Effect of pH on Swelling Property of Hyaluronic Acid Hydrogels for Smart Drug Delivery Systems
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Hyaluronic acid(HA) hydrogels were synthesized by immersing HA microbeads in phosphate buffered saline solutions having different pH levels to assess the effect of pH on the swelling ratio of HA hydrogels for smart drug delivery systems. No beads were formed when the HA solution(below pH 9) was crosslinked with divinyl sulfone(DVS) because DVS is a basic solution. The variation regarding the size of the microbead was not significant, suggesting that the bead size is not a function of pH(10 ~ 14). However, the pore size of the microbeads decreased with increasing pH from 10 to 14, leading to the surface smoothness and dense network as a result of higher crosslinking. The swelling ratio of hydrogels increased when the pH rose from 2(acidic) to 6(neutral). Afterwards, it decreased with further increasing pH(basic). The lower swelling ratio may be due to the lack of ionization of the carboxyl groups. On the other hand, a higher swelling ratio is likely due to the increased electrostatic repulsions between negatively charged carboxyl groups on different chains. Experimental results suggested that pH-responsive HA hydrogels can be applicable to the controlled drug delivery systems.
Abstract
Hyaluronic acid(HA) hydrogels were synthesized by immersing HA microbeads in phosphate buffered saline solutions having different pH levels to assess the effect of pH on the swelling ratio of HA hydrogels for smart drug delivery systems. No beads were formed when the HA solution(below pH 9) was crosslinked with divinyl sulfone(DVS) because DVS is a basic solution. The variation regarding the size of the microbead was not significant, suggesting that the bead size is not a function of pH(10 ~ 14). However, the pore size of the microbeads decreased with increasing pH from 10 to 14, leading to the surface smoothness and dense network as a result of higher crosslinking. The swelling ratio of hydrogels increased when the pH rose from 2(acidic) to 6(neutral). Afterwards, it decreased with further increasing pH(basic). The lower swelling ratio may be due to the lack of ionization of the carboxyl groups. On the other hand, a higher swelling ratio is likely due to the increased electrostatic repulsions between negatively charged carboxyl groups on different chains. Experimental results suggested that pH-responsive HA hydrogels can be applicable to the controlled drug delivery systems.
참고문헌 (21)
- I. Tomatsu, "Photoresponsive hydrogels for biomedical applications", Adv. Drug Delivery Rev, 63, pp.1257~1266 (2011)
- A.K. Bajpai, "Responsive polymers in controlled drug delivery", Prog. Polymer Sci, 33, pp.1088~1118 (2008)
- H. Omidian, "Advances in superporous hydrogels", J. Control. Release, 102, pp.3~12 (2005)
- 이용일, "Preparation and characterization of nifedipine-loaded cellulose acetate butyrate based microspheres and their controlled release behavior", POLYMER BULLETIN, 65, pp.157~167, SPRINGER
- J. Kopecek, "Hydrogels as smart biomaterials", Polymer Intl, 56, pp.1078~1098 (2007)
- A.C. Jen, "Review : Hydrogels for cell immobilization", Biotech, Bioeng, 50, pp.357~364 (1996)
- W.E. Hennink, "Novel crosslinking methods to design hydrogels", Adv. Drug Deliv. Rev, 54, pp.13~36 (2002)
- A. S. Hoffman, "Hydrogels for biomedical applications", Adv. Drug Deliv. Rev, 54, pp.3~12 (2002)
- Y. Qiu, "Environment-sensitive Hydrogels for Drug Delivery", Adv. Drug Deliv. Rev, 53, pp.321~339 (2001)
- 김범상, "지능형 약물전달시스템을 위한 pH 감응형 P(MAA-co-EGMA) 수화젤 미세입자의 탑재거동", 폴리머, 32(5), pp.421~426 (2008), 한국고분자학회
- 최소영, "히알루론산의 온도감응성 그래프트 공중합체", 폴리머, 35(3), pp.223~227 (2011), 한국고분자학회
- 김진태, "연조직수복을 위한 히알루론산겔의 제조 및 평가", 생체재료학회지, 13(3), pp.105~108 (2009), 한국생체재료학회
- J. Kim, "Pyrogenicity of hyaluronic acid hydrogel cross-linked by divinyl sulfone for soft tissue augmentation", Natural Sci, 2, pp.764~768 (2010)
- G.D. Prestwitch, "Controlled chemical modification of hyaluronic acid : synthesis, applications, and biodegradation of hydrazide derivatives", J. Control. Rel, 53, pp.93~103 (1998)
- S.N. Park, "Characterization of porous collagen", hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodimide cross-linking, 2002, pp.22, 1403~1415
- 김진태, "히알루론산 마이크로비드로 형성된 하이드로겔의 이식시험", 생체재료학회지, 15(3), pp.125~128 (2011), 한국생체재료학회
- P. Gupta, "Hydrogels : from controlled release to pH-responsive drug delivery", Drug Delivery Today, 7, pp.569~579 (2002)
- K.V.R. Rao, "Swelling controlledrelease systems : recent developments and applications", Int. J. Pharm, 48, pp.1~13 (1988)
- E.J. Oh, "Control of the molecular degradation of hyaluronic acid hydrogels for tissue augmentation", J. Biomed. Mater. Res., 86, pp.685~693 (2008)
- C.E. Schante, "Chemical modifications of hyaluronic acid for the synthesis of derivatives for a broad range of biomedical applications", Carbohydrate Polymer, 85, pp.469~489 (2011)
- G.D. Prestwich, "Therapeutic applications of hyaluronic acid and hyaluronan derivatives", Pharm. Sci. Technol. Today, 1, pp.42~43 (1998)