전리 방사선의 조사가 마우스 간 세포주 BNL CL.2의 Nitric Oxide 생성에 미치는 영향

Abstract

Nitric oxide (NO) have been suggested to plays an important role in immunologic defense and influences the functioning of secretory tissues and cells. It also exhibits cytotoxicf cytostatic activity as one of major effectors of the cellular immunity system. In this study, we investigated the effects of ionized radiation on the NO production in embryonic mouse liver cell line, BNL CL.2 cells. High-dose(6 Gy) of radiation inducedproduction of NO in a dose-dependent manner from BNL CL.2 cells treated with IFN- γ. Small amounts of lipopolysaccharide (LPS) synergized with IFN- γ in the production of NO from irradiated same cells. Immunoblots showed induction of inducible nitric oxide synthase (iNOS). NO production in irradiated BNL CL.2 cells by IFN- γ or IFN- γ plus LPS was not affected by treatment with superoxide dismutase (SOD), but apparently decreased by the treatment with catalase (CAT) or SOD plus CAT. These results suggest that radiationinduced production of NO in BNL CL.2 cells might be attributed to H202 formed by ionizing radiation.