ICR 마우스에서 감마선 조사로 유도된 조혈조직 손상 모델 확립
Establishment of γ-irradiation-induced Hematopoietic Tissue Damage Model in ICR Mice
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Ionizing radiation causes the massive generation of reactive oxygen species, resultingin cellular and tissue damage. The present study was performed to evaluate γ-irradiation inducedcellular damage in ICR mice. The mice were divided into four groups with ten mice in each group. Group 1 served as an unexposed control group. Groups 2, 3, and 4 were exposed to 3, 5, and 7Gyof γ-radiation, respectively. Five mice per group were sacrificed 1 and 7 days after γ-radiation. Exposure to γ-irradiation resulted in hematopoietic damage in a dose-dependent manner whencompared with the unexposed control group, which featured a significantly decreased spleen index. However, the exposed mice showed no significant differences in their serum AST, ALT and in thehistopathological change of their liver. These results suggest that γ-irradiation is a good tool to preparea hematopoietic damage model. This animal model can be employed to study the hematopoieticefficacy of biologically active compounds.
Abstract
Ionizing radiation causes the massive generation of reactive oxygen species, resultingin cellular and tissue damage. The present study was performed to evaluate γ-irradiation inducedcellular damage in ICR mice. The mice were divided into four groups with ten mice in each group. Group 1 served as an unexposed control group. Groups 2, 3, and 4 were exposed to 3, 5, and 7Gyof γ-radiation, respectively. Five mice per group were sacrificed 1 and 7 days after γ-radiation. Exposure to γ-irradiation resulted in hematopoietic damage in a dose-dependent manner whencompared with the unexposed control group, which featured a significantly decreased spleen index. However, the exposed mice showed no significant differences in their serum AST, ALT and in thehistopathological change of their liver. These results suggest that γ-irradiation is a good tool to preparea hematopoietic damage model. This animal model can be employed to study the hematopoieticefficacy of biologically active compounds.
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