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Modulation of Hyaluronic Acid Properties by Electron Beam Irradiation
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A variety of natural polymers have been used as tissue engineering scaffolds, drug deliverysystem, and cosmetic materials due to their higher biocompatibility and water uptake. As amajor component of extracellular matrix, hyaluronic acid consisting of D-glucuronic acid and Nacetylglucosaminehas been popularly used as a hydrogel material. Even though it has good propertiesto be used in the tissue engineering and cosmetic industry, its higher viscosity has limited apotential use in a variety of applications; only low content should be applied in preparing aboveproducts. In the present study, we investigated the effect of electron beam irradiation on the propertiesof hyaluronic acid. Hyaluronic acid paste containing low contents of water changed tosolution after electron beam irradiation ranging from 1 to 10 kGy, which didn’t exhibit any alterationof surface properties and morphological change after freeze-drying. However, its viscositywas significantly decreased as absorbed dose increased, which was approximately one by hundredin comparison with the viscosity of original hyaluronic acid solution with same concentration. Inaddition, it can still interact with positive charged chitosan generating polyelectrolyte complex. Therefore, only viscosity was decreased after electron beam irradiation, whereas other propertiesof hyaluronic acid maintained. Consequently, these hyaluronic acids with lower viscosities can beused in a variety of applications in tissue engineering, drug delivery, and cosmetic industry.
Abstract
A variety of natural polymers have been used as tissue engineering scaffolds, drug deliverysystem, and cosmetic materials due to their higher biocompatibility and water uptake. As amajor component of extracellular matrix, hyaluronic acid consisting of D-glucuronic acid and Nacetylglucosaminehas been popularly used as a hydrogel material. Even though it has good propertiesto be used in the tissue engineering and cosmetic industry, its higher viscosity has limited apotential use in a variety of applications; only low content should be applied in preparing aboveproducts. In the present study, we investigated the effect of electron beam irradiation on the propertiesof hyaluronic acid. Hyaluronic acid paste containing low contents of water changed tosolution after electron beam irradiation ranging from 1 to 10 kGy, which didn’t exhibit any alterationof surface properties and morphological change after freeze-drying. However, its viscositywas significantly decreased as absorbed dose increased, which was approximately one by hundredin comparison with the viscosity of original hyaluronic acid solution with same concentration. Inaddition, it can still interact with positive charged chitosan generating polyelectrolyte complex. Therefore, only viscosity was decreased after electron beam irradiation, whereas other propertiesof hyaluronic acid maintained. Consequently, these hyaluronic acids with lower viscosities can beused in a variety of applications in tissue engineering, drug delivery, and cosmetic industry.
참고문헌 (11)
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