Synthesis of Hyaluronic Acid Microsphere Crosslinked with Polyethylene Glycol Diglycidyl Ether Prepared by A Simple Fluidic Device
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Hyaluronic acid (HA) microspheres (MSs) crosslinked with polyethylene glycol diglycidyl ether (PEGDE) are prepared using a simple fluidic device (SFD) to investigate the optimized parameters. A solution mixture of PEGDE in 2-methyl-1-propanol was prepared as a continuous phase in SFD. HA solutions of 1 wt% concentration were introduced into SFD as a discontinuous phase. The HA solution prepared by stirring for more than 48 h exhib- ited spherical MSs at the needle tip inside the ring cap. As the flow rate of the continuous phase increased from 0.7 to 1.9 mL/min, the diameter of the MS decreased from 173±36 µm to 129±13 µm. Although the PEGDE concen- tration in the range of 0.2 to 1.8 vol% did not affect the diameter of the MS, the microstructure of MS, consisting of inner hollow void and wall, was changed. The inner void and wall size decreased and increased from 79.5 µm to 57.2 µm and from 10.3 µm to 21.4 µm, respectively, with increasing PEGDE concentration from 0.2 vol% to 1.8 vol%. FT-IR peaks located around 2867 cm-1 and 1088 cm-1 indicated that the HA MS prepared at different PEGDE con- centrations were chemically crosslinked. The HA MSs containing different PEGDE concentrations exhibited quan- titative cell viability of more than 98%. L-929 cells adhered well to the HA MSs and proliferated continuously with increasing culture time to 48 h regardless of PEGDE concentration, implying that the HA MSs are clinically safe and effective.
Abstract
Hyaluronic acid (HA) microspheres (MSs) crosslinked with polyethylene glycol diglycidyl ether (PEGDE) are prepared using a simple fluidic device (SFD) to investigate the optimized parameters. A solution mixture of PEGDE in 2-methyl-1-propanol was prepared as a continuous phase in SFD. HA solutions of 1 wt% concentration were introduced into SFD as a discontinuous phase. The HA solution prepared by stirring for more than 48 h exhib- ited spherical MSs at the needle tip inside the ring cap. As the flow rate of the continuous phase increased from 0.7 to 1.9 mL/min, the diameter of the MS decreased from 173±36 µm to 129±13 µm. Although the PEGDE concen- tration in the range of 0.2 to 1.8 vol% did not affect the diameter of the MS, the microstructure of MS, consisting of inner hollow void and wall, was changed. The inner void and wall size decreased and increased from 79.5 µm to 57.2 µm and from 10.3 µm to 21.4 µm, respectively, with increasing PEGDE concentration from 0.2 vol% to 1.8 vol%. FT-IR peaks located around 2867 cm-1 and 1088 cm-1 indicated that the HA MS prepared at different PEGDE con- centrations were chemically crosslinked. The HA MSs containing different PEGDE concentrations exhibited quan- titative cell viability of more than 98%. L-929 cells adhered well to the HA MSs and proliferated continuously with increasing culture time to 48 h regardless of PEGDE concentration, implying that the HA MSs are clinically safe and effective.
참고문헌 (28)
- 전철병, "Viscoelasticity of Hyaluronic Acid Dermal Fillers Prepared by Crosslinked HA Microspheres", 폴리머, 40(4), pp.600~606 (2016), 한국고분자학회[DOI]
- Schante CE, "Chemical modifications of hyaluronic acid for the synthesis of derivatives for a broad range of biomedical applications", Carbohydr. Polym, 85, pp.469~489 (2011)
- 김진태, "Tissue Response to Implants of Hyaluronic Acid Hydrogel Prepared by Microbeads", 조직공학과 재생의학, 11(1), pp.32~38 (2014), 한국조직공학과 재생의학회[DOI]
- Robert MJ, "Chemistry for peptide and protein PEGylation", Adv. Drug Deliv. Rev, 54(4), pp.459~476 (2002)
- 이득용, "Influence of Molecular Weight on Swelling and Elastic Modulus of Hyaluronic Acid Dermal Fillers", 폴리머, 39(6), pp.976~980 (2015), 한국고분자학회[DOI]
- Flynn TC, "Comparative histology of intradermal implantation of mono and biphasic hyaluronic acid fillers", Dermatol. Surg, 37, pp.637~ (2011)
- Obata H, "Synthesis and evaluation of hyaluronic acid hydrogels modified with various crosslinkers as biodegradable polymers", J. Appl. Polym. Sci, 134, pp.454453~ (2017)
- Zerbinati N, "Toward physicochemical and rheological characterization of different injectable hyaluronic acid dermal fillers cross-linked with polyethylene glycol diglycidyl ether", Polym, 13, pp.948~ (2021)
- Jeong CH, "In vitro toxicity assessment of crosslinking agents used in hyaluronic acid dermal filler", Toxicol. in Vitro, 70, pp.105034~ (2021)
- Monticelli D, "Chemical characterization of hydrogels crosslinked with polyethylene glycol for soft tissue augmentation", Open Access Maced J. Med. Sci, 7, pp.1077~1081 (2019)
- 김진태, "Biocompatibility of Hyaluronic Acid Hydrogels Prepared by Porous Hyaluronic Acid Microbeads", Metals and Materials International, 20(3), pp.555~563 (2014), 대한금속·재료학회[DOI]
- Robert MJ, "Chemistry for peptide and protein PEGylation", Adv. Drug. Deliv. Rev, 54(4), pp.459~476 (2002)
- 이혜란, "Poly(ε-caprolactone) Microcapsule with Encapsulated Nifedipine Prepared by Magnetic Stirrer", 의공학회지, 40(1), pp.7~14 (2019), 대한의용생체공학회
- 임현주, "Drug Delivery Behavior of PCL and PCL", PEG Microcapsules Prepared by High-speed Agitator and Syringe Pump, 2020, pp.44(4), 487~494[DOI]
- 정다연, "Hollow Poly(lactic acid) and Poly(lactic acid)", polyethylene glycol Microspheres Prepared by A Simple Fluidic Device, 2021, pp.42(2), 62~70[DOI]
- Choi S, "Preparation of uniform microspheres using a simple fluidic device and their crystallization into close-packed lattices", Small, 5, pp.454~459 (2009)
- Baek SW, "Onestep fabrication of uniform biodegradable microbeads with unimodal and bimodal porous structures using spontaneous microphase separation", Macromol. Mater. Eng, pp.1800139~ (2018)
- 김주환, "Fabrication of Poly(L-lactide) Porous Beads Coated with Hydroxyapatite Using a Simple Fluidic Device for Tissue Engineering", Macromolecular Research, 23(6), pp.501~504 (2015), 한국고분자학회[DOI]
- 전철병, "Effect of molecular weight of hyaluronic acid (HA) on viscoelasticity and particle texturing feel of HA dermal biphasic fillers", 생체재료학회지, 20(4), pp.275~281 (2016), 한국생체재료학회[DOI]
- Yo-Seung Song, "Effect of calcination temperature on photocatalytic activities of Er-TiO2 nanotubes", Journal of Ceramic Processing Research, 20(2), pp.182~186 (2019), 청정에너지연구소[DOI]
- 신지연, "Effect of CMC Concentration on Cell Growth Behavior of PVA", CMC Hydrogel, 2020, pp.28(9), 813~819[DOI]
- Shin J, "Effect of polyethylene glycol molecular weight on cell growth behavior of polyvinyl alcohol", carboxymethyl cellulose, J. Appl. Polym. Sci, pp.2020, 137(48)[DOI]
- Kim S, "Characteristics and cell growth behavior of gelatin", PVA porous scaffolds, 2021, pp.13(5), 883~888
- Longhao J, "Visible light-cured antibacterial collagen hydrogel containing water-solubilized triclosan for improved wound healing", Mater, 14(9), pp.2270~ (2021)
- Kim D, "Mechanical properties, phase stability, and biocompatibility of (Y,Nb)-TZP", Al2O3 composite abutments for dental implant, 2000, pp.53(4), 438~443
- 정희석, "Mechanical Properties and Cytotoxicity of PLA", PCL films, 2018, pp.8(3), 267~272[DOI]
- Vasi A, "Chemical functionalization of hyaluronic acid for drug delivery applications", Mater. Sci. Eng. C, 38, pp.177~185 (2014)
- Song, R, "The Structure and Properties of Chitosan", Polyethylene, 2008, pp.26, 621~630