마우스에서 하수오 열수추출물이 발모관련 효소활성과 유전자 발현에 미치는 영향
The Effect of Polygoni mulitiflori Radix Water Extracts on Enzyme Activities and Gene Expression Relevant to Hair Growth in C57BL/6 Mice
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
This study was carried out to investigate the effect of Polygoni mulitiflori Radix water extracts (PMWE) on enzyme activities and gene expression relevant to hair growth in C57BL/6 mice. Six weeks old female mice were divided into 4 groups including normal group (saline, N), vehicle control group (jojoba oil+100% ethanol+saline, VC), positive control group (3% minoxidil, PC), and experimental group (PMWE, E), and they were topically applied with an amount of 0.2 mL experimental compounds once a day, 6 days a week for 4 weeks. Hair regrowth was evaluated by the gross examination. Also the activities of enzymes including ALP and γ-GT and the mRNA expression quantity of IGF-1 in the skin of mice were evaluated. Topical treatment of PMWE and 3% minoxidil to dorsal skin accelerated hair regrowth faster than N and VC groups. Skin ALP and γ-GT activities were significantly higher in PC and E groups compared with N and VC groups in week 4 (p<0.05). IGF-1 mRNA expression in the skin was significantly higher in E group compared with N, VC and PC groups in week 4 (p< 0.05). These results indicate that PMWE effectively promoted hair growth in an animal model via increase in enzyme activities and gene expression relevant to hair growth.
Abstract
This study was carried out to investigate the effect of Polygoni mulitiflori Radix water extracts (PMWE) on enzyme activities and gene expression relevant to hair growth in C57BL/6 mice. Six weeks old female mice were divided into 4 groups including normal group (saline, N), vehicle control group (jojoba oil+100% ethanol+saline, VC), positive control group (3% minoxidil, PC), and experimental group (PMWE, E), and they were topically applied with an amount of 0.2 mL experimental compounds once a day, 6 days a week for 4 weeks. Hair regrowth was evaluated by the gross examination. Also the activities of enzymes including ALP and γ-GT and the mRNA expression quantity of IGF-1 in the skin of mice were evaluated. Topical treatment of PMWE and 3% minoxidil to dorsal skin accelerated hair regrowth faster than N and VC groups. Skin ALP and γ-GT activities were significantly higher in PC and E groups compared with N and VC groups in week 4 (p<0.05). IGF-1 mRNA expression in the skin was significantly higher in E group compared with N, VC and PC groups in week 4 (p< 0.05). These results indicate that PMWE effectively promoted hair growth in an animal model via increase in enzyme activities and gene expression relevant to hair growth.
참고문헌 (39)
- 김휘준, "남성형 탈모증 환자의 질병 행동 특성과 삶의 질에 관한 연구", 대한피부과학회지, 39(10), pp.1094~1099 (2001)
- 도윤정, "골다공증에 대한 하수오 물 추출물의 효력 평가", 박사, pp.2007
- 오수진, "모근세포의 분리배양과 발모촉진 활성에 대한 연구", 석사, pp.2008
- 이계호, "모발생장기 유도 C3H생쥐에 있어서 미녹시딜과 생약추출 혼합 조성물의 모발 재성장 유도 효능", 약 학 회 지, 47(1), pp.14~19 (2003), 대한약학회
- 이영중, "하수오가 고지혈증 흰쥐의 혈중지질 및 효소활성에 미치는 영향", 한국한약학회지, 14(1), pp.69~77 (1999)
- 이윤경, "韓藥抽出物의 發毛效果에 關한 硏究 : 何首烏, 當歸 및 枸杞子 混合物의 發毛效果", 대한본초학회지, 19(2), pp.83~90 (2004), 대한본초학회
- 정춘근, "연년익수고본단의 육모 효능에 대한 면역조직화학적 연구", 박사, pp.2008
- 허진주, "C57BL6 마우스에서 복합생약제인 황금궁의 육모 효과", 약 학 회 지, 49(6), pp.518~526 (2005), 대한약학회
- 홍정애, "익기보혈탕이 C57BL", 6 마우스의 육모촉진 및 Cytokine 변화에 미치는 영향, 경희대학교 대학원, 2008
- Bol DK, "Overexpression of insulin-like growth factor-1 induce hyperplasia dermal abnormalities and spontaneous tumor formation in transgenic mice", Oncogene, 14(14), pp.1725~1734 (1997)
- Buhl AE, "Potassium channel conductance as a control mechanism in hair follicles", Journal of Investigative Dermatology, 101(1), pp.148~152 (1993)
- Buxman MM, "Differentiation markers in fetal epidermis: Transglutaminase and transpeptidase", Journal of Investigative Dermatology, 72(4), pp.171~177 (1979)
- Cash TF, "Psychological effects of androgenetic alopecia on women: comparisons with balding men and with female controls", Journal of American Academy Dematology, 29(4), pp.568~575 (1993)
- Clark RL, "External genitalia abnormalities in male rats exposed in utero to finasteride, a 5α-reductase inhibitor", Teratology, 42(5), pp.483~496 (1990)
- Hagemann T, "Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution", Contact Dermatitis, 53(1), pp.85~97 (2005)
- Hamada K, "Pro- inflammatory cytokine interleukin-1α is downregulated during anagen phase of hair cycle in vivo", Journal of Dermatological Science, 33(3), pp.195~198 (2003)
- Harada N, "Effect of topicalapplication of raspberry ketone on dermal production of insulin-like growth factor-1 in mice and on hair growth and skin elasticity in humans", Growth Hormone & IGF Research, 18(4), pp.335~344 (2008)
- Hatano T, "Phenolic constituents of cassia seeds and anthrsquinones on methicillin-resistant staphylococcus aureus", Chemical and Pharmaceutical Bulletin, 47(8), pp.1121~1127 (1999)
- Hattori M, "Biochemical analysis of hair growth from the aspects of aging and enzyme activities", Journal of Dermatology, 10(1), pp.45~54 (1983)
- Hibino T, "Role of TGF-β2 in the human hair cycle", Journal of Dermatological Science, 35(1), pp.9~18 (2004)
- Huang HC, "Vasorelaxants from chinese herhs,emodin and scoparone, possess immunosuppressive properties", European Journal of Dermatology, 198(2), pp.211~213 (1991)
- Itami S, "Androgen induction of follicular epithelial cell growth is mediated via insulin-like growth factor-1 from dermal papilla cells", Biochemical and Biophysical Research Communications, 212(3), pp.988~994 (1995)
- Kawabe TT, "Use of γ-glutamy1 transpeptidase activity as a marker of hair cycle and anagen induction in mouse hair follicles", Journal of Investigative Dermatology, 103(1), pp.122~126 (1994)
- Kim CD, "Interon β secreted from human hair dermal papilla cells inhibits the growth of outer roots heath cells cultured in vitro", Journal of Biochemical and Biophysical Research Communications, 290, pp.1133~1138 (2002)
- Lishung LV, "Antioxidnt activity of stilbene glocoside from Polygonum multiflorum Thunb in vivo", Journal of Food Chemistry, 104(4), pp.1678~1681 (2007)
- Lurie R, "Laron syndrome (primary growth hormone insensitivity): a unique model to explore the effect of insulin-like growth factor-1 deficiency on human hair", Dermatology, 208(4), pp.314~318 (2004)
- Machiko L, "Hair cycle dependent changes of alkaline phosphatase activity in the mesenchyme and epithelium in mouse vibrissal follicles", Development & Growth Differentiation, 49(3), pp.185~195 (2007)
- Mackay A, "Minoxidil in the management of intractable hypertension", Quarterly Journal of Medicine, 50(198), pp.175~190 (1981)
- McClellan KJ, "Finasteride: a review of its use in male pattern hair loss", Drugs, 57(1), pp.111~126 (1999)
- Meister A, "Glutathione", Annual Review of Biochemistry, 52, pp.711~715 (1983)
- Nguyen KH, "Pseudoacromegaly induced by the long-term use of minoxidil", American Academy of Dermatology, 48(6), pp.962~965 (2003)
- Randall VA, "Do androgens influence hair growth by altering the paracrine factors secreted by dermal papilla cells?", European Journal of Dermatology, 11(4), pp.315~320 (2001)
- Reynolds AJ, "Dermal cell populations show variable competence in epidermal cell support: stimulatory effects of hair papilla cells", Journal of Cell Science, 98(1), pp.75~83 (1991)
- Stenn KS, "Controls of hair follicle cycling", Physiological Reviews American Physiological Society, 81(1), pp.449~494 (2001)
- Stough D, "Psychological effect, pathophysiology and management of androgenetic alopecia in men", Mayo Clinic Proceedings, 80(10), pp.1316~1322 (2005)
- Trüeb RM, "Molecular mechanism of androgenetic alopecia", Experimental Gerontology, 37(8), pp.981~990 (2002)
- Wang W, "Effect of polygonum multiflorum on the life-span and lipid metabolism in senile Japanese quails", China Journal of Modern Developments Traditional Medicine, 8(4), pp.223~224 (1988)
- Weger N, "IGF-1 signalling controls the hair growt cycle and the differentiation of hair shafts", Journal of Investigative Dermatology, 126(5), pp.873~882 (2006)
- Yin JH, "Pharmacological and clinical studies on the processed products of radix a Polygoni multiflori", China Journal of Chinese Materia Medica, 17(12), pp.722~724 (1992)