어성초를 포함한 천연복합추출물의 C57BL/6 마우스에 대한 발모효과
Effect of Herbal Complex Extract Including Houttuynia cordata Thunb on Hair Growth Promotion in C57B/6 Mice
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초록
Present study was carried out to investigate the effect of herbal complex extract including Houttuynia cordata Thunb on hair growth and gene expression in C57BL/6 mice. Twenty mice were divided into 4 experimental groups including normal (control), 50% herbal complex extract (S-1), 100% herbal complex extract (S-2) and 5% minoxidil-treated group. The test materials were daily applied with 200 μl per mouse on shaved dorsal skin for 3 weeks. The hair growth was monitored by photograph at 7, 14 and 21 days after topical application. Then the changes of hair density and hair thickness in the hair-removed area were evaluated by phototrichogram using folliscope. Also the expression level of growth factors related to hair growth was measured by real time polymerase chain reaction. Application of S-2 or minoxidil-treated group stimulated the hair growth compared to vehicle treatment. Therefore hair density of S-2 or minoxidiltreated group application was increased about 2.5 and 3.1 fold more than in vehicle application on 21 day, respectively. And hair weight of both S-2 and minoxidil-treated group were increased about 8.3 and 10.0 fold more than in vehicle spreading on 21 day, respectively. Futhermore the gene expression of insulinlike growth factor (IGF)-1 and vascular endothelial growth factor (VEGF) were significantly up-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. However the gene expression of transforming growth factor (TGF)-β1 was not significantly down-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. These data suggest that S-2 has potent stimulating activity on hair growth in C57BL/6 mice and potential usefulness as ingredients of hair tonic and hair restore.
Abstract
Present study was carried out to investigate the effect of herbal complex extract including Houttuynia cordata Thunb on hair growth and gene expression in C57BL/6 mice. Twenty mice were divided into 4 experimental groups including normal (control), 50% herbal complex extract (S-1), 100% herbal complex extract (S-2) and 5% minoxidil-treated group. The test materials were daily applied with 200 μl per mouse on shaved dorsal skin for 3 weeks. The hair growth was monitored by photograph at 7, 14 and 21 days after topical application. Then the changes of hair density and hair thickness in the hair-removed area were evaluated by phototrichogram using folliscope. Also the expression level of growth factors related to hair growth was measured by real time polymerase chain reaction. Application of S-2 or minoxidil-treated group stimulated the hair growth compared to vehicle treatment. Therefore hair density of S-2 or minoxidiltreated group application was increased about 2.5 and 3.1 fold more than in vehicle application on 21 day, respectively. And hair weight of both S-2 and minoxidil-treated group were increased about 8.3 and 10.0 fold more than in vehicle spreading on 21 day, respectively. Futhermore the gene expression of insulinlike growth factor (IGF)-1 and vascular endothelial growth factor (VEGF) were significantly up-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. However the gene expression of transforming growth factor (TGF)-β1 was not significantly down-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. These data suggest that S-2 has potent stimulating activity on hair growth in C57BL/6 mice and potential usefulness as ingredients of hair tonic and hair restore.
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