "p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induce d G1 arrest", Cell, 76, pp.1013~1023 (1994)
- Epelman S, "Medical mitigation strategies for acute radiation exposure during spaceflight", Aviat. Space Environ. Med, 77, pp.130~139 (2006)
- Hall EJ, "Radiobiology for the Radiologist", pp.35~53 (2012), Lippincott Williams & Wilkins, a Wolters Kluwer Business
- Hong EH, "Ionizing radiation induces cellular senescence of articular chondrocytes via negative regulation of SIRT1 by p38 kinase", J. Biol. Chem, 285(2), pp.1283~1295 (2010)
- Itahaha K, "Methods to detect biomarkers of cellular senescence : the senescence-associated beta-galactosidase assay", Methods Mol. Biol, 371, pp.21~31 (2007)
- Kennedy AR, "Biological Countermeasures in Space Radiation Health", Gravit. Space Biol. Bull, 16(2), pp.37~44 (2003)
- Lee EK, "Modulation of NF-κB and FOXOs by baicalein attenuates the radiation-induced inflammatory process in mouse kidney", Free Radic. Res, 45(5), pp.507~517 (2011)
- Miller J, "Proton and Heavy Ion Accelerator Facilities for Space Radiation Research", Gravit. Space Biol. Bull, 16(2), pp.19~28 (2003)
- Parsons JL, "Interplanetary crew dose rates for the August 1972 solar particle event", Radiat. Res, 153, pp.729~733 (2000)
- Todd P, "Space Radiation Health: A Brief Primer", Gravit. Space Biol. Bull, 16, pp.1~4 (2003)
- Yang NC, "A fluorimetric method using fluorescein di-b-D-galactopyranoside for quantifying the senescence-associated b-galactosidase activity in human foreskin fibroblast Hs68 cells", Anal. Biochem, 325(2), pp.337~343 (2004)
- Yang TC, "Proton radiobiology and uncertainties", Radiat. Meas, 30(3), pp.383~392 (1999)